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John James Wilson Museum of Zoology, Institute of Biological Sciences, Faculty of Science, University of Malaya johnwilson@um.edu.

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museumzoology.um.edu.my

Bulletin of the Museum of Zoology

Next issue end of April 2013

Biodiversity seminars

Special exhibition

Workshop

Hunt for Vampire Moth

Biodiversity Genomics Lab


With capacity for routine DNA barcoding of all animal groups

DNA barcoding of bats

Funded by UMRG158/12SUS: Butterflies of Peninsula Malaysia Project

Testing the utility of DNA barcoding for rapid and accurate assessment of bat diversity in Malaysia in the absence of formally described species
John James Wilson*, Sing Kong Wah*, Muhammad Rasul Abdullah Halim, Rosli bin Ramli, Rosli bin Hashim, & Mohd Sofian-Azirun Genetics and Molecular Research, in press

How to Rapidly Accelerate Biodiversity Inventory in Regions where Most of the Species are Unknown? Malaysias First DNA Barcoding Bioblitz John James Wilson, Kong Wah Sing, Khairunnisa Syaripuddin ..in preparation

Funded by UMRG158/12SUS: Butterflies of Peninsula Malaysia Project

Time taken for completion of DNA barcoding Bioblitz


Step Specimen collection Specimen sorting DNA extraction PCR set-up PCR PCR check 2nd PCR set-up PCR PCR check DNA sequencing Sequence editing, alignment, upload Analysis of DNA barcodes TOTAL Time taken (hrs) 12 2 0.5 2 3 1.5 2 3 1.5 48 1 1 77.5 (3 days)

Cost of DNA barcoding Bioblitz


Step Specimen collection Specimen sorting DNA extraction PCR Details of consumables/reagents 1.5ml microcentrifuge tubes 96-well plate Xytogen kits (100 preps), Pipette tips PCR Master mix, Primers, ddH20, 0.2ml PCR tube, Pipette tips Agarose, TAE buffer, DNA ladder (0.5l), GelRed DNA stain Cost of consumables/reagents /service per specimen in MYR 0.07 0.14 2.33 0.95 (0.95)

PCR check

1.00 (1.00)*

DNA sequencing TOTAL

21.00 24.49 (26.49)

Building a DNA barcode reference library for the true butterflies (Lepidoptera) of peninsula Malaysia: What about the subspecies?
John-James Wilson1,2, Kong-Wah Sing1, Mohd Sofian-Azirun2
PLoS ONE, in review
Funded by UMRG158/12SUS: Butterflies of Peninsula Malaysia Project

Fieldwork

Mining collections

Fieldwork assistance: Minmay Wong, Mary Wilson, Thary Goh, Sasekumar, Evelyn Leung, Nisa, Linda Funding support: LADA Field study Grant, Asian Biodiversity Centre & LESTARI

Global Malaise Program

Field assistance: Ahmed A/L Uda, Sasekumar, Gary Sing, Minmay Wong, Thary Goh, Aaron Teo

Thankyou museumzoology.um.edu.my