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Three Distinct New Viruses for the Disease and Death of Seals and Dolphins Morbilliviruses
In Family
Paramyxoviridae Serologically related, single-stranded RNA viruses
Until 1988
Canine distemper virus (CDV) Measles virus (MV) Rinderpest virus (RPV) Peste des petites ruminants virus (PPRV)
DMV and PMV most closely related to RPV and PPRV DMV and PMV are strains of same viral species PDV and Baikal seal morbillivirus closely related to CDV
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1988, U.S.A. East Coast Hundreds of bottlenose dolphin 1988, NW Europe 20,000 harbor seals Several hundred gray seals 1987-8 Lake Baikal, Thousands of Baikal seals Siberia 1987-8 Mediterranean sea Thousands of striped dolphins 1993-4, Gulf of Mexico Scores of bottlenose dolphins
Atlantic coast bottlenosed dolphin epizootic Northwestern Europe: harbor seals, harbor porpoises Western Mediterranean: striped dolphins Gulf of Mexico; Bottlenose dolphins Eastern pacific common dolphins
1998 1993-1994
Contact with arctic seal species most likely source (harp seals migrating south)(PDV-1) Lake Baikal Siberian seals acquired canine distemper virus (PDV-2) from land animals
http://courses.iddl.vt.edu/AEID_I/pdf/web/12Pfeiffer_NandQ.htm
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Long island harbour seals NW Europe harbour porpoises Western Atlantic harp seal and pilot whales
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Carcass Examination
Microscopic evidence of nuclear and cytoplasmic acidophilic inclusion bodies in diverse cells (e.g., CNS, lymphoid, epithelial)
Development of Monoclonal Antibodies for Differential Diagnosis of Morbilliviruses in Marine Mammals Diagnosis in Live Animals
Cytologic and immunofluorescence examination of conjunctival or nasal smears for inclusion bodies or morbillivirus antigen Stage of infection, titer of antibody Distribution and frequency of DMV-antigen in organs of 57 Striped dolphins (Stenella coeruleoalba) *Positive cases/total number of dolphins investigated for this tissue or organ
Sample Questions: 1). The dolphin morbillivirus has been shown to possess structural similarity to all but which one of the following? a. Measles virus b. Feline leukemia virus c. Phocine morbillivirus 2). Observations of afflicted animals in the Mediterranean striped dolphin and/or Atlantic bottlenose dolphin epizootics have included all but which of the following?
http://courses.iddl.vt.edu/AEID_I/pdf/web/12Pfeiffer_NandQ.htm Page 4 of 5
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a. Exudative bronchial pneumonia b. Septicemia c. Ocular opacity 3). Morbillivirus - positive (DMV or PMV) serology has been observed in all but which of the following species? a. Sea otters b. Atlantic bottlenose dolphins c. Pilot whales and manatees 4) Please indicate which of the following comments is not correct. a. PMV is known to infect dolphins b. The lungs and bronchial system are primary targets of MV in marine mammals c. MV frequently attacks the brain of marine mammals
http://courses.iddl.vt.edu/AEID_I/pdf/web/12Pfeiffer_NandQ.htm
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