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Anamika Chatterjee
12BCH002
Guided by:
are
completely
ENZYME KINETICS
MICHAELIS MENTEN EQUATION
v = k3 [ES]
APPLICATIONS
IMMOBILIZATION
Benecial features:
reuse;
suitability
for application within continuous
operations;
their product is enzyme free, therefore further
processing to remove or inactivate the enzyme is not
required;
improved enzyme stability; and
reduced effluent disposal problems.
Advantages :
Enzymes are easily mass manufactured and cheaper
compared to the mining of precious metals which have
an inelastic supply.
Enzymes can also process organic compounds such as
sugars and alcohols, which are exceedingly frequent in
nature.
Most organic compounds cannot be used as fuel with
metal catalysts because the carbon monoxide formed
during the fuel cells working will quickly poison the
precious metals that the cell relies on, rendering it
ineffective.
Sugars and other biofuels can be harvested on a
massive scale, and can be found in nearly any part of
the world, thus making it an amazingly striking option
from a logistics standpoint, and even more so for those
concerned with the adoption ofrenewable energy
CASE STUDY
Two plant based enzymes with diverse
applications were studied and an economic
analysis was performed to evince that enzymes
are cheaper to produce since the advent of
recombinant technologies and hence a more
viable option over industrial catalysts.
BUTYRYL CHOLINESTERASE
It is an enzyme that can act as a bio scavenger to
offset the effects of cholinesterase inhibitors such
as sarin . Also, it is already in use as bio defense
countermeasures in several countries. At present,
BuChE is extracted from outdated human blood
supplies, but it can also be made recombinantly
in cell culture, transgenic animals, and plant
systems
SARIN
CELLULASES
METHODS
SuperPro Designer ,Version 9.0 (Intelligen, Inc.,
Scotch Plains, NJ; http://www. intelligen.com/),
was the software used for the modelling of both
case studies.
It is generally used for techno economic modeling
and is utilized for process simulation and
flowsheet development. It is capable of
performing
mass
and
energy
balances,
equipment
sizing,
batch
scheduling/debottlenecking, capital investment
and operating cost analysis, and protability
analysis.
notably
N.
tabacum,
N.
excelciana,
and
of
various
viral
replicons,
and
high
RESULTS
REFERENCES
[1] Tyler Johannes, Michael R. Simurdiak , Huimin
Zhao, Biocatalysis, Encyclopedia of Chemical
Processing, 2006 , pp 102
[2] http://en.wikipedia.org/wiki/Enzyme, 28/01/15,
20:20
[3] Saha B. et al., Applied Biocatalysis in Specialty
Chemicals and Pharmaceuticals; ACS
Symposium Series; American Chemical Society:
Washington, DC, 2001
[4] Kim Gail Clarke, Bioprocess Engineering,
Woodhead Publishing , 2013