Académique Documents
Professionnel Documents
Culture Documents
Medical Assisting
Third Edition
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Learning Outcomes
46.1 Define microbiology.
46.2 Describe how microorganisms cause disease. 46.3 Describe how microorganisms are classified and named.
46.4 Explain how viruses, bacteria, protozoans, fungi, and parasites differ and give examples of each.
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Introduction
Medical assistant
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Microbiology study of microorganisms (simple forms of life visible only with a microscope)
Microorganisms
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Medical assistant
Assists physician Obtains specimens Prepares specimens for direct examination Prepares specimens for transportation to reference laboratory If office has a POL, performs microbiologic procedures
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Use nutrients needed by cells and tissues Damage cells directly Produce toxins
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Localized symptoms
Generalized symptoms
Normal flora
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microbiology? ANSWER: The medical assistant may assist the physician in obtaining specimens, obtain specimens herself, prepare specimens for direct examination or transport to a reference laboratory, and possibly perform microbiologic procedures. 2. How do microorganisms cause disease?
ANSWER: Organisms cause disease by using nutrients needed by cells and tissues, damaging cells directly, or producing toxins.
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Classification by structure
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Standardized naming
Genus
Species of organism
Represents a distinct type of microorganisms Examples Staphylococcus aureus and Staphylococcus epidermidis
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Viruses
Have only nucleic acid surrounded by a protein coat Must live and grow in living cells of other organisms
Hepatitis virus
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Viruses (cont.)
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Bacteria
Shape Ability to retain dyes Ability to grow with / without air Biochemical reactions
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Shape
Bacillus rod-shaped
Spirillum spiral-shaped Virbrio comma-shaped
Spirillum bacterial classification
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Aerobes grow best in the presence of oxygen Anaerobes grow best in the absence of oxygen
Biochemical reactions
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Special groups
Chlamydiae
Mycobacteria bacilli with a cell wall that differs from most bacteria Rickettsiae
Cell wall structure differs from other bacteria Live and grow within other living cells
Very small Live and grow within other living organisms such as mites and ticks
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Protozoans
Illnesses
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Fungi
Superficial infections
Molds
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Multicellular Parasites
Parasitic insects
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2.
Obtain specimen(s)
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Wet mount Smear Culture medium contains nutrients Examine culture visually and microscopically
4.
Culture specimen
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6.
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Super!
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Specimen Collection
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Devices
Use appropriate collection device or specimen container Sterile swabs absorbent material on the tip
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Swab back of throat in the area of the tonsils Avoid touching any structures in the mouth Prepare culture plate or prepare correctly for transport to laboratory
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Urine specimen
Sputum specimen
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Wound specimen
Stool Specimens
Technique varies
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Follow proper collection procedures and proper collection device Prevent deterioration of specimen Protect anyone handling specimen
2009 The McGraw-Hill Companies, Inc. All rights reserved
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Impressive!
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NaCl mixed with specimen of glass slide Presence of pathogen and movement of microorganism
Used if a fungal infection of the skin, nails, or hair is suspected KOH dissolves keratin that can mask presence of a fungus
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Grams stain
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More common to send specimens for culture to outside labs Culturing involves placing a sample of specimen on a culture medium
Medium nutrients Place in incubator for growth colony develops as microorganism multiplies
2009 The McGraw-Hill Companies, Inc. All rights reserved
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examination by the physician? ANSWER: They are wet mount and KOH mount.
2. How does the examination of stained specimens
facilitate patient care? ANSWER: Stained specimens enable the physician to provide a quick, tentative diagnosis and differentiate between types of infections.
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ANSWER: The culture medium is inoculated with the specimen and placed in an incubator to promote growth of the organism on the culture medium.
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Culture media
Rapid urine culture Uricult Also available for throat, vaginal, and blood specimens
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Transfer some of the specimen onto a culture plate Label the plate correctly Qualitative analysis determination of type of pathogen Quantitative analysis number of bacteria present in sample
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35 to 37 C for 24 to 78 hours Agar side up Requires skill and practice Characteristics of colonies Relative number Changes to media around colonies
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Interpreting cultures
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Procedure
Filter paper containing antimicrobial agents placed on inoculated agar plate Incubated for 24 hours Evaluate effectiveness of agent
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bacteria that said it was resistant to an antimicrobial. What does this mean?
ANSWER: It means that the bacteria was not killed by the antimicrobial and that there was an overgrowth of the bacteria.
2009 The McGraw-Hill Companies, Inc. All rights reserved
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Routine evaluation
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Very Good!
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In Summary
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End of Chapter
Each organism's environment, for the most part, consists of other organisms.
~ Kevin Kelly