Contents

Section 1: Installing the Software1 Section 2: Installing the Driver4 Section 3: Precise Calibration 11 Section 4: Capturing images with the Capture Window 16 Section 5: Capturing Video with the Capture Window 18 Section 6: Taking Measurements19 Section 7: Customizing Your Settings 20 Section 8: Adding Sound to Your Files 21 Section 9: How to Play Sound21 Section 10: How to Combine Images22 Section 11: Introduction to MCCamera23 Section 12: Frequently Asked Questions28

Note: The intention of this guide is to provide quick instructions to key tasks of the Motic Images Plus software. If more detail is needed or what you are looking for is not contained in this guide, please consult the documentation that comes with your microscope or visit our website http://www.motic.com.

Installing the Software

Before installing Motic Images Plus 2.0 ML, please read this chapter carefully, and perform setup by following the step-by-step instructions.

Preparation for software installation

1. 2. Get your Motic microscope ready, and check that the USB cable of microscope is not connected to the USB port of the computer. Microscope is powered up.

Installing the software

Follow the instructions outlined below to install Motic Images Plus 2.0 ML (images from Windows 98SE, similar processes for Windows XP, ME and 2000).

Insert the CD-ROM into your CD-ROM drive and the setup wizard appears automatically.

The Motic Images Plus 2.0 ML Installer prompts Setup being processed.

Get ready for installation as the installer informs and click Next.

To install Motic Images Plus 2.0 ML, you must accept all the terms of the Software License Agreement. If you agree, click Yes to continue. If you do not, click No and the installation will be cancelled.

Please fill in the blanks with your name and company name before continuing with the installation process. Click Next.

The Choose Destination Location dialog box will appear. Accept the default destination path C:\ Program Files\ Motic\ Motic Images Plus 2.0 or click Browse to select your own path. Then click Next.

Select one of the 3 Setup types to install; before file copying starts, you may review or change the settings by clicking Back. Begin program installation by clicking Next.

Accept the default program folder Motic, or specify a different folder by entering your own folder name or selecting from an existing folder and click Next.

Copying of program files begins. (If you wish, you can choose to install the PDF reader before the copying of the program files starts. If you already have the PDF reader installed, you can skip this part.

When all the program files have been copied to your computer, click Finish .It is highly recommended that you restart your computer.

Installing the Driver

Installing the driver in Windows 2000:
Connect the USB cable to the computer USB port and the following dialog box appears.

Insert the Motic Images Plus 2.0 ML disk into the CD-ROM and click OK.

Click Next.

Choose Search for the and click Next.

Choose Specify a Location, click Next and then click Browse.

Choose 2e under Driver in the CD-ROM if your microscope system is Camera 2 (or select the corresponding driver according to the instructions on the CD case, or see the chart below) and click OK.
Camera 2 Camera 3 PAL USB1 only USB1, RCA Camera 3 NTSC USB1, RCA MC1000/MC2000 USB2.0 only, MC1000/MC2000 S-Video, AD640 S-Video, 2e M210

1.3/2.0 Megapixels MC1001/MC2001 USB2.0 only, Motic MC Camera V3 Motic MC Camera V3 MC351/MC352

1.3/2.0 Megapixels MC3001 USB2.0 only, 3.0 Megapixels MC351/MC352 USB1 only

Click Next.

Click Yes.

Files are being copied to your computer.

Click Finish.

Installing the Motic CapL3 Driver

(If you have not yet purchased a capture card from us, such as L311, L312, L320 etc, please skip the following text.)

Left is the installation location of the MoticCap L3xx, the PC1 slot, to which the contact footing of the said card is to be inserted.

Press the card down with the input sockets plate attached to the sockets panel (usually at the back) of your computer.

Click Next.

Choose Search for a and click Next.

Choose CD-ROM drives and click Next.

Click Next.

Click Yes.

Click Finish.

Click Yes.

Click Next.

Choose Search for a and click Next.

