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SAURABH PANDEY
PALB-3252
SUBMITTED TO:
PROF. DAYAL DOSS
DEPARTMENT OF PLANT
BIOTECHNOLOGY UAS GKVK
BANGALORE
INFORMATION CONTENT
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INTRODUCTION
What is GM crops?
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1980 to 1996
1983 genetically modified tobacco resistant to an antibiotic
1985 first GM crop trials
1993 US Food and Drug Administration allows companies to
market GM seed
1994 Flavr Savr tomato, is approved in the US
1996Herbicide-tolerant GM soya bean available in US
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PUBLIC CONCERNS
Is GM technology safe for plants, animals and humans?
Is GM technology the solution to world hunger?
Is it ethical to alter the genetic make up of a plant?
Does GM technology prove to be a safer, more
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Attitudes to GM foods:
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More than half of the public were not sure whether GM food should be
promoted or not.
For benefit v/s risk:
42% of the sample thought the risks of GM food outweighed the
benefits
23% that they are same
20% that benefits outweigh the risks
The majority ,85% of the public sampled , thought that we do not know
enough about the potential long term effects of GM food on their health.
They were also concerned about the potential negative impact on the
environment of GM crops.
A substantial no. Thought that there could be a range of future benefits,
for the environment, consumers, and developing countries.
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The global hectarage of biotech crops have increased more than 100-fold
from 1.7 million hectares in 1996 to over 175 million hectares in 2013 this
makes biotech crops the fastest adopted crop technology in recent history.
This adoption rate speaks for itself in terms of its resilience and the benefits
it delivers to farmers and consumers.
Millions of risk-averse farmers, both large and small, world-wide,
have determined that the returns from planting biotech crops are
high, hence repeat planting is virtually 100% which is the acid-test
applied by farmers for judging the performance of any technology.
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in the world with 40.3 million hectares (up from 36.6 million in
2012) and is emerging as a strong global leader in biotech
crops.
For the fifth consecutive year, Brazil was the engine of growth
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ANTIBIOTIC-RESISTANCE GENES
In plant transformation antibiotic-resistance genes are used as selectable
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which there is no known mechanism) will not significantly alter the pool
of antibiotic-resistance genes in the environment.
The transfer of intact, functional, antibiotic-resistance genes to gut
flora from ingested plant material is also highly unlikely.
Even if resistance genes were transferred , no real selective advantage
would accrue and the resistant bacterium would be unlikely to survive.
Second, many of the antibiotic-resistance genes commonly found in
GM crops(such as nptII) confer resistance to antibiotics that are not
used to treat disease in humans, their use having been superseded by
less toxic and more-effective alternatives.
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CHARLOCK PLANT
BELONGS TO
Brassicaceae FAMILY
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INSECT-RESISTANCE GENES
The wide range and type of genes used for developing insect resistance
raise questions about their safety for human consumption, given that
certain protease inhibitors and lectins are known to have toxic effects in
mammals.
One of the genes , the snowdrop lectin GNA(Galanthus nivalis agglutinin),
was the first such gene to attract considerable attention following the
suggestion that potatoes carrying transgene might be responsible for
changing the gut lining of rats.
A resolution of the issue is required, since the value of GNA has recently
demonstrated as a means of developing rice resistant to sap-sucking
insects, which are not susceptible to Bt toxins or plant proteases.
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GENE CONTAINMENT
Preventing the transfer of foreign genes from GM crops to other
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3. Terminator technology
Advantages-Seeds are sterile: inducible
Disadvantage-Silencing of transgene results in viable seeds and introgression;
terminator transgenes and desirable trait must remained linked.
eg. In cotton and soybean crops by Delta and Pine Land Co.
4. Apomixis(seeds produced without fertilization)
Advantages-Can be used to fix the heterosis
Disadvantage- Complex trait, not usual in crop plants, a plant may not be 100%
apomictic.
5. Cleistogamy(Fertilization occurs within unopened flowers)
Advantages-Self-pollination prevents outcrossing
Disadvantage- Complex trait, not usual in crop plants, introgression still occurs.
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6.Transgenic mitigation
Advantage-Introduced trait is advantageous or neutral for the
GM crop but deleterious for weeds.
Disadvantage-Does not prevent gene transfer; may cause
extinction of weedy relatives, thus reducing biodiversity.
example; preventing seed-pod shatter , which is deleterious
to weeds,but can actually be an advantage in crops.
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BIG BUSINESS
The threat of terminator technology being introduced led to protests
in many parts of the world, and was seen as yet another example of
big business imposing its wishes on farmers and consumers alike.
Terminator technology has the potential to make GM crops safer by
reducing gene transfer to weedy relatives.
The publicity has, however, focused on its potential use to prevent
farmers from saving a portion of their grain for use as seed in
subsequent years, thus tying them to an expensive source of fresh
seed each year.
Concerns that terminator technology had already been introduced
led to GM cotton crops being burnt in India amid wide-scale unrest.
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FOOD SAFETY
CONCERNS ABOUT GM FOOD SAFETY
The surveys of public feeling highlight the central issue as being the
influence of biotech crops on health, rather than effects on
environment or food security.
Will these so called franken-foods make me ill? Will I be allergic to
them?
This is the natural position to take and one that is really no different
to that which we all have for all types of the food.
The Pusztai affair also affect the public confidence in GM crops. One of
the contributory factors to the public reaction to the Pusztai report
was undoubtedly the loss of public confidence in food safety following
the bovine spongiform encephalopathy(BSE) outbreaks.
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Further issues have hit media that have not necessarily been
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in pollen from event Bt176 and other events containing the same cry1Ab
gene.(1.1 to 7.1 g/g in Bt176, compared with 0.09 g/g in the other two
events).
Because the pollen-specific promoter used to regulate the expression of
one of the cry1Ab constructs in Bt176.
Pollen from Bt176 had an LD50(the median lethal dose) of the order of
100-400 grains/cm when tested on Monarch butterfly larvae, whereas other
two events showed no effect at concentrations of over 1600 grains/cm.
Since Bt176 is no longer available in US maize varieties , the overall conclusion
is that the risk to Monarch butterfly populations from current Bt maize varieties
is low.
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There was considerable confusion about the nature of his initials claims,
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The report itself concluded that feeding transgenic potatoes carrying the
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3.TERMINATOR GENE
TECHNOLOGY/TECHNOLOGY PROTECTION
Involves
three genes
SYSTEM(TPS)
Ribosome inactivating protein (RIP)gene encodes the production of
Ribosome inactivating protein or saporin. which if expressed, does not allow
protein synthesis to take place.
Cre (Recombinase gene) and the
Lox gene
The TPS genes are regulated by the Late Embryogenesis Abundant (LEA)
promoter.
Genes under the control of this promoter are
only transcribed (activated) during late embryogenesis when seeds are
developing.
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TPS was developed to protect the interests of seed companies and, it is claimed, to
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Indian controversies
In India, genetically modified cotton yields
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CONCLUSION
The following is the visionary counsel offered by Norman Borlaug on biotech
crops in 2005
Over the past decade, we have been witnessing the success of plant
biotechnology. This technology is helping farmers throughout the world
produce higher yield while reducing pesticide use and soil erosion. The
benefits and safety of biotechnology has been proven over the past decade
in countries with more than half the worlds population. What we need is
courage by the leaders of those countries where farmers still have no choice
but to use older and less effective methods. The Green Revolution and now
plant biotechnology are helping meet the demand for food production, while
preserving our environment for future generations.
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REFERENCES
International service for acquisition of agri-biotech
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THANK
YOU
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