Vous êtes sur la page 1sur 11

REPLICATION , TRANCRIPTION ,AND

TRASLATION OF CHLOROPLAST
Replication begin within two D-loop and
spread out word to form theta structure
Ignition factor, elongation factor
&termination factor function in chloroplast
translation and eubacterial in similar way
Only universal codon formed in chloroplast
DNA
CHLOROPLAST GENOME FOR 4rRNA ,30tRNA & 50 Protein
Gene Type
RNA CODING
16sRNA 1
23sRNA 1
4.5sRNA 1
5sRNA 1
GENE EXPRESSION 30
r-protein 19
RNA polymerase 3
Other 2
THALOID MEMBRANE
Photosystem I
Photosystem II Cytochrome b 2
H+-ATPase 7
Other 3
NADH 6
Ferodoxin
Ribulose B P llase????? 6
Unidentified 3
Total 1
29
110
Reference ---:
B.Levin –Gene viith edition
De robertis –Cell and Molecular Biology
Lubert Stryer –vth edition –Biochemistry
Lehiniger – priciple of biochemistry
Morrae W. Strikberger –Genetics
Benjamin A. Pierce- Genetics
Watson hopkins .robert Stiek.weirner-
Molecular biology of gene
Reference ---:
B.Levin –Gene viith edition
De robertis –Cell and Molecular Biology
Lubert Stryer –vth edition –Biochemistry
Lehiniger – priciple of biochemistry
Morrae W. Strikberger –Genetics
Benjamin A. Pierce- Genetics
Watson hopkins .robert Stiek.weirner-
Molecular biology of gene
11.general two giant precursor RNA that are then
cleaved to yield individual rRNA tRNA &mRNA
12. As tRNA transcribe , they fold up in to three
dimensional configuration (PLAY ROLE
THEEDIMENTIONAL CONFIGURATION
FORMATION)
13. These configuration are recognize and cut by
enzyme
14. So cleavage of tRNA give rise mRNA and
rRNA
15. In mitochondria of fungi and algae &plant
&protist have multiple promoter
Although gene are occasionally arranged and
transcribe in operon
11.general two giant precursor RNA that are then
cleaved to yield individual rRNA tRNA &mRNA
12. As tRNA transcribe , they fold up in to three
dimensional configuration (PLAY ROLE
THEEDIMENTIONAL CONFIGURATION
FORMATION)
13. These configuration are recognize and cut by
enzyme
14. So cleavage of tRNA give rise mRNA and
rRNA
15. In mitochondria of fungi and algae &plant
&protist have multiple promoter
Although gene are occasionally arranged and
transcribe in operon
REPLICATION TRANSCRIPTION,
TRANSLATION OF MITOCHONDRIAL DNA
1.Mmitochondrial DNA does not replicate in the
orderly regulated manner as of nuclear DNA
2.mitochondrial DNA synthesized throughout cell
cycle
3.and Is not coordinated with the synthesis of
nuclear DNA
edalgarno sequence No Yes Rare Some
nslated region of

versal codon Rare Rare Yes No


tion inhibited by No Yes Yes Yes
line
d bubble No No Yes No
COMPARISION OF NUCLEAR,
EUKARYOTIC, EUBACTERIAL,
MITOCHONDRIAL&CHLOROPLAST

aracteristic Euk Eub Mito Chlo


me consist of dss – yes yes yes yes

ar No Yes Most Yes


e protein Yes No No No
Large Small Small Smal
l
molecule genome No Yes Yes in Yes
animal
no in
plant
RNA intron Common Absent Absent Abse
nt
I intron Present Present Present Prese
nt
II intron Absent Present Present Prese
nt
tronic mRNA Uncomm Common Present Com
on mon
dded to mRNA Yes No No No
A tail added to Yes No Some in No
animal
C CODE IS ALTER IN MITOCHONDRIA
m Stop IIe Arg Leu ARG
UGA AUG AGG CUN CCG
AGA
L

te Trp Meth Stop + +


la Trp Meth Ser + +

is globrata Trp Meth + Thr ?


yceae cerviceae Trp Meth + Thr +
chromyces Trp + + + +

us fungi Trp + + + +

some Trp + + + +
lant + + + + Trp
ydomonas ? + + + ?
lii

?= indicate that codon has not been observed in the

Vous aimerez peut-être aussi