Forensic Toxicology

Forensic Toxicology
Definition: The science of detecting and identifying the presence of drugs and poisons in body fluids, tissues, and organs.

Controlled Substances Act

Federal Law established 5 schedules of classification of controlled substances based on
Drugs potential for abuse Potential to physical and psychological dependence Medical Value Note: Federal law also controls materials that are used in making drugs and those that are manufactured to resemble drugs

Drug Schedules
Schedule I: Drugs with high potential for abuse and addiction, NO medical value Ex: Heroin, LSD, Ecstasy, Marijuana Schedule II: Drugs with high potential for abuse and addiction, have some medical value with restrictions Ex: PCP, Cocaine, Amphetamines, Most Opiates, Some Barbiturates

Drug Schedules
Schedule III: Drugs with less potential for abuse and addiction, currently acceptable for medical use Ex: Some Barbiturates, Codeine, Steroids Schedule IV: Drugs with low potential for abuse and addiction, currently acceptable for medical use Ex: Tranquilizers like Valium, Xanax, Librium

Drug Schedules
Schedule V: Drugs with low potential abuse, medical use, lowest potential dependency Ex: Some Opiates with Non-Narcotic Ingredients

Role of the Toxicologist

Must identify one of thousands of drugs and poisons Must find nanogram to microgram quantities dissipated throughout the entire body Not always looking for exact chemicals, but metabolites of desired chemicals (ex. heroin morphine within seconds)

Toxicology Procedures
10mL of blood in airtight container
Add anticoagulant Add preservative

2 consecutive urine samples

Some drugs take a while to show up in urine (1-3 days)

Vitreous humor Hair samples

Toxicology Procedures
Screening quick test to narrow down possibilities color tests, TLC, GC, immunoassay

Confirmation determines exact identity GC/Mass Spec Note: TLCthin layer chromatography

Color Tests
Marquis Test:
Turns purple in the presence of Heroin, morphine, opium Turns orange-brown in presence of Amphetamines

Scott Test: Three solutions

Blue then pink then back to blue in the presence of Cocaine

Duquenois-Levine:
Test for marijuana turns purple

More Analytical Tests

Microcrystalline Tests: Identifies drug by using chemicals that reacts to produce characteristic crystals Chromatography: TLC, HPLC and gas separate drugs/tentative ID Mass Spectrometry: chemical fingerprint no two drugs fragment the same

Why?
Think of all the people that you have heard do drugs. US drug manufacturers produce enough barbiturates and tranquilizers each year to give every person in the US 40 pills (thats about 12 billion pills) 18,000 out of 44,000 annual traffic deaths are alcohol related and send over 2 million people to the hospital

Toxicology of Alcohol
Alcohol is absorbed through the stomach and intestine Once absorbed, alcohol is:
Oxidized- in liver by alcohol dehydrogenaseturned into acidic acid Excreted- by breath, perspiration, and kidneysturned into carbon dioxide and water

Factors that Affect Alcohol Absorption

Time of consumption Type of alcoholic beverage Presence of food in stomach

Toxicology of Alcohol
Alcohol intoxication depends on
Amount of alcohol consumed Time of consumption Body weight Rate of alcohol absorption

Fate of Alcohol
Alcohol is absorbed into the bloodstream Distributed through-out the bodys water And finally eliminated by oxidation and excretion

Fate of Alcohol Cont

Note: A. Oxidation is the combination of oxygen and alcohol to produce new products by the liver B. Elimination is removing alcohol from the body in an unchanged state; normally excreted in breath and urine

Alcohol in the Circulatory System

Measuring the quantity of alcohol in the blood system determines the degree to which someone is drunk Two methods of making this measurement
Measurement of alcohol content in blood Measurement of alcohol in breath

Circulation and Alcohol

Circulation and Alcohol

Circulation Definitions
Arterya blood vessel that carries blood away from the heart Veina blood vessel that transports blood toward the heart Capillarya tiny blood vesselwalls exchange materials between blood and tissues Alveolismall sacs in lungsexchange vapors between breath and blood

Circulation Cont
Note: If alcohol is present, it will be passed from the blood into the alveoli where it will be passed on to the mouth and nose during the act of breathing. Evidence has shown that the ratio of alcohol to alveoli air is approx. 2100 to 1This is a basis for relating breath to blood-alcohol concentration.

Analysis of BAC
Breath Tests

Field Sobriety Tests

Blood Tests

Breath Tests
A breath test reflects the alcohol concentration in the pulmonary artery. One instrument used for breath tests is called The Breathalyzer. The Breathalyzer is a device for collecting and measuring the alcohol content of alveolar breath.

The Breathalyzer

The Breathalyzer Cont

The Breathalyzer traps 1/40 of 2100 milliliters of alveolar breath. Since the amount of alcohol in 2100 milliliters of breath approximates the amount of alcohol in 1 milliliter of bloodthe Breathalyzer in essence measures the alcohol concentration present in 1/40 of a milliliter of blood.

Breathalyzer Cont
Once the alveolar breath is trapped it is allowed to undergo a chemical reaction:
2K2Cr2O7 + 3C2H5OH + 8H2SO4 2Cr2(SO4)3 + 2K2SO4 + 3CH3COOH + 11H2O
Potassium dichromate Ethyl alcohol Sulfuric acid Chromium sulfate Potassium sulfate Acetic acid

Dihydrogen oxide

The Breathalyzer indirectly determines the quantity of alcohol consumed by measuring the absorption of light by potassium chromate before and after its reaction with alcohol, using the principle of spectrophotometry

Other Breath Tests

Infrared breath-testing instrument Fuel cell Note: These instruments are used more recently because they dont depend upon chemical reagents and are entirely automated.

Infrared-Breath Test
Uses the principle that infrared light is absorbed when shined on alcohol Essentially, the infrared light passes through a chamber where it will interact with the alcohol and cause the light density to decrease. The decrease in light intensity is proportional to the concentration of alcohol present in the captured breath

Fuel CellBreath Test

A fuel cell converts a fuel and an oxidant into an electrical current. In this test, the breath alcohol is the fuel and atmospheric oxygen acts as the oxidant. Alcohol is converted, generating a current that is proportional to the quantity of alcohol present in the breath.

Infrared and Fuel Cell Breath Tests

Infrared Breath Test uses infrared wavelengths to test for alcohol or other interferences in the breath

Fuel Cell Test converts fuel (alcohol) and oxygen into a measurable electric current

Field Sobriety Testing

Two reasons for the field sobriety test:
1. Used as a preliminary test to ascertain the degree of the suspects physical impairment 2. To see whether or not an evidential test is justified.

Field Sobriety Testing Methods

Field sobriety testing consists of a series of psychophysical tests and a preliminary breath test (typically done with a handheld fuel cell tester) These tests are preliminary and nonevidential in naturethey only serve to establish probable cause requiring a more thorough breath or blood test.

Field Sobriety Tests

Horizontal Gaze Nystagmus
Involuntary eye jerk as eye moves horizontally

Walk and Turn (divided attention tasks) One-Leg Stand

Parts of the brain affected by Alcohol

Alcohol 1st affects the forebrain and moves backward Last affected is medulla oblongata

Alcohol and the Law

1939-1964: intoxicated = 0.15% BAC 1965: intoxicated = 0.10% BAC 2003: intoxicated = 0.08% BAC

At least we dont live in France, Germany, Ireland, or Japan (0.05%) or especially Sweden (0.02%)!

Alcohol and the Law

Try the drink wheel: http://www.intox.com/wheel/drinkwheel.asp

The End