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Serine Proteases

The Serine Proteases

Chymotrypsin, trypsin, and elastase are three enzymes that cleave protein chains. Each has its own selectivity: that is, they cleave proteins at different structure points They have very strong structural similarity They use the same mechanism for the cleavage, a mechanism that very much resembles the mechanism we would write for the acid-catalyzed hydrolysis of an amide First, the structural similarity: Superposition of Trypsin (yellow), Elastase (green), and Chymotrypsin (blue) Backbones

The three amino acids picked out in red are the three that actually do the catalysis. Here are sequence alignments for chymotrypsin (5cha) and trysin (5ptp), and elastase (1est) and trypsin (1tld) produced by the Swiss PDB Viewer: Chymotrypsin vs Trypsin Elastase vs Trypsin

The asterisks mark identical residues, and the dots mark very similar side chains, such as Leu and Ile, or Phe and Tyr

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Serine Proteases

Deep View calculates an RMS difference between chymotrypsin and trypsin of only 0.94 A, and 1.14 A for the elastase-trypsin pair, reflecting the obvious matches in the graphics above What we see here is a clear example of divergent evolution. All are derived from a common ancestral serine protease, and are described as homologous. Taking a step further back, one finds that some non-mammalian serine proteases have 20-50% sequence identity with mammalian ones, suggesting a common ancestral protease an evolutionary step further back. Evolution can converge on functionality also. The first crystal structure of a bacterial serine protease, subtilisin, from B. amyloliquefaciens, shows a thoroughly different construction from the mammalian ones, and essentially no sequence homology: Subtilisin (B. amyloliquefaciens)

The orange balls are Ca++ , providing thermal stability But the enzymes are functionally identical; subtilisin uses the same three catalytic residues, shown in red: Asp32, His64, and Ser221. The mechanism of catalysis is the same, including the positioning of substrate by hydrogen bonding. This appears to be a case of convergent evolution: Mother Nature found a good idea a second time. As we have seen in several pictures now, the catalytic work of the proteases is done by the so-called catalytic triad, Asp102, His57, and Ser195:

Here is the triad picked out from the crystal structure of chymotrypsin (the extra fine black lines are artifacts of my effort to hide the rest of the enzyme). Remember that X-rays can't see hydrogen atoms; we have to infer their positions.

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Serine Proteases

The mechanism outlined below applies to all of the serine proteases, with small variations. We start with the binding, in cartoon form.

The next step appears to be His57 removing a proton from the the Ser105 OH, while the O does a nucleophilic attack on the peptide carbonyl:

The shift of negative charge onto the carbonyl oxygen of the peptide is facilitated by hydrogen bonding of the oxygen to the backbone NH groups of Ser195 and Gly193 These form what is called "the oxyanion hole" We now have a structure equivalent to the tetrahedral intermediate in the non-enzymatic mechanism The biggest difference is that the two protons that are part of the OH groups are merely hydrogen-bonded in the enzyme Whether a tetrahedral intermediate is formed was a point of considerable contention in the early investigations of protease mechanisms; biochemists tended to draw nucleophilic acyl substitutions as if they were SN2 reactions - all one step. This ignored a fundamental difference between the two kinds of reaction. Nucleophilic substitution, in frontier orbital terms, involves an interaction between the HOMO of the nucleophile and the LUMO of the

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electrophile (substrate)

Serine Proteases

Alkyl substrates, which undergo SN2 reactions have much different LUMOs than acyl substrates, which form tetrahedral intermediates. To illustrate, here's the LUMO of ethyl chloride, CH3CH2Cl: LUMO of Ethyl Chloride

You can see that it involves chiefly the C-Cl bond, and has a considerable back lobe where the nucleophile interacts. Now here is the LUMO of acetyl chloride, CH3(C=O)Cl: LUMO of Acetyl Chloride

This LUMO is almost exclusively on the C=O, and hence there is no way an attacking nucleophile can break the C-Cl bond directly. In short, a tetrahedral intermediate MUST form because the orbital construction of the substrate won't permit any other pathway. End of story. OK, so we've got the tetrahedral intermediate. Where next? Here's the tetrahedral intermediate, bound to Ser195 with the former carbonyl oxygen in the "oxyanion hole".

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The next step is the reconstruction of the carbonyl double bond, with expulsion of the leaving group - in this case, the rest of the protein. This is the stage at which the protein chain actually is cleaved, and it produces an "acyl enzyme": the acyl part of the peptide that was cleaved, bound as an ester to Ser195.

OK, now we've got to cleave the acyl enzyme; enzyme are catalysts, and are not permanently altered in the reaction. To do this, we need a molecule of water. From here on out, we're writing the mechanism for hydrolysis of an ester:

Restore the carbonyl double bond:

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Serine Proteases

This releases the other end of the original protein, and restores the catalytic triad to its beginning state:

Dissociation of the second protein fragment leaves the enzyme ready to go again.

This page last modified 10:31 AM on Sunday April 27th, 2003. Webmaster, Department of Chemistry, University of Maine, Orono, ME 04469

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