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ATOUROFTHECELL
There is two important
All organisms are made of cells, and a cell by itself is a microcosm. Robert Hooke described cells using a light microscope in 1665. concepts in microscopy, which are magnification and resolving power. Magnification = How much larger an object is made to appear compared to its real size. Resolving power = Minimum distance between tow points that can still be distinguished as two separate points. Abb formula, defining the resolution limit, clearly shows that the resolution of a light microscope is limited by the wavelength of visible light. Maximum possible resolution of a light microscope is 0.2m and the resolving power of electron microscopes is about 0.2nm; which is thousand time higher than light microscope once. The Highest magnification in a light microscope; with maximum resolution, is about 1000 times. R = x .62:nsin = .57m x .62: 1.3 = 0.25 wavelength. nsin = numeric opening is constant = 1.3. According to this formula, the resolving power is inversely related to Instead of light, electron microscopes use electron beams because they have much shorter wavelengths than visible light, this is why we can obtain higher magnification. However, with the EM we can usually only view dead cells because of the elaborate preparation required and may introduce structural artifacts. Sometimes when we are interest by a specific component of cells we have to do what we call cell fractionation. Cell Fractionation = Technique which involves centrifuging disrupted cells at various speeds and periods to isolate components of different sizes, densities and shapes. Development of the ultracentrifuge made this technique possible. Ultracentrifuges can spin as fast as 80.000 rpm, applying a force of 500.000g, but before we proceed to centrifugation we should follow different steps to homogenize the tissue and its cells. Cells are homogenized by ultrasound or grinding, and the resulting cellular soup is separated into component fractions by differential centrifugations. Centrifugation of the homogenate at low or slow speed will give a pellet containing Nuclei and other larger particles, The remaining supernatant is centrifuged at increasing speeds, each time isolating smaller and smaller cellular components in the pellet. You can repeat those steps, increasing time or speed, or even both. Also, sometimes you have to do the centrifugation under low temperature to not denaturate the structures. Each cellular fraction contains a large quantity of the same cellular components, thus permitting the isolated study of their metabolic functions.
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A panoramic view of the cell Living organisms are made of either prokaryotic or eukaryotic cells. This two major kinds of cells, can be distinguished by structural organization.
Prokaryotic
Found only in the kingdom monera. No true nucleus, the DNA is concentrated in a region the nucleoid. No membrane-bound organelles. Other comparison we can make is the size of cells.
Eukaryotic
Found in the other kingdoms. True nucleus; bounded by nuclear envelope. Membrane-bound organelles.
What determine the range of cell size? It is limited by metabolic requirements. The lower limits are probably determined by the smallest size with: Enough DNA to program metabolism. Enough ribosomes, enzymes and cellular components to sustain life and reproduce. The upper limits of size are imposed by the surface area to volume ratio. As a cell increases in size, its volume grows proportionately more than its surface area. The surface area of the plasma membrane must be large enough for the cell volume in order to provide an adequate exchange surface for oxygen, nutrients and wastes, so, this explain the Importance of Compartmental Organization The average eukaryotic cell has a thousand times the volume of the average prokaryotic cell, but only a hundred times the surface area. Eukaryotic cells compensate for the small surface area to volume ratio by having internal membranes . This is a real Partition of the cell into compartments. The nucleus contains a cells genetic library or most of the cells DNA. Nucleus = A generally conspicuous membrane-bound cellular organelle in a eukaryote; contains most of the genes that control the entire cell. Enclosed by a nuclear envelope. Nuclear envelope = A double membrane which encloses the nucleus in a eukaryotic cell. Is two lipid bilayer membranes separated by a space of about 20 to 40 nm. The inner membrane is lined by the nuclear lamina, a layer of protein filaments that helps to maintain or stabilizes nuclear shape. The nucleus contains most of the cells DNA which is organized with proteins into a complex called chromatin, the substance of chromosomes.
