Académique Documents
Professionnel Documents
Culture Documents
AND
CROP
IMPROVEMENT
Genomics and crop improvement
Genome contains total genetic information
carried by a single set of chromosomes in
a haploid nucleus
It is the unit of information transmission
(DNA replication) whereas genes in an unit
of expression
Genomics is the study of genes and their
role in the structure, growth and
development, quality, health and diseases
of plants
Application of Genomics
Gene structure
Gene identification and cloning
Gene prediction/ discovery
Genetic mapping and locating genes
Genome projects
Genomic database
Genome manipulation (Mol. Biology)
QTLs
Molecular markers and MAS
Diagnostics on the basis of transcription to RNA
and translation to protein
Study of Genomics
Karyotype analysis/ chromosome profile
Molecular cytogenetics
Molecular dissection
DNA studies & DNA sequencing
Gene discovery
Search for new genes
Exon Prediction
Comparative genomics & orthologous loci
Gene expression and traits
Genome projects
Comparative genomics
Gene banks and chromosome stocks
Expression (mRNA) profiles and
responses and interactions
Data bases and Networking
Genome projects
Crop improvement
Plant Genomic Strategies
Diversity Genomics
Applied Genomics
Genome Vision
Structural Genomics
Comparative Genomics
Functional Genomics
Nutritional Genomics
Computational Genomics
Integrative Bioinformatics plus K mgt
Cell cycle / Mitosis
Life cycle of a cell marked by cell
division. The four phases are:
G1 (Gap 1)
S (Synthesis) DNA replication’
G2 (Gap 2)
M (Mitotic phase) Chromosome
separation
Replication in a short time with precision
Somatic cells
WALTHER FLEMMING (1882)
Describes “
MITOSIS”
DESCRIBES STAGES OF MEIOSIS
Crossing Over
Analogous Homologous
Orthologous Paralogous
PARALOGUES ORTHOLOGUES
Genes within the same genome Genes that share the same
that share an ancient gene ancestral gene performing same
performing diverse biological biological function in different
functions species but have diverged in
sequence due to selective
evolution
Diversity genomics
Sequence polymorphism
Heritable phenotypic differences
Genetic diversity
Novel molecular markers/traits
Linkage disequilibrium in natural and
domesticated crop plants
Selection sweeps in genomics regions
Introgression scales
Mechanisms of crop diversity
Functional Genomics
Functional Genomics
Genome sequence
Gene identification
Gene trapping
Mutagenesis
Genetics manipulation
SY ST EMS VIEW OF C EN TRAL
DOGMA
CE NTRAL DOGM A OF
MO LECUL AR BI OLOGY
GENE S TRU CTU RE
GE NE EX PR ESS IO N
GE NE EX PR ESS IO N
MOLECULES PARTICIPATING IN
INFORMATION FLOW AND THE
FUNCTIONAL SITES
MOL EC UL PRO CE SSI NG FUN CT IO NAL SI TE S INT ER AC TIN G
E MOL EC UL ES
DNA REP LI CAT IO N REP LI CA TIO N O RI GIN ORI GI N
TRA NS CRI PT ION PRO MO TO R REC OG NI TIO N
COM PL EX
ENH AN CE R
OPE RA TO R RNA P OL YME RAS E
OTH ER P ROK ARY OT IC TRA NS CR IPT ION
REG UL AT ORS FAC TO R
REP RE SS OR etc
RNA POS T- SPL IC E SIT E SPL IC EO SOM E
TRA NS CRI PT ION
AL PR OCE SS ING TRA NS LA TIO N RIB OS OM E
TRA NS LAT IO N INI TI AT ION SI TE
CONT.. MOLEC ULES PARTI CIPAT ING IN
INFO RMAT ION FL OW AN D TH E
FUNC TION AL SIT ES
MOL EC UL PRO CE SS ING FUN CT ION AL SI TE S INT ER ACT ING
E MOL EC ULE S
Gene family
Group of similar or identical genes usually on the same
chromosome, arising by gene duplication some of them
work and some of them are switched off or silenced
(pseudo genes)
Gene(Genome) amplification
More or less specific production of multiple copies
Gene conversion
Process in which one member of a gene family acts as a
blue print for the correction of the other - can result in
either suppression of a new mutation or its lateral
spread in the genome
Genomics – overview of general
functions
Genome sequence gene
identification gene
expression gene function
Gene identification – genomic motifs,
comparative genomics, cDNA & ESTs
database, Protein identification
Gene expression – cDNA expression,
Protein expression
Gene expression
Gene activation
Process in which information in a gene is
used to produce a protein and gene
expression via transcription and
translation to produce protein and hence
phenotype
Gene-phenotype relationship (functions)
understanding the field plot techniques,
stat.procedures, molecular markers,
information techniques and equipments
Gene transfer/ genetic engineering
Chromosome engineering
Exon
Exon prediction and Exon trapping
Exon is the portion of the gene that is
transmitted into the mRNA and is
translated into protein
Exon trapping is the method used to
isolate exons from new DNA. In exon
trapping, an R fragment from a new
DNA sequence is cloned into a cognate
R site in an intron of a cloned gene
Expressed sequence tags
DNA sequences derived by sequencung an
end of a random cDNA clone from a library
of interest. Provides rapid ways of
identifying cDNA of interest based on their
sequence EST is an exon specific
sequence.50-500 bp from a cDNA. It
represents a gene. Large sets of ESTs
opens the door for studying gene
expression on a large scale. ESTs represent
tags of expression for a given cDNA library.