Choose CD-ROM drives and click Next.

Click Next.

Click Yes.

Click Finish.

Restart your computer by clicking Yes.

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Precise Calibration
Calibration is a very important step to be taken before using the Motic Images Plus 2.0 ML measuring system as it will ensure the accuracy of the systems measurements. Click on the drop-down arrow at the right of the Calibration button on the Measure tool bar to display the following drop-down menus.

Select the Calibration Wizard command to display the Calibration Wizard window. To select a calibration method, click on the corresponding tab.

Motic Images Plus 2.0 ML provides three calibration methods, Calibration with Circle, Calibrate with Scale Cross and Calibrate with Scale Line. 1. Calibrate with Calibration Circle ( i ) To calibrate with a calibration circle, select the Calibration Wizard command to display the Calibration Wizard window. Click on the Calibrate with Calibration Circle tab to display the corresponding panel. Click Load Image to display the Open Image files dialog box from which calibration circles may be selected for calibration. Click Open to load the selected image.

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( ii )

Confirm the objective lens used to capture

the selected image, then input the diameter of the calibration circle. Click Calibration to complete the calibration process.

Note: Use the appropriate calibration circle slide and magnification suited to your microscope.

( iii )

The Save Sign dialog box will display

after clicking "Calibration". Click "Save" in the dialog box to save the calibration results and use them for measuring.

( iv ) When calibration is complete, click Close to close the Calibration Wizard window. 2. Calibrate with Scale Cross ( i ) To calibrate with a scale cross, select the Calibration Wizard command to display the Calibration Wizard window. Select the Calibrate with Scale Cross tab to display the corresponding panel. Click on Load Image to display the Open Image files dialog box from which scale crosses may be selected for calibration. Click Open to load the selected images.

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( ii )

When an image is loaded, a circle will to display the

appear on it. Modify its color by clicking the Circle Color button selected. First, place the center of the circle (set as "O") in the middle of the scale cross. Next, place a point (set as "A") that is horizontal with the center of the circle on the horizontal line and place a point (set as "B") that is vertical with the center of the circle on the vertical line. The magnifier window under the image window will help users accurately place the points. Confirm the objective lens used to capture the selected image and then input the actual length of "OA" in the "Width" bar and the actual length of "OB" in the "Height" bar. Click Calibration to complete the calibration process. Color Palette where a color for the circle may be

( iii ) The Save Sign dialog box will display after clicking "Calibration". them for measuring. Click "Save" in the dialog box to save the calibration results and use

( iv ) When calibration is complete, click Close to close the Calibration Wizard window.

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3.

Calibrate with Scale Line ( i ) To calibrate with a scale line, select the Calibration Wizard command to display the Calibration Wizard window. Select the Calibrate with Scale Line tab to display the corresponding panel. First, select the Horizontal tab to load horizontal scale lines. Click Load Image to display the Open Image files dialog box from which horizontal lines may be selected for calibration. Click Open to load the selected lines.

( ii )

Next, select the Vertical tab to load vertical

scale lines. Click Load Image to display the Open Image files dialog box from which vertical lines may be selected for calibration. Click Open to load the selected lines.

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( iii ) When the image is loaded, a line will appear on it. Modify its color by clicking the Line Color to display the Color Palette button where a color for the line may be chosen. Drag the two ends of the line (set as "A" on the horizontal line and as "B" on the vertical line) to two different scales. The magnifier window under the image window will help users accurately place the points. Confirm the objective lens used to capture the selected images and then input the actual length of "A" in the "Width" bar and the actual length of "B" in the Height bar. Click Calibration to complete the calibration process.

( iv ) The Save Sign dialog box will display after clicking "Calibration". Click "Save" in the dialog box to save the calibration results and use them for measuring.

( vi ) When calibration is complete, click Close to close the Calibration Wizard window.

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Capturing Images with the Capture Window

1. When Motic Images Plus 2.0 ML is run, the following workspace will display:

Plus Style 2.