Nucleolar organizers = Specialized regions of some chromosomes, with multiple copies of genes for rRNA (ribosomal RNA) synthesis.
The nucleus controls protein synthesis in the cytoplasm via: Messenger RNA (mRNA), transcribed in the nucleus from DNA instructions.
The endoplasmic reticulum manufactures membranes and performs many other biosynthetic functions.
Endoplasmic reticulum (ER) is the most extensive portion of endomembrane system. It is continuous with the outer membrane of the nuclear envelope and encloses a network of interconnected tubules or compartments called cisternae.
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The Golgi apparatus finishes, sorts, and ships many products of the cell
The Golgi apparatus consists of a stack of flattened membranous sacs. Vesicles that bud from the ER join to the cis face of the Golgi apparatus, adding to it their contents and membrane. Products that travel through the Golgi apparatus are usually modified or refined as they move from one cisterna to the next. Some polysaccharides are manufactured by the Golgi. Golgi products are sorted into vesicles, which pinch off from the trans face of the Golgi apparatus. These vesicles may have surface molecules
R + H2O2
2H2O2
catalase
2H2O + O2
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Current thought is that peroxisome biogenesis occurs by pinching off from preexisting peroxisomes. Necessary lipids and enzymes are imported from the cytosol. A Summary of Relationships Among Endomembranes. Components of the endomembrane system are related through direct contact or through vesicles.
is an extension of is confluent with Smooth ER
Nuclear Envelope
Rough ER
membrane and secretory proteins produced in ER are transported in Vesicles fuse with the forming face of Golgi Apparatus pinches off maturing face Vesicles give rise to Lysosomes and Vacuoles fuse with and add to plasma membrane and may release cellular products to outside Plasma Membrane
Mitochondria:
Two membranes, each a phospholipid bilayer with unique embedded proteins, enclose a mitochondrion. A narrow intermembrane space exists between the smooth outer membrane and the convoluted inner membrane. The folds of the inner membrane, called cristae, create a large membrane surface area and enclose the mitochondrial matrix. Within this matrix are enzymes that control many of the metabolic steps of cellular respiration. Other important enzymes are built into the inner membrane. The inner and outer membranes divide the mitochondrion into two internal compartments: Intermembrane Space and Mitochondrial Matrix. Intermembrane Space is a narrow region between the inner and outer mitochondrial membranes. Reflects the solute composition of the cytosol, because the outer membrane is permeable to small solute molecules. Mitochondrial Matrix is a compartment enclosed by the inner mitochondrial
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membrane.
respiration. Some enzymes of respiration and ATP production are actually embedded in the inner membrane.
Chloroplasts:
Plastids are plant and algal membrane-bound organelles that include amyloplasts, which store starch; chromoplasts, which contain pigments; and chloroplasts, which contain the green pigment chlorophyll and function in photosynthesis. Found in eukaryotic algae, leaves and other green plant organs. Are lens-shaped and measure about 2mm by 5 mm. Are dynamic structures that change shape, move and divide. Chloroplasts are divided into three functional compartments by a system of membranes. Intermembrane Space. The chloroplast is bounded by tow membranes or by a double membrane which partitions its contents from the cytosol. space separates the two membranes. A narrow intermembrane
Thylakoid Space.
Thylakoids form another membranous system within the chloroplast. The thylakoid membrane segregates the interior of the chloroplast into two compartments: thylakoid space and stroma. to sugar occur in the stroma. Stroma is a viscous fluid outside the thylakoids. Photosynthetic reactions that use chemical energy to convert carbon dioxide Thylakoids, which enclose the thylakoid space, are Thylakoids function in the steps of flattened membranous sacs inside the chloroplast.
photosynthesis that initially convert light energy to chemical energy. Some thylakoids may be stacked together to form structures called grana.
