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Contents
1. Introduction to Phosphorescence 2. Jablonski Diagram 3. Factors affecting Phosphorescence 4. Phosphorimeter 5. Construction
6. Working
7. Applications 8. Bibliography
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Phosphorescence
Phosphorescence is the emission of light over seconds, minutes ,hours or even days after the cause responsible for the excitation of the molecules is removed.
Phosphorescence is related to fluorescence in emitting a photon, however, a phosphorescent material does not immediately re-emit the radiation it absorbs. It is the reason behind GLOW-IN-THE-DARK materials.
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JABLONSKI DIAGRAM S2
S1
Energy
Absorption Fluorescence
T1
Inter system crossing Phosphorescence
Ground State
S0
4
S* T
ISC Absorption
Phosphorescence
Internuclear separation, R
5
S3 S2 Energy
S1
T1
V5 V4 V3 V2 V1
V4 V3 V2
V1 V0
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pH
Dissolved oxygen
Solvent Choice of solvents affect the phosphorescence to a great extent. Eg: Atoms containing heavier atoms, reduce fluorescence and increase phosphorescence.
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Phosphorimeter
It is a device which is used to measure and identify compounds exhibiting phosphorescence. It is able to distinguish between the two types of luminescences.
Sample Preparation
The solvent must form a rigid solid at 77K. Solvent must be pure so that there is nil background phosphorescence. Ethanol and EPA (5:5:2 mixture of diethyl ether, isopentane and ethanol) are generally used as solvents.
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Sample Asssembly
Sample tube
Liquid Nitrogen
Silvered
Io Sample assembly consists of: A quartz sample tube. liquid nitrogen . Quartz Dewar Vessel.
Unsilvered
this entire assembly is then placed in sample holder called as phosphoroscope. 4/14/2013
Sample tube
Dewar flask Liquid Nitrogen Phosphorescence Can
Emission Shutter
Excitation Shutter To emission monochromator
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Construction of a phosphorimeter
10
Secondary Filter
Lens
Primary Filter
Xenon lamp
Readout
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Working of a phosphorimeter
11
Applications of phosphorescence
Determination of drugs present in blood and urine sample
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Bibliography
1. Physical chemistry ( 9th edition ) by Peter Atkins, Pg. 505-506. 2. Principles of Physical Chemistry by Puri , Sharma and Pathatnia, Pg. 1044-1046. 3. An introduction to Physical Chemistry , New Age International Publishers, Ishwar Das , Archana Sharma, Pg. 157-159. 4. Photoluminiscence of Solutions , Elsevier Publications, Pg. 1-50. 5. www.slideshare.net/HomeAyesha/phosphorimetry 6. www.chemchel.com/applications/applications.html 7. Lott P., Journal Of Chemical Education, Vol. 5, no. 6, June 1974. 8. Lott P., Journal Of Chemical Education, Vol. 5, no. 7, July 1974.
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Thank You!
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