Standard Style

Click the Capture Window button (pointed out by the red arrow) on the toolbar.

3.

The

Capture

Window

will

then

display.

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4.

Click the Capture Current Image button located on the window toolbar to capture the current microscope image.

5.

After capturing an image, the workspace will display as follows:

Plus Style 6.

Standard Style

Next, select the Save As command from the File menu to display the Save as dialogue box. Save the image under a user defined filename and select a file format from the drop down list provided.

Plus Style Note:

Standard Style

If you experience any problems obtaining a live image, consult Frequently Asked Questions for troubleshooting tips.

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Capturing Video with the Capture Window

1.

Open the Capture Window and select Set Time Limit from the Capture menu.

2.

In the Time Limit dialogue box, define a time limit for recording and click OK.

3.

Click the Start Capture Video button from the toolbar located on the left of the image window.

4.

Next, enter a name for your video and click Save. Once you click Save, a dialog box will appear, click OK to return to the capture window where recording will begin.

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Taking Measurements
Note:
First, make sure that your system has been properly calibrated prior to taking any measurements.

Measuring
1. Select any of the Measurement tools by clicking on the corresponding button on the Measure tool bar.

Plus Style 2.

Standard Style

From the Measure Control Panel, select the magnification of the objective lens used. It is set as 1X by default. Click the arrow to display the drop down list of objective lenses.

3.

Move the cursor onto the image window and click and drag the mouse over the area / distance to be measured. mouse on the defined area. Change the measurements by clicking and dragging the

4.

Click the right mouse button on another part of the image to display a shortcut menu. Select Freeze or Lock to save the measurements.

Note: Measurements may be unlocked, but to cancel the freeze status, the Undo
command must be selected.

Erasing a Measurement
To erase a measurement, apply the Undo command before saving an image file.

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Customizing Your Settings

Configuring your measurement settings
1. By clicking the Measure tab on the Control Panel, measurement settings may be customized.

The measurement units and accuracy parameters (how may digits to display after the decimal point) may also be set here. 2. After performing a measurement, click the right mouse button to display a shortcut menu where, by selecting the Unit command, the measurement units may be changed. If the Properties command is selected, a dialog box will appear which allows users to change the measurement data colors and line properties.

Configuring your image and video capture settings

Click the Setting button in the Plus Style or the Setting button Standard Style to display the following dialog boxes: in the

Plus Style

Standard Style

Here, define the size of the images to be captured, how often to capture a frame, and other image and video properties. 20

Adding Sound to Your Files

Note:
To add sound to images, a microphone and a sound card with a microphone input jack are needed, as well, files must be saved in SFC format (default). 1. Select the Music tab on the Control Panel and click the Add Audio to Current Image button. Click the left mouse button on the image and a frame will appear. Click the Record button and the Record dialog box appears. Click the record button on this dialog to begin recording. 2. Click the Stop button to stop recording.

How to Play Sound

1.

Select the Music tab on the Control Panel and click the Playlist button to display the Playlist dialog box. Click the right mouse button and select the Add command to add sound files to the list.

2.

Click the Play button and the sound files will be played in turn.

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How to Combine Images

1. Click the Album button to display the Album window. Next, click the Amalgamation tab to display the corresponding panel.

2.

Click and drag the pictures to be combined into the Amalgamation button panel. and Click select the an Amalgamation

amalgamation method to combine them. The combined picture will then appear in the preview window.

3.

Or, select the pictures to be combined and click the right mouse button. Select the Amalgamation command from the shortcut menu and select a method to combine them. The combined picture will then appear in the preview window.

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Introduction to MCCamera
1) Motic MC1000
1. Before using the Motic MC1000, open the Setting panel and select the MC1000Camera option to be able to run the Motic MC1000. 2. The Exposure and White Balance operations will be carried out automatically to make the quality and color of the image in the preview window similar to those of actual images. The Motic MC1000 window will display as shown on the right:

3. Using the control panel, easily change the quality and effects of the image shown in the preview window.

4. Click the Capture button below:

to capture the real time image shown in the preview

window. After an image has been captured, the work space will display as shown

The captured image will be saved in the Temp folder under the current user.

