Precise Results Accurate Diagnosis

Typical IHC/ISH Work Flow

Pre-Treatment High-temperature heating and rehydration recovers tissue antigenicity Antibody Incubation Primary antibody selectively binds to proteins of interest on cells for accurate diagnosis

Sample Preparation
Deparafinization Rehydration

Coverslip

Protects tissue from scratches & improves optical quality

Permanent Mounting Applies clear adhesive mounting medium to slide

Substrate Chromogen addition results in detection of signal

Detection System Enhance the sensitivity and allow for signal amplification

Approach to Automation*

Critical steps in manual procedure include

Reagent application Incubation Time Incubation Temperature Wash Drying

Accessible to Automation

These can affect the quality of staining

*Ref Approach to automation in IHC, Moreau et al Clin Chim Acta. 1998 Dec;278(2):177-84.

Molecular Pathology Automated

Sample Preparation
Deparafinization Rehydration Pre-Treatment High-temperature heating and rehydration recovers tissue antigenicity Antibody Incubation Primary antibody selectively binds to proteins of interest on cells for accurate diagnosis

Detection System Enhance the sensitivity and allow for signal amplification

Coverslip
Protects tissue from scratches & improves optical quality

Permanent MountingApplies clear adhesive mounting medium to slide

Substrate Chromogen addition results in detection of signal

Automation Ensures Consistent Results

Traceability of Operation
Walk-away operations

Economical use of reagents

Low Cost

Biosafety
Flexibility of Operation

D I A G N O S T I C S

I N F I N I T Y

Microtome to Microscope in

..3 Simple Steps

1
Load Click View

2
Microtome

Microscope

KEY FEATURES
Multi-functional system supports - IHC, ISH and FISH Slide processing options Random (perform template test at any time), Continuous (access new slides on an on-going basis) and STAT [Short Turn Around Time] Multi-format specimen processing Frozen or FFPE Tissues. Cell preparations. Smears and Fine Needle Aspirates. LIS connectivity

KEY FEATURES
Reproducible and Reliable Results
Standardized pre-optimized process from baking through final cover slip. Walk away automation - RFID tagged reagent vials and bar-coded slides (eliminates

mix ups and errors)

Optimized protocols and ready-to-use reagents( no protocol tweaking) Liquid level sensor ensures accurate dispensing and real time reagent tracking (keep track of reagent quantity so runs are not affected due to insufficient reagents)

KEY FEATURES
Adaptable, Efficient, Productive and Cost-Effective Adaptable workflow solution with complete automation. Predictable cost structure (reagent cost per SLIDE can be calculated).

Efficient use of resources, free-up highly skilled technicians for more demanding tasks.
High Throughput - 40 slides in 6 - 8 hour shift (40 at a time). Start delayed run and return to view the completed slides.

KEY FEATURES
Easy to Use

Color coded GUI with real time assay parameters display

Real time audio and visual alerts Color coded bulk reagent carboys Step-by-step slide information log for every slide (can backtrack in case of improper staining result)

ENABLING TECHNOLOGIES
Reaction Micro-chamber with Micro-fluidics Reaction micro-chamber : 25 L to 100 L. Reduced reagent consumption up to 75%. Even distribution of reagents on the specimen. Overnight incubation/ hybridization without drying of specimen.

ENABLING TECHNOLOGIES
eXACTTM Temperature Control on Every Slide

Optimum operating temperature irrespective of laboratory conditions

Temperature range from Ambient to 105C Temperature uniformity across slide +/-2C Active heating and cooling to minimize cross talk Thermocycling: Ambient 95C in <2 min

ENABLING TECHNOLOGIES
RFID and Barcode Enabled Platform Technology RFID tagged reagents vials and bar coded slides Accurate and precise real-time reagent usage tracking.

Liquid Level Sensor and Reagent Dispensing System

Accurate dispensing in micro-volumes Eliminates false negatives by error-free reagent dispensing Redundant automated processes to eliminate human error Disposable pipette tips prevent cross contamination

ENABLING TECHNOLOGIES
On-board Dehydration and Final Cover slip Dehydration and clearing. Permanent Mounting with glass cover slip. Curing and cooling.

LIS Enabled System

Intuitive software designed to adapt any manual protocols. Easy to edit protocols. Reports for inventory management and regulatory compliance.

Why are these features important?

Critical steps in manual procedure include Reagent application. Incubation Time. Incubation Temperature. Wash. Drying. These can affect the quality of staining.

