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Course teacher,
Introduction
Maize suffers damage from a large number of insect Insects are a particularly acute problem in tropical regions. Genetic differences in the host plant response of maize varieties through biochemical and biophysical basis of resistance against insect Transgenic maize
Resistance?
Resistance is a relative property, based on the comparative reaction
of resistant and susceptible plants, grown under similar conditions, to the pest insect.
I. Allelochemical
Benzoxazinoids
Benzoxazinoids
Natural benzoxazinoids were discovered in 1960 in rye when resistance against fungi was investigated. It is predominantly found in genera of the Gramineae. In maize, it is the methoxy derivative, DIMBOA that present. DIMBOA-glucoside content in corn seedlings can reach
DIMBOA
is
an
enzyme
inhibitor
of
chymotrypsin,
aphid
(McMullen et al.,2009)
zea).
This finding provides support for the assumption that maize RIP plays a role in resistance to maize-feeding insects.
(McMullen et al.,2009)
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Insect(s) affected
Southwestern corn borer, Fall armyworm European corn borer Fall armyworm Southwestern corn borer
Thick cuticle
Low trichome density Silica Tight husks
Sugarcane borer
Corn earworm European corn borer Corn earworm, Maize weevil
(CIMMYT,2005) 13
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Transformation Technology
For maize transformation ballistic and protoplast transformation methods used successful recovery of fertile transformed maize plants.
In the protoplast method, the cell walls are first removed. Then the cell
membrane becomes readily permeable to foreign DNA. Movement of foreign DNA through the cell membrane is facilitated either by applying an electrical current (electroporation) or adding Polyethylene Glycol (PEG).
Foreign DNA in solution surrounding the cells passes through the cell membrane, with some of it being incorporated into the nuclei of cells.
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Thank You
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