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(ROS)
Antioxydant
Virhan Novianry
Asal ROS
1. Respirasi : OH, O, HO2
2. Metabolisme asam lemak : radikal
peroksil (ROO*) radikal alkaloxyl
(RO*)
. ROS : memiliki unsur atom O, selain
itu terdapat radikat lain e.g : carbon
(R), sulphur (RS) or nitrogen (RN)
ROS non-radical
hydrogen peroxide (H2O2)
singlet dioxygen(O2) or
hypochlorous acid (HOCl)
relatively stable but they can be precursors for the generation of more
reactive species
Free radicals could be stabilized in several ways:
If two radicals meet, they can combine their unpaired electrons and
create a covalent bond.
If the free radical reacts with a non-radical molecule two cases may
arise:
i) The free radical may release its unpaired electron (reducing radical).
ii) The free radical may accept the electron from the other molecule
(oxidizing radical).
2nd choice (i et ii) cause chain reaction, non-radical molecule becomea
raddical
PEMBENTUKAN
Dioxygen is bonded inside the HEME-type active site of
cytochrome
Terminasi
two peroxyl radicals meet
two carbon-centred radicals meet
peroxyl radical and carbon-centred
radical meet
DNA Damage
hydroxyl radical (all base)
Singlet oxygen reacts only with
guanine
Malondialdehyde condensation (of
aldehydes) with adenine, guanine or
cytosine
Strategy of antioxidant
defence
Objectives :
to remove oxygen or to decrease local
oxygen concentration
to remove metal ions catalysing ROS
formation
to remove ROS (e.g., O2-, H2O2, OH)
to break the radical chain reaction
to regenerate endogenous antioxidants
to inhibit pro-oxidant enzymes
Prevention
Metal chelation would prevent their formation that , catalyse
by particularly iron and copper ions.
Eg : Proteins (e.g., ferritin and transferrin) or enzymes (e.g.,
gluthathione S-transferases)
Interception
Enzymatic antioxidants
Superoxide dismutase (SOD)
Catalase
Gluthathione peroxidase (GPx)
Non-enzymatic
antioxidants
-tocopherol (vitamin E)
Carotenoids
Gluthatione (GSH)
Reparation
When prevention and interception
fail oxidative products are formed
and accumulate in tissues
i) DNA repair,
ii) degradation of the damaged
proteins or
iii) metabolization of lipid
hydroperoxides formed during lipid
peroxidation
Antioxidant properties of
polyphenolic compounds
inhibit enzymes which are
associated with the production of
ROS (e.g., xanthine oxidase,
cyclooxygenases or lipoxygenases)
1. the presence of the 5OH and 7OH
groups
2. the presence of the 2,3-double bond
3. the absence of the 3OH group
i) Coupled protonelectron
transfer (CPET)
ii) Electron transferproton
transfer (ET-PT)