Reaction Micro-chamber with Micro-fluidics.

eXACTTM Temperature Control on Every Slide

Liquid Level Sensor and Reagent Dispensing System. RFID and Barcode Enabled Platform Technology (Record Keeping).

Applications: Technologies for today

IMMUNOHISTOCHEMISTRY

DOUBLE-STAINING

CISH

Applications: Technologies for the Future

Multiplexing Assays of Nucleic Acid and Protein Biomarkers. In situ PCR. Quantitative gene expression profiling. Microarray technologies.

KEY FEATURES
Simultaneous optimization of up to forty parameters in a single run. Co-detection of multiple nucleic acid and protein biomarkers. Multiplexing of single or multiple specimens on a single slide. Multi-format specimen analysis: Tissues - Frozen or FFPE.

Cell preparations.
Smears. Fine Needle Aspirates (FNA).

KEY FEATURES
Intuitive software designed for ease of use and flexibility. Automate any or all steps from baking through final cover slip. Reagent choice Open format or pre-optimized ready to use User Defined Adapts and automates any protocol.

Enabling Technologies
Reaction Micro-chamber with Micro-fluidics Reaction micro-chamber: 10 L to 80 L (Customizable volumes).

Reduces micro-reagent usage up to 90%.

Even distribution of reagents on the specimen. Overnight incubation/hybridization without drying of specimen.

Enabling Technologies
eXACTTM Temperature Control and Thermo-cycling Forty independently thermo-cyclable (PCR) work stations

Thermocycling: Ambient 95C in <2 min

High precision, active heating and cooling on each slide Temperature uniformity across entire slide +/- 2C Temperature range of Ambient to 105C.

Enabling Technologies
On-board Dehydration and Final Cover slip
Eco-friendly (Non-Xylene) dehydration. Permanent mounting and cover slipping.

Curing and cooling.

Ease of Use Real time slide map.

Intelligent bulk fluidics system.

Efficient waste management system. Reports for inventory management and regulatory compliance.

SIMULTANEOUS OPTIMIZATION
An example of the procedure: Baking

Parameters that can be changed: Unlimited addition or deletion of steps Change of reagents and applied volume Change of incubation time

Dewax
Antigen retrieval Superblock Antibody Super enhancer Polymer HRP DAB work solution

Change of times of washes

Change the incubation temperature

Hematoxylin
Clearmount Xmount

Why are these features important?

BioGenex Laboratories Xmatrx system provides two unique capabilities, including the ability to dispense as little as 10 L of reagent and apply cover slips directly onto slides (both of which are designed to conserve ISH probe reagents). - A review of automated slide stainers for IHC and ISH by Joe Myers
http://findarticles.com/p/articles/mi_m3230/is_1_40/ai_n24254665/pg_2/?tag=content;col1

Applications

Co-detection of Nucleic acid and protein Biomarkers

Applications
FISH

Visualization of Circulating Tumor Cells

In Situ PCR

Summary
Diagnostics High Throughput 40 slides in 8 hour Infinity Simultaneous Optimization of 40 assay parameters 10 L 80 L (useful for FISH and microRNA research) Open System

25 L 70 L (pre-optimized quantities)

Closed System

REAGENTS AND CONSUMABLES

IHC- REAGENTS Primary antibodies in RFID tagged vials (for 200 slides) Detection Kits XVizTM Detection System (Standard IHC reagents optimized for automation and BioGenex protocols) SuperSensitiveTM One-step Polymer-HRP Detection Kit

XVizTM Double Staining Polymer Detection Kit

Antigen Retrieval Solutions : EZ-ARTM Elegance Solutions Enzymatic Pre-Treatment Solutions

REAGENTS AND CONSUMABLES

ISH Reagents ISH Probes packaged in RFID vials for staining upto 100 slides Detection Kits XISHTM Detection Kit (Standard ISH reagents optimized for automation and BioGenex

protocols)
XISHTM Double Staining Detection Kit Nucleic Acid Retrieval Solution : increase target nucleic acid accessibility for ISH and eliminate the variability generated by Proteinase K digestion step.

Xmatrx Diagnostics and Infinity Consumables

Barrier Slides ( 18X18mm, 25X25mm for Infinity and 25X40 mm Diagnostics and Infinity) Cover slips OptiMiser Reagent Vials Pipette Tips Reagent Vials RFID tagged reagent volumes

Thank You! Questions? Please keep them coming