Microscope Review

Give the name and function

of each structure
labeled.
A Ocular: eyepiece used to
look at specimen
B Fine adjustment: to focus
specimen under high power
C Arm: used to hold
microscope
D Objective lens: used to
magnify image
E Coarse adjustment: Used to
focus specimen under low
power
F Diaphragm adjust amount
of light reaching specimen

What was the highest

possible
magnification that
can be obtained
when using this
microscope?
Objective - Ocular
40 x 10 =
400x

What happens to the

amount of light in the
field of view when
switching from low to
high power?

The amount of light

decreases (so the
field of view gets
darker)
F

What happens to the

field of view when
switching from low to
high power?

The field of view

decreases ( so you
see less of the
slide).
F

Which structure can only

be used to focus the
specimen under high
power? Support your
answer.
The fine adjustment
because using the
coarse adjustment
under high power can
break the objective
lens or the slide.

What are two possible adjustments that

need to be made when switching to
high power?
Adjust the diaphragm to make the filed
of view brighter.
Focus with the fine adjustment.

What happens to the number of cells

that are visible in the field of view
when switching to high power?
The number of cells that are visible in
the field of view decreases.

What must be done to the

microscope before switching from
low power to high power?
Center the specimen
Focus with the coarse adjustment

Which laboratory technique is

illustrated in the diagram?
Preparing a wet mount.

Describe the steps to follow when

preparing a wet mount.
1. Place a drop of water on slide.
2. Place specimen on slide.
3. Place the cover slip at an angle.
4. Slowly lower the cover slip on to the
specimen.

Why must the cover slip be placed

at an angle when preparing a wet
mount?
To avoid air bubbles.

The diagram represents a cell in the field of

view of a compound light microscope. In
which direction should the slide be moved
on the microscope stage to center the cell
in the field of view?

Towards C

The image to the left is placed on the

slide. A student then views the
letter f under low power. Which
diagram below most closely
resembles the image under low
power?

A student sees the image to the left

when observing the letter "f" with
the low-power objective lens of a
microscope. Which diagram below
most closely resembles the image
the student will see after
switching to high power?

The total magnification of an image is

the result of the combined
magnifications of the
1) eyepiece and diaphragm
2) objective and eyepiece
3) objective and mirror
4) low-power and high-power
objectives

A student views some cheek cells

under low power. Before switching
to high power, the student should
(1) adjust the eyepiece
(2) center the image being viewed
(3) remove the slide from the stage
(4) remove the coverslip

A student changes the objective of a

microscope from 10x to 50x. If
this is the only change made, what
will happen to the field of view?
(1) Its diameter will decrease.
(2) Its diameter will increase.
(3) Its brightness will increase.
(4) Its brightness will remain the
same.

A student determined that exactly 10

pollen grains could fit along the
diameter of the field of view of a
microscope. If each pollen grain has a
diameter of 200 micrometers, what is
the diameter in millimeters of this
microscope's field of view?
(1) 2,000 mm
(2) 2 mm
(3) 5 mm
(4) 500 mm

A student is using a compound light

microscope to observe a wet mount of
unstained human cheek cells. Which
cell organelle will most likely become
more visible after she adds iodine
solution to the slide?
(1) nucleus
(2) ribosome
(3) cell wall
(4) mitochondrion

To locate a specimen on a prepared slide with a

compound microscope, a student should begin with
the low-power objective rather than the highpower objective because the
(1) field of vision is smaller under low power than
under high power
(2) field of vision is larger under low power than
under high power
(3) specimen does not need to be stained for
observation under low power but must be stained
for observation under high power
(4) amount of the specimen that can be observed
under low power is less than the amount that can be
observed under high power

An unstained wet mount preparation

of cheek cells shows few details.
Which substance could be added
to the slides to make the details
more visible?
(1) methylene blue
(2) dilute acid
(3) Benedict's solution
(4) bromthymol blue

After switching from the high-power to

the low-power objective lens of a
compound light microscope, the area
of the low-power field will appear
(1) larger and brighter
(2) smaller and brighter
(3) larger and darker
(4) smaller and darker

The diagram represents a hydra as viewed with

a compound light microscope. If the hydra
moves to the right of the slide preparation,
which diagram below best represents what
will be viewed through the microscope?

A cover slip should be used for

preparing a
(1) frog for dissection
(2) solution of iodine for food testing
(3) wet mount of elodea (a simple plant)
(4) test to determine the pH of a
solution

Which part of a microscope should be

used with the low power objective,
but not with the high-power
objective?
a. fine adjustment
b. diaphragm
c. coarse adjustment
d. eyepiece

A prepared slide was placed on the stage of a

microscope so that the slide faced the
student, as shown in the diagram below.

Which image will the student most likely see?

Convert:
1. 6.23mm
2. 5678um
3. .093mm
4. 48.2um

6230
= ______
um
5.678
= ______
mm
93
= ______
um
.0482
= ______
mm

The diagram represents the field of view of a

compound light microscope. Three
unicellular organisms are located across the
diameter of the field. What is the
approximate length of each unicellular
organism?
1) 250 um

2) 500 um 3) 1,000 um 4) 1,500 um

This organism could be centered in the field

of view by moving the microscope slide
towards
(1) A
(2) B
(3) C
(4) D

250um

500um

125um

The diameter is 1mm. What is the length

of the cell in um? 375um

1750um

3.5mm
3500um

The length of the cell is approximately 1750um.

There are 3 cells

across the field
of view.
1500/3 = 500um

Field of view = 1.5mm

= 1500um