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Taken in part from a presentation by Sheila Cowart &Linda Cummins, Physical Aspects of Forensic Science, Spring, 2001.
Mineral
Asbestos
Manmade
Acetate, rayon, nylon, acrylic, polyester, and olefin
Animal Fibers
Woolen fibers occupy less than 1% of all fibers used in production of textile materials Wool has a microscopic structure that is characteristic of hair The cuticle (outer covering) is made of flattened cells, commonly called scales
Vegetable Fibers
Only cotton is found in any large extent in items of clothing Approximately 24% of total US textile fiber production was cotton in 1979 Other plant fibers, such as jute and sisal, are seen in various types of cordage and baggings
Household Dust
Mineral Fibers
Asbestos fibers are the only natural fibers found in the mineral category Seldom used in items of clothing or household objects, they are rarely found in either the composition of or the debris from items seen in crime labs
Manmade Fibers
Represent approximately 75% of total textile fiber production in US Can be defined as a fiber of a particular chemical composition that has been manufactured into a particular shape and size, contains a certain amount of various additives, and has been processed in a particular way
Perpetrators of crimes are not always aware or able to control the fibers they have left behind or picked up
Important to Remember:
It is important to collect evidence from both complainants and suspects as soon as possible Studies show that some 80% of fibers can be expected to be lost in four hours, with just 5-10% remaining at the end of 24 hours
Methods of Examination
In the recent past, the ID and comparison of fibers were at a relatively simple level which relied heavily on microscopy
Microscopy
Microscopic examination provides the quickest, most accurate, and least destructive means of determining the microscopic characteristics and polymer type of textile fibers.
Microscopic View
Acetate
Dacron
Stereomicroscope
Should be used first to examine fibers. Physical features such as crimp, length, color, relative diameter, luster, apparent cross section, damage, and adhering debris should be noted. Fibers are then tentatively classified into broad groups such as synthetic, natural, or inorganic.
Comparison Microscope
If all of the characteristics are the same under the stereoscope, then the comparison microscope is used. A point-by-point and side-by-side comparison provides the most discriminating method of determining if two or more fibers are consistent with originating from the same source.
Comparison Microscopy
Side-by-side Comparison Bright Field Adjustment
Comparison Microscopy
Characterization Fluorescence
Chemical factors Environmental factors
Comparison Microscope
Comparisons should be made under the same illumination conditions at the same magnifications. This requires color balancing the light sources. A balanced neutral background color is optimal.
Fluorescence Microscopy
The sample is illuminated by ultraviolet light, causing some phases to fluoresce so they can be observed, counted, sized and mapped.
Microspectrophotometry
To the unaided eye, 2 dyes may be identical. Using a grating spectrometer, light absorbed by or reflected from a sample is separated into its component wavelengths, and intensity at each wavelength plotted.
Microspectrophotometry
Microscope linked to a Spectrophotometer
IR Absorption spectrum UV/VIS Absorption Spectrum
Microspectrophotometry
IR spectography identifies generic subtypes indistinguishable by microscopic exam Use of IR microscopes coupled with Fourier transform infrared (FT-IR) spectrometers has greatly simplified the IR analysis of single fibers
Microspectrophotometry
Advantages
Nondestructive Not limited to sample size
Disadvantages
Reactive dyes Chemical composition Tentative identification
Thin-Layer Chromatography
An inexpensive, simple, well-documented technique that can be used (under certain conditions) to complement the use of visible spectroscopy in comparisons of fiber colorants. Dye components are separated by their differential migration caused by a mobile phase flowing through a porous, adsorptive medium.
TLC (continued)
Should be considered for single-fiber comparisons only when it is not possible to discriminate between the fibers of interest using other techniques, such as comparison microscopy (brightfield and fluorescence) and microspectrophotometry in the visible range
TLC (continued)
Technique
Extraction of dyes Solid stationary phase Liquid moving phase Capillary action Chromatogram
TLC (continued)
Interpretation
Rf (retention factor) Color Proportions Scanning densitometer
peak height ratios
Fluorescence
TLC (continued)
Analysis of Chromatograms
Positive association Exclusion Inconclusive
TLC (continued)
Advantages
Highly discriminatory Inexpensive Dye batch variation Reactive dyes Optically isomeric pair
TLC (continued)
Disadvantages
Destructive Pale fibers Tentative identification
PGC (continued)
In PGC, the fragments generated by pyrolysis are introduced into a gas chromatograph for separation and characterization PGC can be used to ID the generic type of an unknown fiber, and in some cases it can ID subclasses within a generic class
Case Study
On Saturday, September 19,1987, the body of an 18-year-old was found on the side of a road in Finland The deceased was completely naked from the waist down, but her jeans, stockings, and underwear were bundled on her chest
Evidential Value
Establish a sequence of events. Link murder weapon with victim or suspect. Help corroborate victims account. Provide leads about surroundings at time of incident. Link together a number of different victims or criminal activities. Establish a high probability that contact or other association has taken place between people and/or objects.