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ANALYSIS
DEPARTMENT OF CLINICAL PATHOLOGY
MEDICAL SCHOOL- UNIVERSITAS PADJADJARAN/
RUMAH SAKIT DR. HASAN SADIKIN
BANDUNG
INCREASED AMOUNT :
acute and chronic congestion of
the meningens due to increased
transudation; acute and chronic
infections
Sample collection of
CSF:
(PRE-ANALYTICAL STEPS)
1.
DIAGNOSTIC or
2.
THERAPEUTIC
DIAGNOSTIC :
1.
2.
THERAPEUTIC:
1.
2.
CONTRAINDICATIONS :
1.
Subtentorial tumors
2.
(PRE-ANALYTICAL STEPS).
1.
2.
3.
microbiological examination
cell count and differential count
Macroscopic examination:
Color,
Normal: clear
and colorless
ROUTINE
ANALYSIS:
(ANALYTICAL
STEPS)
Turbidity,
Normal: clear
Macroscopic examination:..
Microscopic
examination:
CHEMICAL EXAMINATION :
centrifuged
NONNE-APELT TEST.
GLOBULIN
NONNE-APELT TEST..
NONNE-APELT TEST.
NONNE-APELT TEST
ALBUMIN
Shake the tube used in globulin test
Add 1 drop of 10% acetic acid, boil
Report as follows:
Negative = a slight cloudiness
+ = a definite cloudiness, with a
fine precipitate
NONNE-APELT TEST
++ = a flocculate precipitate in a
slightly
cloudy fluid
+++ = a heavy flocculate precipitate
in a
clear fluid
PANDYS TEST
1cc phenol (saturated aqueous
solution) + 1 large drop of CSF
Normal CSF may show a faint trace,
and should be report as NEGATIVE
POSITIVE :
a bluish-white cloud forming
immediately around the drop of CSF
:abnormal amount of globulin