ENGLISH FOR

SPECIALIZED CHEMISTRY 3

SPECTROSCOPY
GROUP 7 LOGO

Members of group 7
1
Dinh Thi Minh Nhat

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Ha Cuong Tuyen

Nguyen Thi Doi

Nguyen Thanh Luan

Nguyen Van Sang

Nguyen Thi Thu Sen

Nguyen Linh Tam

Nguyen Thanh Huy

Tran Thi Thu Thao

Contents
1 2 3 4

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Introduction to spectroscopy
Absortion spectrum Emission spectrum Ultraviolet visible and infrared spectrophotometry

Introduction to spectroscopy
What is spectrophotometry ? Spectrophotometry is the quantitative

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measurement of the reflection or transmission

properties of a material as a function of

wavelength.

Introduction to spectroscopy

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Introduction to spectroscopy

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limited major

minor

Introduction to spectroscopy

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Introduction to spectroscopy
One example
Nessler

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Introduction to spectroscopy

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Alkaline solution

Introduction to spectroscopy

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Equal volumes of the sample and standards

Introduction to spectroscopy

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application

Introduction to spectroscopy

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Introduction to spectroscopy

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At the and of 19th century

Introduction to spectroscopy

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transfer

of energy

Interaction of radiation

Introduction to spectroscopy

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Emission

Introduction to spectroscopy

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Absortion spectrum

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Absortion spectrum

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Atoms and molecules absorb light, the incoming energy excites a quantized structure to a higher energy level. The type excitation depends on the wavelength of the light.

Absortion spectrum

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Absortion spectrum

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Absortion spectrum
E total = E electronic + E vibration + E rotation

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Electrons are promoted to higher orbital by

ultraviolet or visible light. Vibrations are excited by infrared light. Rotations are excited by

microwaves

Absortion spectrum
An absortion spectrum is the absortion of light as a function of wavelength. The spectrum of an atom or molecule depends on its energy level structure, and absortion

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spectra are useful for identifying of

compounds.

Absortion spectrum
Measuring the concentration of an absortion species in a sample is accomplished by applying Law the Beer-Lambert Absorbance = A (or Optical Density

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= OD)

compares it to the intensity of light It measures the intensity of light passing before it passes through the sample (I0) through a sample (I)

Absortion spectrum

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Emission spectrum
Emission liminescence
The relationship among the wavelength and the
energy E are: E=

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where c is the speed of light and h is Planck's Constant.

Emission spectrum of hydrogen Emission spectrum of iron

Emission spectrum
Atomic-opical emission

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For atoms excited by a high temperature energy source this light emission is commonly called atomic or optical

Emission spectrum
Atomic fluorescence
For atoms excited with light it is called atomic fluorescence

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Emission spectrum
Fluorescence- phosphorescence

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Eneration of luminescence through excitation of a molecule which is formally

divided into two categories, fluorescence and phosphorescence.

If the transition is between states of the same spin, it will call fluorescence. If the transition is between states of different spin, it will call phosphorescence.

Emission spectrum
Applies

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At low concentrations the emission intensity will generally be proportional to the concentration of the emission The emission spectrum can be used to determine the composition of a material

Ultraviolet visible and infrared spectrophotometry

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Ultraviolet visible and infrared spectrophotometry Ranges radiation

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1000 760 nm

760 400 nm

400 100 nm

Ultraviolet visible and infrared spectrophotometry

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Source of lights radiation IR Infrared

radiation that can be felt as heat Visible light that you see

vis
sunlight

Ultraviolet radiation that cant be seen or left

uv

Ultraviolet visible spectrophotometry

The most common spectrophotometers are used in the UV and visible regions of the spectrum. They take readings in the region of every 520 nanometers along the visible

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region.

Ultraviolet visible spectrophotometry

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Ultraviolet visible spectrophotometry

Ultraviolet Lights

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Ultraviolet visible spectrophotometry

Visible Lights

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Infrared spectrophotometry

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IR

Thermocouples for infrared radiation

Infrared radiation was discovered 1800

Absorbed infrared radiation causes rotational changes in molecules

Infrared spectrophotometry

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References
city University of Industry, 2012.

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[1]. Le Thi Thanh Huong, English for specialized chemistry, Ho Chi Minh

www.themegallery.com

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