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Wendy
Apr, 2008
Main Content
Preface Basic parameter setting Reagent setting Calibration and QC setting
Preface
Here we set UREA as the example for all Fixed-time items on
UREA
UREA
No. will be generated automatically by the system, eg 1 for the first one.
UREA
Full Name of the item, can be blank if the user dont want to input anything.
UREA
Standard No. of the item, can be blank or involved with the bar code.
UREA
Reac. Type of the item, can be referred from the reagent insert as ...
UREA
Fixed-time
UREA
Fixed-time
Pri. Wave & Sec. Wave should be selected as reagent insert indicates
So we can set it as 340 nm for Pri. Wave; since no secondary wavelength is mentioned, Sec. Wave could be blank.
UREA
Fixed-time 340
UREA
Fixed-time 340
to-be-consumed reactant, which will be less and less during testing, thus, indicating the absorbance is descending in the measurement.
UREA
UREA
Reac. Time & Incuba. Time for the reaction, refer to ...
Preheating time
Incubation time
Reaction time
Fixed 14 cycles
Mono reagent items' reaction time begins when Sample is dispensed. Dual reagent items reaction time begins when R2 is dispensed. The time between S and R2 dispensing is called Incubation time, only dual reagent measurements have incubation time.
2
3 4 5
8
12 16 20
According to the instruction and the minute-cycle conversion table: The incubation time is be 1 minute, namely 4 cycles; The reaction time should be 30 seconds and 30+60 seconds, namely 2 and 6 cycles.
UREA
2
4
UREA
2
4
According to the insert, we can set the Unit as mg/dl or mmol/l for serum testing, and g/24h or mmol/24h for urine samples.
UREA
2
4
mg/dl
UREA
2
4
mg/dl
If the unit is set as mg/dl, its normal to report results without decimals. We can set the Precision as 0.
UREA
2
4
mg/dl
0
UREA
2
4
mg/dl
0
To process with the sample testing, we need 10ul sample, 1000ul R1 and 250ul R2 (namely, the ratio of S/R1/R2 is 1/100/25); According to the volume range of BS-200 (sample: 3-45ul, R1: 180-450ul; R2: 30-450ul), and the indicated S/R1/R2 ratio; We can set Sample volume as 3ul, R1 as 300ul, and R2 as 75ul.
UREA
300
75
3
2
4
mg/dl
0
UREA
300
75
3
2
4
mg/dl
0
Absorbance of R1 Blank (mono rgt.) and Mixed Rgt. Blank (dual rgt.), set according to experience or reagent insert; if no idea, can be blank.
UREA
300
75
3
2
4
mg/dl
0
From the part of Linearity, we can get aware of that, the upper limit of Linearity Range is 300 mg/dl, or 50 mmol/l.
UREA
300
75
3 Fixed-time 340 Descending 300
2
4
mg/dl
0
UREA
300
75
3
2
4
mg/dl
0
Linearity Limit, Substrate Limit and Factor should be set for the Kinetics tests, so here we can leave them as blank.
UREA
300
75
3
2
4
mg/dl
0
Prozone check is set for the turbidimetric tests, which can also be blank.
Thank you!
NOTE: Fixed-time items should be set in the same way as UREA.