Agilent GC/MS Portfolio

32nd ISCC
Riva del Garda
GC/MS Users Meeting

Smallest footprint, single GC column 5975VL with 6850GC

7000A GC/MS/MS

Industry leading GC/MS System 5975C with 7890GC

Page 1

Power of MRM
ISCC User Meeting May, 2008

Agilent 7000A GC/MS/MS

32nd ISCC
Riva del Garda
GC/MS Users Meeting

7000A Width
36 cm/14 inches
5975: 31 cm/12 inches

Platform
2.5 cm/ 1 inches
ISCC User Meeting

Page 2

May, 2008

GC/MS Triple Quad (QQQ)

Carrier Gas (He, H2 ) Collision Gas (Ar, N2, He)

32nd ISCC
Riva del Garda
GC/MS Users Meeting

Ion Source Ionize

Quad 1 Mass Analysis

Quad 2 Collision Cell

Quad 3 Mass Analysis

Detector

ISCC User Meeting Page 3 May, 2008

GC/MS Triple Quad (QQQ)

Carrier Gas (He, H2 ) Collision Gas (Ar, N2, He)

32nd ISCC
Riva del Garda
GC/MS Users Meeting

Ion Source Ionize

Mean Free Path

Quad 1 Mass Analysis

Long

Quad 2 Collision Cell

Quad 3 Mass Analysis

Long

Detector

Short

ISCC User Meeting Page 4 May, 2008

GC/MS Triple Quad (QQQ)

Carrier Gas (He, H2 ) Collision Gas (Ar, N2, He)

32nd ISCC
Riva del Garda
GC/MS Users Meeting

Ion Source Ionize

Quad 1 Mass Analysis

Quad 2 Collision Cell

Q1 Postfilter Q2 Prefilter

Quad 3 Mass Analysis

Detector

ISCC User Meeting Page 5 May, 2008

Agilent 7000 GC/MS/MS (QHQ)

Collision Gas (N2 )

32nd ISCC
Riva del Garda
GC/MS Users Meeting

Ion Source Ionize

Quad 1

Hexapole Collision Cell

Quad 2

Detector

Q1 Postfilter

Q2 Prefilter

The hexapole field has excellent transmission efficiency for precursor and product ions
ISCC User Meeting Page 6 May, 2008

32nd ISCC

What is MRM MS/MS?

Multiple Reaction Monitoring

Riva del Garda

GC/MS Users Meeting

EI: many ions from the source EI-MS/MS

Product 2 Product 1

Q1 SIM

isolate precursor before CID

Product 3

CID + Q2 SIM
ISCC User Meeting Page 7 May, 2008

32nd ISCC

Why MS/MS? Greater Selectivity Than SIM EI-SIM

selectivity proportional to spectral resolution no selectivity against ions with same m/z
interference

Riva del Garda

GC/MS Users Meeting

EI-MS/MS
Precursor selectivity same as SIM
High probability that at least one product ion will be a unique dissociation product of the precursor BUT not the interference
Product 2 Product 1 Product 3
interference

analyte

unit mass resolution

Precursor Ion

The precursor ion should NOT be used for ion ratios or quantitation since the interferences will be the same as the SIM ion
ISCC User Meeting Page 8 May, 2008

Why MS/MS? Lower detection limits by reducing noise EI: many ions from the source

32nd ISCC
Riva del Garda
GC/MS Users Meeting

Product ion signal often decreases, but the percentage decrease in noise is much larger for real samples; S/N and detection limits improve
Product 2 Product 1

Q1 SIM

isolate precursor before CID

Product 3

chemical noise eliminated

CID + Q2 SIM

Product ion measured against zero chemical noise

ISCC User Meeting Page 9 May, 2008

Why a GC/MS/MS System?

32nd ISCC
Riva del Garda
GC/MS Users Meeting

Allows for the selective quantitation of target compounds in high chemical background samples Better S/N in complex matrices than can be achieved by single quadrupole scan or SIM approaches. Newer regulations in some markets specify analytical power commensurate with GC/MS/MS

ISCC User Meeting Page 10 May, 2008

Why GC/MS/MS? A Picture Is Worth a Thousand Words

100 fg HCB

32nd ISCC
Riva del Garda
GC/MS Users Meeting

GC/MS Single Quad SIM

MS SIM

GC/MS/MS QQQ MRM

MS/MS MRM

EI 100fg HCB in DIRTY Matrix

S/N: 116:1 RMS

A chromatographers dream: single peak on flat baseline

ISCC User Meeting Page 11 May, 2008

Why GC/MS/MS? A Picture Is Worth a Thousand Words

100 fg HCB

32nd ISCC
Riva del Garda
GC/MS Users Meeting

GC/MS Single Quad SIM

MS SIM

GC/MS/MS QQQ MRM

MS/MS MRM

EI 100fg HCB in DIRTY Matrix

S/N: 116:1 RMS

A chromatographers dream: single peak on flat baseline

ISCC User Meeting Page 12 May, 2008