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Definition of Terms Sanitation- is the hygienic means of promoting health through prevention of huma n contact with the hazards

of waste (http://wikipedia.org/wiki/sanitation). The spread of infection is lowered or even eliminated if proper sanitation is implem ented. Asepsis- is the state of being free from disease causing contaminants refers to those practices used to promote or induce asepsis in an operative field in surge ry, medical to prevent infection (http://wikipedia.org/wiki/asepsis). It is a st ate where in microbes are absent in a specific instrument and the occurrence of nosocomial infection is eliminated. Antiseptic- antimicrobial substances that are applied to a living tissue/ skin t o reduce the possibility of infection, sepsis or putrefaction. (http://wikipedia .org/wiki/antiseptic). It is a agent that eliminates infectious pathogen present in a living tissue that may cause infection. Disinfectants- are antimicrobial agents that are applied to non-living objects t o destroy microorganism the process of which is known as disinfection (http://wi kipedia.org/wiki/disinfectants). It is a substance that eliminates microbes pres ent in an instrument this is seldom done before a procedure to ensure the safety of the patient.

Procedure 1. Prepare 3 instruments that will be used in the experiment and prepare th e substance (TUBIG KANAL) that will be used to soil the instruments. 2. Soak the instruments into the basin (TUBIG KANAL) at the same time. 3. Leave the instruments soaked for 2-3hours 4. Remove the instruments from the basin 5. Observe ASEPTIC technique before swabbing the instruments 6. Swab the instruments at the same time and at the same manner. Label it a ccording to the assigned disinfectant for that instrument 7. Properly store the sterile cotton swab used 8. Soak the instruments in their assigned disinfectants at the same time 9. Leave the instruments soaked for 15minutes 10. Remove the instruments from the basin 11. Observe ASEPTIC technique before swabbing the instruments again 12. Swab the instruments at the same time and the same manner. Label it agai n according to the assigned disinfectant used for the that instrument 13. Properly store the sterile cotton swab used 14. Inoculate the samples to different cultures 15. Incubate the culture for 24 hours 16. Count and compare the number of colonies of each culture with the same l abel of disinfectant assigned and compare the number of colonies present 17. Record and evaluate the results of the experiment