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AS 2166

One-mark pipettes
Australian Standard™

Laboratory glassware—
AS 2166—2002
 This Australian Standard was prepared by Committee CH-001, Laboratory
Glassware and Related Apparatus. It was approved on behalf of the Council of
Standards Australia on 15 November 2002 and published on 11 December 2002.

The following are represented on Committee CH-001:

Australian Chamber of Commerce and Industry
National Association of Testing Authorities Australia
National Standards Commission
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Royal Australian Chemical Institute

Royal College of Pathologists of Australia
Science Industry Inc.

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This Standard was issued in draft form for comment as DR 01234.

 AS 2166—2002

Australian Standard™
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Laboratory glassware—
One-mark pipettes

Originated as AS R16—1953.
Previous edition AS 2166—1995.
Third edition 2002.

COPYRIGHT
© Standards Australia International
All rights are reserved. No part of this work may be reproduced or copied in any form or by any
means, electronic or mechanical, including photocopying, without the written permission of the
publisher.
Published by Standards Australia International Ltd
GPO Box 5420, Sydney, NSW 2001, Australia
ISBN 0 7337 4928 3
 AS 2166—2002 2

PREFACE
This Standard was prepared by the Standards Australia Committee CH-001, Laboratory
Glassware and Related Apparatus to supersede AS 2166—1995, Laboratory glassware—
One-mark pipettes.
This Standard is based on but not equivalent to ISO 648:1977, Laboratory glassware—One-
mark pipettes (reconfirmed 1999). This edition is essentially the same as the 1995 edition
but has been reformatted for clarity and Clause 7.1 considering delivery time has been re-
written.
The objective of this Standard is to provide a specification for one-mark pipettes required
for general use in laboratories.
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 3 AS 2166—2002

CONTENTS

Page
1 SCOPE ........................................................................................................................4
2 REFERENCED DOCUMENTS ..................................................................................4
3 DEFINITIONS ............................................................................................................4
4 NOMINAL CAPACITIES AND TOLERANCES ON CAPACITIES .........................4
5 CONSTRUCTION ......................................................................................................5
6 GRADUATION LINE.................................................................................................6
7 DELIVERY TIME.......................................................................................................9
8 MARKING ................................................................................................................10
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9 COLOUR CODING ..................................................................................................10

 AS 2166—2002 4

STANDARDS AUSTRALIA

Australian Standard
Laboratory glassware—
One-mark pipettes

1 SCOPE
This Standard sets out requirements for one-mark pipettes, suitable for general laboratory
purposes. Two classes of accuracy are specified, Class A pipettes being of higher accuracy
than Class B pipettes.
NOTE: The method of verification and notes for the use of one-mark pipettes are given in
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AS 2162.1.

2 REFERENCED DOCUMENTS
The following documents are referred to in this Standard:
AS
2162 Verification and use of volumetric apparatus
2162.1 Part 1: General—Volumetric glassware
ISO
1769 Laboratory glassware—Pipettes—Colour coding

3 DEFINITIONS
For the purpose of this Standard, the definitions below apply.
3.1 Capacity
The volume of water at 20°C, expressed in millilitres, delivered by the pipette at 20°C,
when it is filled to the graduation line and emptied in accordance with the method given in
AS 2162.1.
3.2 Delivery time
The duration of descent of the water meniscus from the graduation line to the point at which
it appears to come to rest in the jet.
3.3 Waiting time
The time allowed, after the delivery time, to ensure that the total amount of liquid for which
the pipette is calibrated has been delivered.

4 NOMINAL CAPACITIES AND TOLERANCES ON CAPACITIES

The nominal capacities and tolerances on the capacities of one-mark pipettes shall be as
specified in Table 1.

 Standards Australia www.standards.com.au

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AS 2166-2002, Laboratory glassware - One-mark

pipettes
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