Staph aureus

Strept pyogenes

Strept viridans

Strept pneumonia e

Strept faecalis (enterococci)

Neisseria gonorrhea

Neisseria meningitidis

Morphol ogy capsulated In clusters Culture charact er FA & 37 C CO2: 0.03% Nutrient agar golden yellow endopigment Blood agar hemolysis Mannitol salt agar (selective media)

G+ve cooci Non motile & non spore forming Non capsulated Non capsulated
capsulated

G-ve cocci intracellular

capsulated In pairs

In long chain

In pairs

In short chain 0.03% Grow on nutrient agar Blood agar hemolysis

5-10% Fastidious and not grow on nutrient agar

5-10% Fastidious and not grow on nutrient agar nor blood agar Grow on chocolate agar Grow on Thayer martin media (selective media)

Blood agar hemolysis

Blood agar hemolysi s

Biochem ical Reactio n

Catalase +ve Coagulase +ve Mannitol fermentation

Sensitive to bacitracin 0.04

Blood agar hemolysis & Mucoid colonies with central depression Catalase ve Ferment Ferment rafinose inuline Bile soluble

grow in bile Grow in 6.5% NaCl broth

Glucose fermentation acid Oxidase +ve Not ferment maltose Maltose fermentation

acid DNase +ve

acid Sensitive to optochain pneumonia - otitis media meningitis Esculine hydrolysis Flora of GIT UTI - Infective endocarditi s - Nosocomi al infection Gonorrhea (STD ) -urethritis - cervicitis -Ophthalamia neonatorum Cerebrospinal meningitis

Disease s

Suppurative: as carbuncle & fruncle & bacteremia & nosocomial infection Toxogenic as food poisoning & scalded skin disease & toxic shock syndrome

Sample

Pus & food remnant & stool & vomitus

Suppurative: tonsillitis & otitis media & pneumonia & bacteremia & meningitis & impetigo Toxogenic : scarlet fever & toxic shock syndrome Sequalae: Rheumatic fever & glomerulo-nephritis & poly arthritis Pharyngeal swab

-Flora of mouth dental caries - on tooth extracti on in diseased heart infective endocard itis Blood Sputum & blood blood culture blood culture & CSF

Specific

In food poisoning : Bacteriophage typing to trace source of

In scarlet test: 1. Schultz Charlton test rapid diagnosis (toxin antitoxin

intraperito neal injection into mice 2

Urine & blood Urethral blood discharge & culture cervical swab & eye swab & in chronic cases morning drop In chronic cases detect specific antibodies in

case: CSF through lumbar puncture - carrier nasopharyng eal swab Rapid diagnosis by direct agglutination test: react CSF with monoclonal -

infection Antimicrobial susceptibility: To select the efficient antibiotiv

neutralization test) inject antitoxin neutralize erythrogenic toxin rash disappear within 12 hours 2. dick test: diagnose susceptibility(to xin antitoxin neutralization test) in sequalae ASO & C reactive protein & ESR

pneumonia death quelling reaction: specific antibodies bind with capsule capsular swelling

pts serum by ELISA Not pathogenic for laboratory animal

Ab against meningiococci