Académique Documents
Professionnel Documents
Culture Documents
NRL-East
Avenue Med
Center
(Toxicology)
NRL-RITM
(Microbiology
/ Testing for
donors)
NRL-NKTI
(Hematolo
gy)
NRL-SACCL
San Lazaro
Hospital
(HIV/AIDS,
Hepatitis,
and other
STIs)
NRL-Lung
Center of
the
Philippine
s
NRL-SACCL
NRL-SACCL (National
Reference Laboratory
STD AIDS
Cooperative Central
Laboratory), San
Lazaro Hospital,
Manila
- NRL for HIV and other
STIs
3. To maintain a
quality assurance
program for
laboratory tests- in
coordination with
BHFS-DOH
2. To train laboratory
personnel
Referral System
SEE
GROW
REACT with chemicals/reagents
Antibody substance
produced by the body
against the antigen
Level
window period
Seropositive
Antigen
Threshold
for detection
Seronegative
w e e k s
m o n t h s
Time frame
Screening tests
-Initial test/s done
-Must be sensitive
-HIV proficient RMTs
-If reactive, proceed
to confirmatory
Confirmatory tests
-A more specific test
-No biological false +
-Trained personnel
-Results must be
correlated to
screening tests and
clinical findings
Monitoring tests
-Test/s done after a
POSITIVE
confirmation
-To monitor disease
progression and
treatment response
- ARVs
- CD4 / Viral load
- Nucleic acid tests
- DNA sequencing
RBC
Mono
Lym
Neu
Eos
Baso
HIV tests
Screening:
Particle agglutination
ELISA
Immunochromatography
Confirmatory:
Western Blot
Immunofluorescence
Assay
Monitoring:
CD4 (flowcytometry)
Viral Load (NAT)
Hepa C tests
Screening:
EIA, Rapid tests
Hepa B
tests :
Screening
ELISA /
Immunochromatography
Confirmatory:
Neutralization Assay
Monitoring
NAT
Confirmatory:
SIA (e.g. RIBA,LIA), HCV
RNA(Qualitative)
Confirmatory:
TP Particle Agglutination (TP-PA)
TP Heme Agglutination (TP-HA)
Fluorescent Treponemal Antibody
Absorption (FTA-Abs)
Monitoring
HCV RNA (Quantitative)
Monitoring:
RPR Quantitative
Standardized Reporting
Serology (HIV, Hepatitis, and Syphilis)
Screening tests
REACTIVE
NONREACTIVE
Inconclusive
Confirmatory tests
POSITIVE
NEGATIVE
Indeterminate
medical
technologist
patient
Laboratory
(screening)
doctor
result
DOH-NEC
(AIDS Registry)
NRL-SACCL
(confirmatory)
DOH team
NRL-SACCL
(Screening &
Confirmatory)
Social Hygiene
Clinics (SHC)
DOH-NEC
result
STEP 2 Transfer
NEW!
STEP 3 Pack
Absorbent material
Ice gel / cold dog
Primary container
Styrobox
Secondary container
STEP 4 Send
3. To maintain a
quality assurance
program for
laboratory tests- in
coordination with
BHFS-DOH
2. To train laboratory
personnel
SECONDARY:
1. to identify common errors and recommend
corrective measures;
2. to encourage good laboratory practice, using
standardized procedures, appropriate
definitions and high-quality reagents;
3. to encourage the implementation of quality
assurance and control measures in the
participating laboratories;
SECONDARY:
4. to stimulate information exchange and
networking among laboratories at the national
and/or international level;
5. to provide updated information on new
developments in HIV diagnostics and related
matters.
EQA Methods
Proficiency
Proficiency
Testing
Testing
On-site
On-site
Evaluation
Evaluation
Re-checking/
Re-checking/
Re-testing
Re-testing
EQA Methods
Proficiency
Proficiency
Testing
Testing
On-site
On-site
Evaluation
Evaluation
Re-checking/
Re-checking/
Re-testing
Re-testing
EQAS Methods
Proficiency
Proficiency
Testing
Testing
On-site
On-site
Evaluation
Evaluation
Re-checking/
Re-checking/
Re-testing
Re-testing
EQAS Methods
Proficiency
Proficiency
Testing
Testing
On-site
On-site
Evaluation
Evaluation
Re-checking/
Re-checking/
Re-testing
Re-testing
EQA
EQA
Identify
Identify
Problems
Problems
Take
Take
Corrective
Corrective
Action
Action
Corrective Action
An action taken to correct a problem or deficiency
Examples:
Production of an incorrect result
Not following procedures
NEQAS FLOWCHART
NRL sends invitation
for participation
Participant submits
registration form
with payment
Analysis of results
NRL provides results to participants
NRL issues certificate of participation
to participants
NRL provides BHFS list of participating
laboratories
www.doh.gov.ph/saccl
nrl_saccl@yahoo.com