Not to be found in other tissues in trace amounts or under pathological conditions Have a convenient diagnostic time window Reflect as much as possible the evaluation of myocardial damage Some markers are unique to the heart. This occurs in a fairly predictable manner for serum cardiac markers, with a rise, peak, and a fall over time. Each marker tends to have a distinct kinetic pattern in blood
Heart has a high concentration of a particular cardiac marker in relationship to its concentration in other organs or blood. Following myocardial necrosis, dead cardiac myocytes release their cellular contents into blood after tissue reperfusion. Elevated markers have become the definitive diagnostic criteria for AMI
Enzymes Total creatine kinase Creatine Kinase from Heart (CK-MB) Lactate Dehydrogenase Isoenzyme 1:2 ratio (LD1:LD2) Troponins Myoglobin (heme protein) catalyze the reaction of phosphocreatinine to creatinine important for energy utilization. Most circulating CK is from muscle. can be measured by one total enzyme or can be separated into 3 isoenzymes isoenzymes help to detect MI and muscular diseases Isoenzyme CK-I(BB) CK-II (MB) CK-III (MM) produced by smooth muscle, brain tissue heart tissue muscle tissue muscle 0% 2-3% 97-98% brain 97-98% 10-15 % 85-90% Enzymes will disappear from the bloodstream. CK may disappear in less than 36-48 hr after a MI. Total CK Female: 30-135 U/L Male: 55-170 U/L CK increased in the following: Myocardial infarction Progressive muscular dystrophy Alcoholic myopathy Hypothyroidism Exercise Intramuscular injection Specific index of injury of myocardium and muscle Increased Serum CK First enzyme to be elevated after a MI Rise: 6-8 hrs Peak: first 18-24 hrs Duration: 4 days Some patients returns to normal within 16 hrs If CK is elevated then CK-MB taken. CK MB Chronic muscular dystrophies Chronic renal failure Lung disease exacerbation ( acute respiratory exertion) Protocol for Early Detection of AMI Measure CKMB isoforms or Myoglobin Catalyze the reversible oxidation of lactate to pyruvate LD activity is measured by monitoring absorbance at = 340 nm (NADH) Total LD activity has poor specificity Several types of LDH isoenzymes (LDH 1-5) Total LDH, LDH1 and LDH2 are used to detect MI. LDH 1 and LDH 2: hemolytic and macrocytic anemia LDH 4 and LDH 5: liver damage Rises: 12-14 hrs in MI Peak: 36-48 (24) hours Duration: 96-160 hours (10-14 days) Serum LD: 30 IU/L
LD elevated in the following conditions: Carcinomatosis Severe shock Hypoxia myocardial infarction Pulmonary infarction Granulocytic leukemia Infectious mononucleosis Progressive muscular dystrophy Isoenzyme Normal serum Heart muscle Liver Skeletal muscle LDH1 25 45 0 0 LDH2 35 40 5 0 LDH3 20 10 10 10 LDH4 15 5 15 30 LDH5 5 0 70 60 LD1/LD2, Post AMI Increase: 8-12 hrs LD1/LD2 1: 48-72 hrs Return to normal: 8-14 days Troponin is an important component of the contractile machinery of skeletal and cardiac muscle. The troponin complex is located in the thin filaments at intervals set by the length of a tropomyosin unit. Each troponin complex regulates the interactions of 7 actin units. During skeletal and cardiac muscle contraction: the binding of calcium to Troponin C induces a conformational change transmitted to other members of the troponin complex and then to tropomyosin resulting in shortening of the actin-myosin complex and muscle contraction Troponin C (18kd) Calcium-binding subunit No cardiac specificity Troponin I (26.5kd) Actinomyosin-ATP-inhibiting subunit Cardiac-specific form Troponin T (39kd) Anchors troponin complex to the Tropomyosin strand
Recommend as the gold standard, more sensitive than CKMB Troponin I Increase: 4-6 hrs Peak: 20-24 hrs Duration: up to >144 hours Troponin T Increase: 3-4 hrs Peak: 20-24 hours Duration: >240 hours (7-10 to 14 days) Oxygen-binding protein After a MI, myoglobin is the earliest marker of MI but can be elevated by many other factors such as skeletal muscle damage May be used to assess for reperfusion injury of myocardium after thrombolytic therapy Myoglobin = <90 g/L Sensitivity 95-100% poor specificity Serum Myoglobin Levels Increased in: AMI Open heart surgery Exhaustive exercise Skeletal muscle damage Progressive Muscular dystrophy Shock Renal failure Following IM injection Serum Myoglobin Levels Remains Normal: Chest pain without AMI CHF without AMI Cardiac catherization Moderate exercise First marker to appear is myoglobin and peaks at 8 hours after infarction and return to normal within 18-24 hours CKMB 10-12 hours after infarction remains elevated 24 -36 hours, troponin peaks 24 hours after infarction, falls slowly and detectable 7-10 days.