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Fluorescence
Fiber
By: Team Yellow
September 15,2010
Fiber as
Evidence
Trace evidence
Collected and analyzed when there has been a personal
contact
- homicide, assault, sexual offenses
- hit-and-run, other vehicle accidents
- burglaries
Advantage
- can remain intact for years
- easily transferred
Disadvantage
- class characteristics only
- adhering problems due to type of fiber or type of
receiving
material
Analytical Methods
Polarized light microscopy (PLM)
Microspectrophotometry
The technique of measuring the light absorbed, reflected,
Instrument
Light Source: UV-VIS uses Xenon, Fluorescence
Detector
100 scans of four different yellow fibers where taken and
Emission Spectra
Emission Spectra
Potential problems,
Interferences
When dealing with fibers you must know a head
Conclusion
1. The groups of fluorescence spectra can be
References
Invitrogen. (2010). Product spectra. Retrieved from
http://www.invitrogen.com/site/us/en/home/support/Pr
oduct-Technical-Resources/ProductSpectra.7528p52.html
Microspectrophotometry. The American Heritage
Medical Dictionary. (2007). Retrieved from
http://medicaldictionary.thefreedictionary.com/microspectrophotome
try
Morgan, S. L., Nieuwland, A. A., Mubarak, C. R.,
Hendrix, J. E., Enlow, E. M., & Vasser, B. J. (2004).
Forensic discrimination of dyed textile fibers using
UV-VIS and fluorescence microspectrophotometry.
Proceedings of the European Fibres Group, 25 May
2004. Retrieved from
http://www.chem.sc.edu/faculty/morgan/pubs/Bartick_