5. Click the Auto Capture button (next to the Capture button) and the program will capture several images automatically.

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6. Motic MC1000 also enables users to record video. Click the Video button on the Tool Bar, fill in the name and path, and click OK to record and save video.

Recording will begin automatically; click the STOP button to stop recording.

7. Motic MC1000 enables users to adjust the Brightness, Contrast, Saturation, etc. of images. If the brightness is adjusted or the specimen switched during observation, apply the Auto exposure and White Balance commands to adjust the image. 8. The Gain (Color) of images can also be adjusted through these slider bars.

2) Motic MC2000
1. Before using the Motic MC2000, open the Setting panel and select the MC2000Camera option to be able to run the Motic MC2000.

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2. The Exposure and White Balance operations will be carried out automatically to make the quality and color of images in the preview window similar with those of actual images. The Motic MC2000 window will then display as shown on the right:

3. Using the control panel, easily change the quality and effects of the image shown in the preview window.

4. Click the Capture button below:

to capture the real time image shown in the preview

window. After an image has been captured, the work space will display as shown

The captured image will be saved in the Temp folder under the current user.

5. Click the Auto Capture button (next to the Capture button) and the program will capture several images automatically.

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6. Motic MC2000 also enables users to record video. Click the Video button on the Tool Bar, fill in the name and path, and click OK to record and save video.

Recording will begin automatically; click the STOP button to stop recording.

7. Motic MC2000 enables users to adjust the Brightness, Contrast, Saturation, etc. of images. If the brightness is adjusted or the specimen switched during observation, use the Auto exposure and White Balance commands to adjust the image. 8. A ROI function is provided as well. Drag the mouse in the preview window to define a rectangle. Preview the defined region by clicking the ROI button. Click the button again to restore the full image.

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9. Motic MC2000 also provides a Real-time Image Preview function. Click the button on the Tool Bar to be able to select different filters with which to process either the whole image or just the defined region.

10. Motic MC2000 has the ability to capture a clear, sharp image even when there is insufficient illumination.

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Frequently Asked Questions (FAQS)

Q: Ive installed the software, made sure that the viewing lever is pulled out (if applicable) and still no image is produced using the digital microscope. Why? (For Camera 3 users) A: In order to view images, the default video setting must be set to S-Video. To check if your settings are correct: Open the Motic DS Capture window. Select the Options menu.

Select the Video Crossbar option.

Make sure the Input Video Source is set to S-Video and then click the OK button. Q: When I put the CD in the CD-ROM drive, nothing happens; the software does not start automatically. Why? A: The windows auto-insert detection feature may be disabled. To begin software installation: 28

1. Double click the My Computer icon 2. Right click on the CD-ROM drive icon 3. Select the AutoPlay feature and installation should begin. Q: If spots appear in the Motic DS Capture window while trying to capture an image, how do I fix this problem?

A: If spots appear in the Motic DS Capture Window, the

problem probably lies in the resolution settings. When capturing an image, the resolution defined in the Properties Dialog Box should be the same as the resolution defined in the Windows operating system.

For example, if you set the resolution as RGB24, the resolution of the Windows system should be set to either RGB24 or RGB32. If you set the resolution as RGB16, the resolution of the Windows system should be set as RGB16 as well.

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 1999-2004 Motic China Group Co., Ltd. All rights reserved. Motic is a registered trademark and service mark of Motic China Group Co., Ltd. Microsoft, Windows logo are registered trademarks of Microsoft Corporation. All other trademarks are the property of their respective owners.
Design Change: The manufacturer reserves the right to make changes in instrument design in accordance with scientific and mechanical progress, without notice and without obligation. SP010323C 20041216