Reunion Plenaria Red de Agavaceas

Proyecto BEI-AGA-11-7
Evaluacin de variedades de Agave de
Tamaulipas con fines de registro.

IPN/Centro de Biotecnologa Genmica

Angel Salazar Bravo
Cuauhtmoc Jacques-Hernndez
Irapuato, Gto. Sep 27, 2012

(A) Male Aedes albopictus, 24 h after death,

showing hyphal growth of the entomopathogenic
fungus Metarhizium anisopliae

B) A cadaver of a male Ae. aegypti, 72 h after death,

showing sporulation of M. anisopliae.
Scholte EJ, Takken W, Knols B (2007) Infection of adult Aedes aegypti and Ae. albopictus
mosquitoes with the entomopathogenic fungus Metarhizium anisopliae. Acta Trop 102: 151158.
pmid:17544354 doi: 10.1016/j.actatropica.2007.04.011

FIGS. 120. Mature conidiogenous cells

and conidia of Metarhizium species.

1. Phialides with developing conidia.

2. Mature conidia.
34. M. guizhouense. 3. Phialides with developing
conidia
(ARSEF 7507). 4. Mature conidia
(ARSEF 6238).
56. M. pingshaense. 5. Phialides with developing
conidia
(ARSEF 4342). 6. Mature conidia
(ARSEF CBS 257.90).
78. M. anisopliae. 7. Phialides with developing
conidia
(ARSEF 7487). 8. Mature conidia
(ARSEF 7487).
910. M. robertsii. 9. Phialides with developing
conidia
(ARSEF 727). 10. Mature conidia
(ARSEF 727).
1112. M. brunneum. 11. Phialides with developing
conidia (ARSEF 4179). 12. Mature
conidia (ARSEF
2107).
1314. M. lepidiotae. 13. Phialides with developing
conidia (ARSEF 4628). 14. Mature
conidia
(ARSEF 7412).
1516. M. acridum. 15. Phialides (ARSEF 6592);
note that
apices of conidiogenous cells
thicken
with
successive conidial
production. 16. Mature conidia (ARSEF 5736).
1718. M. globosum. 17. Phialides (ARSEF 2596).
18.
Mature conidia (ARSEF 2596).
19. M. flavoviride. Mature conidia (ARSEF 2025).
20. M. frigidum. Mature conidia (ARSEF 4124).

Bischoff, F, Joseph; Stephen, A, Rehner and Richard, A, Humber. 2009. A multilocus phylogeny
of the Metarhizium anisopliae lineage. Mycologia, 101(4) 512-530.

(1a)

(1b)

(2a)

(2b)

Fig. 1. Colony features of Metarhizium anisopliae MET

08/I05 and M. pingshaense MET 13/I68 on PDA and
SDAY. The MET 08/I05 colony is dark green while MET
13/I68 is lighter grayish-green on both PDA and SDAY.
On PDA, MET 08/I05 produces some aerial mycelium
(1a) not present in MET 13/I68 (1b), which has a diffuse
conidia production denser at the center of the colony
and organized in a dense pattern of concentric circles.
The MET 13/I68 colony shows the same circular
arrangement on SDAY (2b) while MET 08/I05 exhibits a
rosette-like pattern of growth, an irregular thick crust of
conidia in the center, a whitish, almost sterile zone of
mycelium and a light marginal strip of sporulation (2a)

A
Fig. 2. Conidia and phialides of (a) Metarhizium
anisopliae MET 08/I05 and (b) M. pingshaense MET
13/I68 observed by SEM.

Cito A., Mazza G., Strangi A., Benvenuti C., Barzanti G.P., Dreassi E., Turchetti T., Francardi V.,
Roversi P.F., 2014 Characterization and comparison of Metarhizium strains isolated from
Rhynchophorus ferrugineus. FEMS Microbiol Lett., 355: 108-115.

Fig. 1: Cultural morphology of 14 day old M. anisopliae var.

anisopliae LR2 isolate (a) LR2 PDA, (b) MG PDA, (c) TA
PDA (d) LR2 PDAY (e) MG PDAY (f) TA PDAY (g) LR2 SDA
(h) MG SDA (i) TA SDA (j) LR2 SDAY (k) MG SDAY (l) TA
SDA
Potato Dextrose Agar (PDA), Potato Dextrose Agar with 1% yeast extract (PDAY), Sabaroud
Dextrose Agar (SDA) and Sabaroud Dextrose Agar with 1% yeast extract (SDAY) was assessed

Hoe, P., C. Bong, K. Jugah, and A. Rajan. 2009. Evaluation of Metarhizium anisopliae var.
Anisopliae (Deuteromycotina: Hyphomycete) isolates and their effects in subterranean termite
Coptotermes curviginathus (Isoptera:Rhinotermitidae). American Journal of Agricultural and
Biological Sciences., 4(4):289-297

Seplveda Sanhueza, Mara Esperanza (2015) Actividad

enzimtica e insecticida de seis cepas nativas de
Metarhizium spp. para el control de Aegorhinus
superciliosus (Coleoptera: Curculionidae). Tesis
Universidad de Concepcin
http://repositorio.udec.cl/handle/11594/1686?show=full