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What Is Biotechnology?
Using scientific methods with organisms to produce new products or new forms of organisms Any technique that uses living organisms or substances from those organisms to make or modify a product, to improve plants or animals, or to develop microorganisms for specific uses
What Is Biotechnology?
GMO- genetically modified organisms. GEO- genetically enhanced organisms. With both, the natural genetic material of the organism has been altered. Roots in bread making, wine brewing, cheese and yogurt fermentation, and classical plant and animal breeding
What Is Biotechnology?
Manipulation of genes is called genetic engineering or recombinant DNA technology Genetic engineering involves taking one or more genes from a location in one organism and either
Transferring them to another organism Putting them back into the original organism in different combinations
Biotech in 1998
1,300 companies in the US 2/3 have less than 135 employees 140,000 jobs
Jobs will continue to increase exponentially Jobs are available to high school graduates through PhDs
Multidisciplinary- involving a number of disciplines that are coordinated for a desired outcome Science
Ancient biotechnology- early history as related to food and shelter; Includes domestication Classical biotechnology- built on ancient biotechnology; Fermentation promoted food production, and medicine Modern biotechnology- manipulates genetic information in organism; Genetic engineering
Organismic biotechnology- uses intact organisms; Does not alter genetic material Molecular biotechnology- alters genetic makeup to achieve specific goals
Medicine
Thomas Hunt Morgan Discovered how genes are transmitted through chromosomes
Rosalind Elsie Franklin Research led to the discovery of the double helix structure of DNA
Ian Wilmut Created the first true clone, the Dorset ewe Dolly
Molecular biology- study of molecules in cells Metabolism- processes by which organisms use nutrients Anabolism- building tissues from smaller materials Catabolism- breaking down materials into smaller components
What Is a Cell?
Cell- a discrete unit of life Unicellular organismorganism of one cell Multicellular organism- organism of many cells Prokaryote- cells that lack specific nucleus Eukaryote- cells with well-defined nucleus
What Is a Cell?
Tissue- collection of cells with specific functions Organs- collections of tissues with specific functions Organ systems- collections of organs with specific functions
Molecular genetics- study of genes and how they are expressed Chromosome- part of cell nucleus that contains heredity information and promotes protein synthesis Gene- basic unit of heredity on a chromosome DNA- molecule in a chromosome that codes genetic information
Transcription- process of RNA production by DNA DNA-thread-like molecule which decodes DNA information
Kinds of RNA:
mRNA- RNA molecules that carry information that specifies amino acid sequence of a protein molecule during translation rRNA- RNA molecules that form the ribosomal subunits; Mediate the translation of mRNA into proteins tRNA- molecules that decode sequence information in and mRNA snRNA- very short RNA that interconnects with to promote formation of mRNA
Genetic engineering- artificially changing the genetic information in the cells of organisms Transgenic- an organism that has been genetically modified GMO- a genetically modified organism GEO- a genetically enhanced organism
Donor cell- cell that provides DNA Recipient cell- cell that receives DNA Protocol- procedure for a scientific process Three methods used in gene transfer
Agrobacterium gene transfer- plasmid Ballistic gene transfer- gene gun Direct gene transfer- enzymes
5.
6.
Extract DNA from donor Cut DNA into fragments Sort DNA fragments Recombine DNA fragments Transfer plasmids with bonded DNA Grow transformed (recipient) cells
Plant breeding- improvement of plants by breeding selected individuals to achieve desired goals Cultivar- a cultivated crop variety
Line breeding- breeding successive generations of plants among themselves Crossbreeding- breeding plants of different varieties or species Hybridization- breeding individuals from two distinctly different varieties
Selection
Resist pests Resist herbicides Improved product quality Pharmaceuticals Industrial products
Genes inhibiting synthesis of saturated fatty acid successfully inserted in chromosomal DNA of palm oil callus
What Is AI?
Artificial insemination- the transfer of collected semen to a recipient female Semen is collected from males of desired quality Semen is graded and stored
What Is AI?
Female must be in estrus for conception Hormone injections may be used to synchronize estrus Semen is placed in the cervix near the horns of the uterus
In vitro fertilization- fertilization of collected ova outside the reproductive tract; Usually in a test tube
Semen is collected from males of desired quality Ova are removed from females Sperm and ova are placed in a petri dish or test tube
Very young animals receive hormone treatments Most common among selected fish species
Sperm sorted before conception Sperm sorted on basis of chromosome differences X chromosomes produce female offspring Y chromosomes produce male offspring
Embryo transfer- removing fertilized ova (embryos) from donor and implanting in a recipient
Surgical and nonsurgical methods are used to remove and implant A quality donor female can produce more offspring
Hormone injections administered prior to estrus Used with embryo transfer AI may be used to fertilize ova After fertilization, embryos are removed and placed in recipients
OVULATION
OVULATION
Rabit oocyte
SUPER OVULATION
What Is Cloning?
Clone- new organism that has been produced asexually from a single parent Genotype is identical to parent Cells or tissues are cultured
Transgenic animals
Transgenic animals
Transgenic animal
Nuclear transfer
Nuclear transfer
Dolly sheep
Hormone- natural product of glands to produce a response in another part of the body Released by endocrine glands into blood system Hormones are identified, functions determined, and isolated Used to increase growth and production rates bST- promotes milk production in cows pST- promotes lean meat production in swine
What Is Bioremediation?
Bioremediation- using biological processes to solve environmental problems Biodegradation- natural processes of microbes in breaking down hydrocarbon materials Biodegradable- capable of being decomposed by microbes
What Is Phytoremediation?
Plants absorb and break down pollutants Used with heavy metals, pesticides, explosives, and leachate
What Is Composting?
Composting- a process that promotes biological decomposition of organic matter Compost bin- a facility that contains materials for composting In-vessel composting- using enclosed containers for composting
Ethics- knowing right from wrong, and then doing the right idea Bio- living organisms Bioethics- knowing right from wrong with living organisms, and then doing the right idea http://www.oelwein.k12.ia.us/hs/Ag.H ome/biotechethics.htm
Read this article from the school website http://www.oelwein.k12.ia.us/hs/Ag.H ome/dwnld/Ethics.in.Biotech.pdf After you read this article prepare ethical arguments with at least 5 Scientific and Factual claims to support your Premise and Conclusion
Ethical Arguments
Human Cloning Gender Reversal on Livestock Gender Reversal on Humans Genetic Engineering of Plants Genetic Engineering of Humans
Creating a Premise
Human cloning is immoral. Now you must find Scientific and Factual Claims to support your Premise After you have at least 5 Claims, you may conclude, Therefore, human cloning is immoral. You do not have to be for or against any of the topics, its your choice!
Indigenous species