Microtubules:
All eukaryotic cells have microtubules, which are hollow rods constructed of two kinds
supporting framework of the cell, microbubules serve as tracks along which organelles move with the aid of motor molecules. Separation of chromosomes during cell division. In many cells, microtubules radiate out from a region near the nucleus called a centrosome. In animal cells, a pair of centrioles, each composed of nine sets of triplet microtubules arranged in a ring, may help to organize microtubule assembly for cell division. Are about 150 nm in diameter and are arranged at right angles to each other. They replicate during cell division and may organize microtubule assembly during cell division, but must not be mandatory for this function since plants lack centrioles.
( 9 + 2 pattern),
plasma membrane. Each doublet is a pair o attached microtubules. One of the pair shares a portion of the others wall. Each doublet is connected to the center of the ring by radial spokes that end near the central microtubules. Each doublet is attached to the neighboring doublet by a pair of side arms. Many pairs of sidearms are evenly spaced along the doublets length. A basal body, structurally identical to a centriole, that anchors the microtubular assembly of cilia and flagella. Basal Body can convert into a centriole and vice versa. May be a template for ordering tubulin into the microtubules of newly forming cilia or flagella. As cilia and flagella continue to grow, new tubulin subunits are added to the tips, rather than to the bases. The sliding of the microtubule doublets past each other occurs as arms, (composed of the motor protein dynein, a large protein motor molecule that changes its conformation in the presence of ATP as an energy source), alternately attach to adjacent doublets, pull down (as the conformation of dynein changes), release, and reattach. flagella or cilia. In conjunction with the radial spokes or other anchoring structural elements, this action-driven by ATP-causes the bending of the
Microfilaments:
(Actin Filaments) Microfilaments are solid rods consisting of a helix of two chains of molecules of the globular protein actin. In muscle cells, thousands of actin filaments
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interdigitate with thicker filaments made of the protein myosin. The sliding of actin and myosin filaments past each other, driven by AP-powered arms extending form the myosin, causes the shortening of the cell and thus the contraction of muscles. Microfilaments seem to be present in all eukaryotic cells. They function in support, such as in the core of microvilli; in localized contractions, such as the pinching apart of animal cells when they divide and such as the extension and retraction of pseudopodia; and in cytoplasmic streaming in plant cells.
Intermediate Filaments:
microtubules and microfilaments and are more diverse in their composition each type is constructed from different protein subunits. Intermediate fibers appear to be important in maintaining cell shape and anchoring certain organelles. The nucleus is securely held in a web of intermediate fibers, and the nuclear lamina lining the inside of the nuclear envelope is composed of intermediate filaments. The various kinds of intermediate filaments may serve as the superstructure of the entire cytoskeleton.
The extracellular matrix (ECM) of animal cells functions in support, adhesion, movement, and development.
Animal cells secrete an extracellular matrix (ECM) composed primarily of glycoproteins. Collagen forms strong fibers that are embedded in a network of proteoglycans. Cells may be attached to the ECM by fibronectins that bind to integrins, receptor proteins that span the plasma membrane and bind to and communicate with microfilaments of the cytoskeleton. The ECM provides migratory paths for some cells in developing embryos. A cells contact with its ECM appears to influence the activity of genes in the nucleus.
Intercellular junctions integrate cells into higher levels of structure and function. Plasmodesmata =
are channels
cytoplasm connect bordering cells and water and small solutes can move. The plasma membranes of adjacent cells are continuous through the channel, linking most cells of a
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There are three main types of intercellular junctions between animal cells.
Tight junctions =
block transport of substances through the intercellulare space. So, they are fusions between adjacent cell membranes that create an impermeable seal across a layer of epithelial cells.
Desmosomes =
but permit substances to pass freely through intracellular spaces. The desmosome is made of: Intercellular glycoprotein filaments that penetrate and attach the plasma membrane of both cells. It is a dense disk inside the plasma membrane that is reinforced by intermediate filaments made of keratin (a strong structural protein). So Desmosomes are strong connections between adjacent cells.
Gap junctions =
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