Académique Documents
Professionnel Documents
Culture Documents
• Translation
• B cell with IgD and IgM
Recombination signal sequence
• To ensure proper rearrangment
RSS :-
• Conserved palindromic sequence
• Conserved Nonamer AT rich
• Intervening sequences :12bp or 23 bp
Antibody diversity
Junctional Flexibility
• Joining of signal sequence: precisely
and coding sequence : imprecise
=
Non productive arrangement : apoptosis
Productive arrangement : Diversity
Antibody diversity
• P addition • N addition:15optional
Recognition of RSS • TdT
Ss break • D..TC ……TA…J….
Hairpin formation D..AG……..AT…J….
Ds break 2) D…TCGA
Generaton sites for ……….ATAT…J
addition of 3)Optional addition of N
Pnucleotides
Antibody Diversity
• Somatic Hyper mutation
– Within somatic cells mutation : new
combination
• Association of Heavy and light genes
combination of heavy and light genes