(Rastogi et al ., 2004)
Size of mRNA
Gene size: Gene is sequence of chromosomal
DNA required for production of functional RNA
molecule or functional protein. Range in size from
1.5Kb (globin gene) to 2000 Kb (muscular
distrophy gene)
RNA: It is single stranded. RNAs are mRNA, tRNA,
rRNA
mRNA is about 1/10th of the size of the gene from
which it is transcribed
mRNA levels define state of the cells. 5% mRNA,
15% tRNA and 80% rRNA of total RNA. Other
minor RNAs are involved in splicing and telomere
synthesis
mRNA contd….
mRNA
mRNA is template for protein synthesis
assume double helix or single stranded
structure
does not contain chemically modified bases
tRNA
Function in protein synthesis
Contain chemically modified bases
Single stranded DNA and RNA
Adopt random coil structure
Ribosomes
Subcellular organelle involved in protein
synthesis
Made of large and small subunit and rRNA is
integral part of it
rRNA is single stranded and contains chemical
Markers are many..
RFLP – Restriction Fragment Length
polymorphism
RAPD – Randomly Amplified Polymorphic DNA
VNTRs – Variable Number Tandem Repeats
(sat.DNA, omni sat, microsat)
SSR – Single Sequence Repeats or
Microsatellites
DAF – DNA Amplified Finger Printing
AP-PCR – Arbitarily primed PCR
STS – Sequence Tagged Site
SCAR – Sequence Characterized Amplified
Regions
CAPs – Cleaved Amplified Polymorphic Sequence
TYPES OF MARKERS IN GENETICS
MAPS are different kinds
Chromosome/ cytogenetic map in terms of
Chromosome Banding
Genetic mapping/ linkage maps
cDNA map showing locations of expressed DNA
regions (exons) on the chromosome map
Cosmid contig map showing the order of
overlapping DNA fragments
Macro restriction map – order of distance
between enzyme cutting cleavage site
Highest restriction physical map showing the
complete elucidation of the DNA sequence of
each chromosome in the genome
Physical mapping
MOLECULAR MARKERS AND GENETIC
MARKERS
Quantitative Trait Loci (QTL)
Linkage of trait (Quantitative) to marker
loci i.e. phenotype for the QT and
genotype for the marker loci are scored
and if there are differences between mean
phenotype among the marker genotype
classes, then presence of QTL linked to the
marker is inferred. The association
between quantitative trait variation and
marker segregation pattern is worked out.
It is quantified linkage disequilibrium to
locate and clone gene responsible for QT.
QTL is locus of DNA segments that carry
more genes coding for an agronomic trait
DNA Micro array – Recent
Technology
Measures relative to the number of copies
of a genetic message and thus levels of
gene expression at different stages in
development and in different tissues. It
can even measure poorly expressed genes
It is referred to as Reverse Northern. In
micro array experiment, (array
hybridization) cDNA are spotted onto a
filter and hybridized with a probe made
from mRNA population. Probes are made
by reverse transcribing mRNA into cDNA
Microarray contd………
The amount of hybridization to a given
DNA microarray gives snapshot of mRNA expression
in a genome at a particular time
Can take multiple snapshots to watch changing
patterns of mRNAs over time, space and in response
to stimuli
E.g.: Developmental stages, Different tissues,
Starvation, Disease invasion, etc.
Can give indirect indication about levels of specific
Phenotypes and arrays
Plant Breeding Application
Gene discovery
Molecular breeding – get into genes
Diversity patterns and evaluation
Molecular fingerprinting
Marker Assisted/ Aided selection
Markers and genetic solutions
Marker development
Purity testing develop computational models for
biological functions (for traits of interest/ target
traits)
Manipulate value added traits
QTLs – pyramiding and interactions
Transformation and traits
Biotic and abiotic stresses and diagnosis
GENOME
COMPARISONS
Research Directions
COMPARITIVE GENOMICS OF TOMATO, POTATO AND
PEPPER
Comparative genomics of
grasses
Rice Genome Utilities
Proteomics
PROTEins expressed by genOME is
proteomics (Wilkins, 1995). Genome is
entire set of genes and like that proteome
is inclusive of all proteins produced by a
species. Unlike genome, proteome varies
with time and sample
Proteomics Application
1. Identifying diseases progression
2. Protein markers for diagnostics
3. Information generated in proteomics is
complementary with the Genomics information
Bioinformatics
Bioinformatics is management information
system for molecular biology. It is the
application of information techniques
derived from disciplines such as applied
mathematics, computer science and
statistics to understand and organize the
information associated with the molecules
(molecular biology) and biological
phenomena / functions
It is the mathematical, statistics and
computing methods that aim to solve
biological problems using DNA and amino
acid sequences and related information
Component inter relationships
Cytogenetics
Crop Improvement
Proteomics