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Plant pathogens:
Nematodes, Fungi, Bacteria, Nematodes, Rikettsia,
Mycoplasma, Viruses and Viroids.
can be transmitted from diseased to healthy
plants.
Pathogen tested Plants
Disease Triangle:
http://urbanext.illinois.edu/containergardening/problems.cfm
Aphids (Insect)
Control:
Insect predators such as lady
beetles, lacewings, syrphid flies
and parasitic wasps.
Fungi that attack aphids.
Plain water from a hose can
wash them off.
Insecticides.
Meristem Culture:
Meristem Culture
Meristem isolation and culture:
Meristem viral free culture ?
VIRUS ELIMINATION
THERAPY:
1. Thermotherapy
Virus elimination procedure:
Preconditioning at High
Temp.
37 to 380C for 2 t0 4
weeks
Heat Treatment
Temp.
30 to 50 0C
Methods:
Water soaking
Expose to hot
air
Expose to
aerated steam
Replication is
significantly Reduced
at elevated
temperature.
Production of viral
movement protein and
coat protein are temp.
sensitive
1.Thermotherapy:
2. Cryo-therapy:
Prolonged exposure of shoot tip culture to a low temperature (
5 0C) .
E.g. In chrysanthemum, shoot tip culture exposed with 50C for
4 months.
Result:
67 % free from chrysanthemum stunt virus (CSV)
22% were free from chrysanthemum chloritic mottle virus (
CCMV).
The frequency is increased to 73 % in case of CSV and to 49
percent in CCMV after 7.5 months exposure to 5 0C.
3. Chemo-therapy:
Incorporation of antiviral drugs into the culture medium
for curing the shoot tips virus.
eg: Virazole, 2-thiouracil, Cyclohexamide.
Virazole: nucleoside analogue
broad spectrum against animal DNA and RNA
viruses
Ribavirin: Guanosine analogue
DHT( %-dihydroazauracil): uracil analoge
shown variable results when applied to the potato
plant as spray or in hydroponiz culture followed by
meristem culture.
SURFACE STERILIZATION :
Ethanol (Alcohol)
Mercuric chloride.
Bactericides and fungicides.
Antibiotics
Gentamycin
Rifampicin
Nystatin + Carbenecillin
Gentamycin + Amphotericin B
Vancomycin-HCI + Mycostatin
Streptomycin + Carbenecillin
http://www.iht.edu.in/mgt-616.html
Growing seedlings:
Reference:
Plant Product Biotechnology By P.R. Yadav
http://pdf.usaid.gov/pdf_docs/PNAAW412.pdf