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DETERMINATION
OF THE STOICHIOMETRY OF A
COMPLEX
Lay, Henry
Sison, Kris Jester Franco IV
Group 7
Objectives
Determine
the formula
of a colored complex by
continuous
variation
method
mole ratio method
slope ratio method
TERMINOLOGIES
Spectroscopy-
Spectrometry-
Spectrophotometry-
spectrometry of the
absorption of UV, visible, or infrared radiation
TERMINOLOGIES
SPECTROPHOTOMETRY
IMPORTANCE OF
SPECTROPHOTOMETRY
Applied
LIGHT
Waves
Photons
In
Colored
Spectrophotometer
PERCENT TRANSMITTANCE
AND ABSORBANCE
PERCENT TRANSMITTANCE
AND ABSORBANCE
Beers Law : A = bc
For
Methodology
Preparation of Reagents
Methodology
Continuous Variation Method
6 50mL volumetric flasks labeled A-F
Methodology
Continuous Variation Method
flask
Fe(mL)
10
Phen(m
L)
10
Methodology
Mole ratio Method
6 50mL volumetric flasks labeled A-F
Methodology
Mole ratio Method
Flask
Phen
(mL)
12
15
Methodology
Slope ratio Method
5 50mL volumetric flasks labeled A-E
Phen
(mL)
Methodology
Methodology
0.7
M hydroxylamine
hydrochloride (NH2)OH. HCl
4.8643g in 100mL
HOAc/NaOAc
buffer at pH=4
Requires
Results
Flask
Continuous
Volume of solution
(mL)
Iron (II)
Phenanthrol
ine
variations method
Mole Fraction
Iron (II)
Phenanthrol
ine
Absorban
ce
10
1.0
0.012
0.2
0.8
0.402
0.4
0.6
0.304
0.6
0.4
0.159
0.8
0.2
0.100
10
1.0
0.016
Results
Continuous
variations method
Results
Xfe2+ = 0.25 = 1
Xc12h8n2
To
0.75
Results
Flask
A: iron(II) = 0mL/10mL = 0
Flask
B:
Mol Fe(II)=
(0.0007M Fe)(2mL x 1L/1000mL)= 1.4x10-6
Mol phen=
(0.0007 M phen)(8mL x 1L/100mL)= 5.6 x10-6
mole fraction for Fe=
molFe
1.4 x10
0.2
6
6
totalmol (5.6 x10 ) (1.4 x10 )
of the spectrophotometric
techniques that have been used for the
study of complexformation equilibria
Results
Flask
Mole-ratio
Number of moles
Method
Phenanthroli
ne
Iron (II)
moles of
phenanthroline
moles of Iron (II)
Absorban
ce
7x10-7
1.4x10-6
0.5
0.047
2.1x10-6
1.4x10-6
1.5
0.152
3.5x10-6
1.4x10-6
2.5
0.244
5.6x10-6
1.4x10-6
4.0
0.257
8.4x10-6
1.4x10-6
6.0
0.236
1.05x10-5
1.4x10-6
7.5
0.253
Results
Mole-ratio Method
Mole-Ratio Method
0.3
0.25
0.2
0.15
Absorbance
0.1
0.05
0
0.5
1.5
2.5
7.5
Results
Mole-ratio Method
x=3,
It
Flask
A:
B:
C:
Flask D:
Flask E:
Flask F:
Results : Slope-ratio
method
can
based
Results
Slope-ratio
Flask
mL of C12H8N2
[C12H8N2] in M
Absorbance
1.4 x 10-5 2.8 x 10-5 4.2 x 10-5 5.6 x 10-5 7.0 x10-5
0.050
0.099
0.147
0.195
0.253
Results
0.3
0.25
0.2
0.15
0.1
0.05
Absorbance
[C12H8N2]
Results
Flask
mL of Fe (II)
0.5
1.0
1.5
2.0
2.5
[Fe (II)] in M
7.0 x 10-6
1.4 x 10-5
2.1 x 10-5
2.8 x 10-5
3.5 x 10-5
Absorbance
0.053
0.118
0.197
0.259
0.318
Result
s
0.35
0.3
0.25
0.2
0.15
0.1
0.05
0
Absorbance
[Fe2+]
Slope-ratio method
Results
Constant Fe:
Constant phen:
Results
Slope-ratio method
m fe
m ph
3585.714286
0.374
9585.714286
Discussion
Discussion
N
Fe2+ +
Fe2+
3
N
1,10-phenanthroline
3
Discussion
Discussion
phen)3Fe+2
(phen)3Fe+3 + e-1
red-orange
2Fe+3
pale blue
Discussion
Discussion
Conclusion
THE END
PICTURES!!!