THE EFFECT OF CATERPILLAR FUNGUS

(Cordyceps sinensis [Berk.] Sacc.)

TOWARDS IL-2 LEVEL IN
PARACETAMOL-INDUCED MICE
(Mus musculus L.)
YOHANES IRSANDY
0510105

FACULTY OF MEDICINE
MARANATHA CHRISTIAN UNIVERSITY
BANDUNG
2009
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BACKGROUND
Paracetamol Analgesic, Antipiretic, and
Antiinflammation
Side effect hepatotoxicity
Long term usage of paracetamol may
cause liver damage

BACKGROUND cont
Paracetamol
Cytochrom P450
(Van de straat et al.,
1987)

Biotransformation

Lipid Peroxidation
reaction
(Manson, 1992)

Free radicals

N-acetyl-pbenzoquinone
imine (NAPQI)
LIVER DAMAGE

Very reactive &

Toxic substance
Th1 cells

IL-1 IL-2 IL-6 IFN-TNF-

Th2 cells
IL-4 IL-5 IL-6 IL-10 IL-13

BACKGROUND cont
Cordyceps sinensis is one of a traditional
herbal medicine, which has been widely used
by the ancient chinese
Cordyceps sinensis (917,92 mg/kg BW)
could decrease serum AST & ALT level in
paracetamol-induced mice (Emily, 2008)
Cordyceps sinensis used as alternative therapy
for many kind of diseases (Manson, 1992)
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PROBLEMS
Does Cordyceps sinensis decrease
serum IL-2 level in paracetamol-induced
mice

OBJECTIVE
Examine the effect of Cordyceps
sinensis against serum IL-2 level in
paracetamol-induced mice

RESEARCH BENEFICIALS
Academic beneficial: to expand the
knowledge about herbal medicine,
particularly Cordyceps sinensis
Practical beneficial: to explore
scientific application for Cordyceps
sinensis as hepatoprotector

FRAME OF WORKS
Paracetamol

Liver Damage

serum IL-2 level

Cordyceps sinensis

serum IL-2 level

HYPOTHESIS
Cordyceps sinensis decrease serum IL-2
level in paracetamol-induced mice

EXPERIMENTAL DESIGN
Comparative prospective laboratory
experiment with complete randomized design
Measurement of serum IL-2 level ELISA
method
Data One-Way ANOVA & Tukey HSD

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TIME AND PLACE

Time : June 2008 January 2009
Place :
Pusat Penelitian Ilmu Kedokteran (PPIK),
Faculty of Medicine-Maranatha Christian
University, Bandung

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MACROSCOPIC OF LIVER

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MICROSCOPIC OF LIVER
Sinusoid
Spatium
Disse

Central
vein

Liver cells
Kupffer cell
Billiaris canaliculi

Terminal
limfatic
Limfatic vein
Portal vein
Hepatic artery
Billiaris Duct
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LIVER FUNCTION
1. Metabolic function
2. Haematologic function
3. Bile secretion

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LIVER PATHOLOGY
1.
2.
3.
4.
5.
6.

Degeneration and accumulation intracellular

Necrosis and apoptosis
Inflammation
Regeneration
Fibrosis
Cirrhosis

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PARACETAMOL (Acethaminophen)

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INTERLEUKIN 2 (IL-2)
blastogenic factor, T cell growth factor,
lymphocyte proliferating factor

Function:

T cell growth factor

Stimulate NK cell & Limfosit B proliferation
NK cell differentiation
increase NK cell killing effect

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BIOLOGIC ACTIVITY

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CATERPILLAR FUNGUS
(Cordyceps sinensis [Berk.] Sacc.)

Divisio
Subdivisio
Class
Ordo
Famili
Genus
Species

: Fungi
: Ascomycota
: Sordariomycetes
: Hypocreales
: Clavicipitaceae
: Cordyceps
: Cordyceps sinensis (Berk.) Sacc.
(Holiday and Cleaver, 2004)
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ACTIVE COMPOUNDS
-

cordycepin (3deoxyadenosin)
cordycepic acid (d-mannitol)
23 dideoxyadenosine
complex polysaccharides
(Holiday and Cleaver, 2004)

- Essential Amino Acid

- Vitamins (B1, B2, B12, E, and K)
(Mizuno, 1999)

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Cordycepin (3deoxyadenosin)
# Function:
- Reduce mRNA procollagen I and II
- Inhibits deposit of collagen
- decrease TIMP-2 and TIMP-3
(Kenyon, 2005)

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MATERIAL & METHODS

DDY mice, 20-25 gram, 8 weeks old
All mice were adapted for 7 days

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Treatments
:
Blood

Blood
sample

sample
n=6

d1

d5 d6

Group:
1. CMC 1% (0,4mL/day)
2. Paracetamol (400 mg/kg BW/day)
3. CMC 1% (0,4mL/day)
4. Paracetamol (400 mg/kg BW/day)
Group 1
Group 2
Group 3
Group 4

d12

Group:
1. CMC 1% (0,4mL/day)
2. CMC 1% (0,4mL/day)
3. CS (917,92 mg/kg BW/day)
4. CS (917,92 mg/kg BW/day)

Negative Control
Paracetamol
CS
Paracatamol + Cs

All treatments were administered orally

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24

Average of Serum IL-2 Level among Group

Treatments
Groups

Serum IL-2
Level
Day 5 Day 12

Negative Control

1,296

Paracetamol

19,515

40,979

Cordyceps sinensis

0,986

3,819

Paracetamol +
Cordyceps sinensis

22,367

3,681
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Paracetamol increased serum IL-2 level at Day 5

IL-2 Level (pg/mL)

80
** P = 0,007
60

** P = 0,010

40

20

0
Control

Paracetamol

CS

Paracetamol +
CS

D-5
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Cordyceps sinensis decrease serum IL-2 level at Day 12

IL-2 Level (pg/mL)

80

** P = 0,000

** P = 0,000

60

** P = 0,000

40

20

0
Kontrol

Parasetamol

CS

Parasetamol +
CS

D-12
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DISCUSSION
In paracetamol-induced mice IL-2
level increased significantly
Cordyceps sinensis (917 mg/KgBW)
reduce significantly IL-2 Level in
Paracetamol-induced mice

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DISCUSSION cont
Paracetamol

Inflammation
NK cells

CS
TNF-

IL-2

LIVER DAMAGE

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Conclusion
Cordyceps sinensis has an ability to
decrease serum IL-2 level in
paracetamol-induced mice

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THANK YOU

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