The fabrication and characterization of

poly (dimethyl-co-methylmethacrylate)
siloxane/ poly (ethylene glycol)
diacrylate for tissue engineering (TE)
application

Name: Lim Kar Wai

Lecture: Dr. Zuratul Ain Abdul Binti Hamid
Date: 9 October 2015

Tissue engineering
application
Biomaterial
scaffold
Growth
factor/biorea
ctor

Cells

Enginee
red
tissue

Polymeric scaffolds

Tissue repair

Evolution of scaffold materials in engineered tissue

1980
1960

1940

2000
until
now

Criteria for scaffolding materials in tissue

engineering applications
Biocompatibi
lity

Mass
transport
properties

Tissue
engineering

Mechanical
integrity

Biodegradabi
lity

Materials studied

hydrophobic inorganic
polymers
(matrix)
biocompatibility
excellent gas
permeability
highly flexible when
lightly crosslinked
What is the current
limitation in TE
application?

hydrophilic organic
polymers
(crosslinker)
biocompatibility
excellent water
permeability
Study to match the
compromise
properties for TE
application and
looks for commercial
values

Improvement

Flow chart of the research study

Tissue
engineering
application

Sample
preparation and
characterization

Material
development
Literature
review

Research objective:
To fabricate a 3-dimensional porous structure hydrogel
with excellent mass transport properties

Step 1: Synthesis of poly (dimethyl-comethylhydrido) siloxane precursor solution

(PDDMHS)

Chemical formulation for synthesis of PDMMHS

Molecular
weight= 6000

Percentage of Si-H group along the molecular chain= 15 %

Material

Molecular weight,

Octamethylcyclosiloxane
296.62
Octamethylcyclosiloxane (D4)
(D4)
296.62
1,1,4,4240.51
1,1,4,4240.51
tetramethylcyclosiloxane
tetramethylcyclosiloxane
(D4H)
(D4H)
Hexamethyldisiloxane
162.38
Hexamethyldisiloxane
162.38
(HMDS)
(HMDS) 162.38+ m(296.62)+m(15/100)(240.51)=6000

m~18 and n~3

Hence, 1 mole of HMDS react with 18 mole of D4 and 3 mole of D4H to
produce PDMMHS solution with molecular weight of 6000 with 15 % Si-H
moiety.

Chemical

Concentration
(mmole)

Mass
(g)

Boiling point
( C)

Volume
(L)

D4

67.43

20.0

175-176

D4H

11.24

2.7

3.75

0.6

162

20

HMDS
Trifflic acid

Let mass of D4= 20g

Concentration= 20/296.62= 0.0674 mole
Hence, mole of D4H needed=0.0674 3/18= 0.01124 mole
mass od D4H needed= 2.70g
Hence, mole of HMDS needed= 0.0674 1/18= 0.00375 mole
mass of HMDS needed= 0.00375 162.38= 0.60 g

Step 2: Synthesis of poly (dimethyl-comethylmethacrylate) siloxane precursor solution

(PDMMMS)

Step 3: Hydrogel fabrication

UV-crosslinking using UV chamber equipped with Philip
uv-A tube that emit energy in the wavelength region of
300-400 nm.
Photoinitiator used: Irgacure 651
Parameter studied: Different weight ratio of PDMMMS and
PEGDA
Characterization:
Chemical FTIR, gel content, swelling ratio, thermal
properties, mechanical properties and morphology

Thank You