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BS-200 Parameter Setting (Fixed-time)

Wendy
Apr, 2008

Main Content
Preface Basic parameter setting Reagent setting Calibration and QC setting

Preface
Here we set UREA as the example for all Fixed-time items on

Generally, Fixed-time item includes: UREA, Cre,

Basic parameter for UREA


Read the reagent insert first, eg as below.

UREA

Input the item name for Test.

UREA

No. will be generated automatically by the system, eg 1 for the first one.

UREA

Full Name of the item, can be blank if the user dont want to input anything.

UREA

Standard No. of the item, can be blank or involved with the bar code.

UREA

Reac. Type of the item, can be referred from the reagent insert as ...

Basic parameter for UREA

Its another saying of Fixed-time.

So we can set it as Fixed-time type.

UREA

Fixed-time

Reac. Type is set as Fixed-time as the reagent insert indicates.

UREA

Fixed-time

Pri. Wave & Sec. Wave should be selected as reagent insert indicates

Basic parameter for UREA

So we can set it as 340 nm for Pri. Wave; since no secondary wavelength is mentioned, Sec. Wave could be blank.

UREA

Fixed-time 340

Pri. Wave is selected as 340 nm, Sec. Wave is blank.

UREA

Fixed-time 340

Direction of the absorbance changing during the reaction, refer to ...

Basic parameter for UREA


So we can find its Descending Moreover, From Reaction Principle, we can see NADH
(routine indicator under 340nm) is a

to-be-consumed reactant, which will be less and less during testing, thus, indicating the absorbance is descending in the measurement.

UREA

Fixed-time 340 Descending

Direction is selected as Descending.

UREA

Fixed-time 340 Descending

Reac. Time & Incuba. Time for the reaction, refer to ...

Basic parameter for UREA


R1 Sample

Preheating time

Incubation time

Reaction time

Fixed 14 cycles

To be set from the insert

0 cycle of reaction time

Mono reagent items' reaction time begins when Sample is dispensed. Dual reagent items reaction time begins when R2 is dispensed. The time between S and R2 dispensing is called Incubation time, only dual reagent measurements have incubation time.

Basic parameter for UREA


Time is all calculated into cycles on BS-200, and 1 cycle equals to 16 seconds. Roughly speaking, we change minutes into cycles for Reac. Time and Incuba. Time as below.
minute 0.5 1 cycle 2 4

2
3 4 5

8
12 16 20

Basic parameter for UREA

According to the instruction and the minute-cycle conversion table: The incubation time is be 1 minute, namely 4 cycles; The reaction time should be 30 seconds and 30+60 seconds, namely 2 and 6 cycles.

UREA

Fixed-time 340 Descending

2
4

Reac. Time is set as 2 and 6, Incuba. Time as 4.

UREA

Fixed-time 340 Descending

2
4

Unit of the item, should be set according to the reagent insert

Basic parameter for UREA

According to the insert, we can set the Unit as mg/dl or mmol/l for serum testing, and g/24h or mmol/24h for urine samples.

UREA

Fixed-time 340 Descending

2
4

mg/dl

We can set the Unit as mg/dl.

UREA

Fixed-time 340 Descending

2
4

mg/dl

Precision of the items results, should be referred to the reagent insert

Basic parameter for UREA

If the unit is set as mg/dl, its normal to report results without decimals. We can set the Precision as 0.

UREA

Fixed-time 340 Descending

2
4

mg/dl
0

Precision of the item is set as 0.

UREA

Fixed-time 340 Descending

2
4

mg/dl
0

R1, R2 and Sample Volume of the item, should be referred to

Basic parameter for UREA

To process with the sample testing, we need 10ul sample, 1000ul R1 and 250ul R2 (namely, the ratio of S/R1/R2 is 1/100/25); According to the volume range of BS-200 (sample: 3-45ul, R1: 180-450ul; R2: 30-450ul), and the indicated S/R1/R2 ratio; We can set Sample volume as 3ul, R1 as 300ul, and R2 as 75ul.

UREA

300

75
3

Fixed-time 340 Descending

2
4

mg/dl
0

R1, R2 and Sample Volume are set as 300, 75 and 3.

UREA

300

75
3

Fixed-time 340 Descending

2
4

mg/dl
0

Absorbance of R1 Blank (mono rgt.) and Mixed Rgt. Blank (dual rgt.), set according to experience or reagent insert; if no idea, can be blank.

UREA

300

75
3

Fixed-time 340 Descending

2
4

mg/dl
0

Linearity Range means the reagents measuring range, refer to

Basic parameter for UREA

From the part of Linearity, we can get aware of that, the upper limit of Linearity Range is 300 mg/dl, or 50 mmol/l.

UREA

300

75
3 Fixed-time 340 Descending 300

2
4

mg/dl
0

We can set the upper limit of Linearity Range as 300 mg/dl.

UREA

300

75
3

Fixed-time 340 Descending 300

2
4

mg/dl
0

Linearity Limit, Substrate Limit and Factor should be set for the Kinetics tests, so here we can leave them as blank.

UREA

300

75
3

Fixed-time 340 Descending 300

2
4

mg/dl
0

Prozone check is set for the turbidimetric tests, which can also be blank.

Reagent parameter for UREA


The basic parameters for UREA have been finished
Next, we should set the reagent information as following

Calibration and QC for UREA


Basic reagent information (Position and Bottle Type) has been set up We should set relevant calibration and QC information for the item

(Please refer to the video of Calibration & QC setting)

Thank you!
NOTE: Fixed-time items should be set in the same way as UREA.