Académique Documents
Professionnel Documents
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Principle
1)the cloned gene is ligated into the vector in reverse
orientation
2)
Applications
Flavr Savr
Calgene in 1992
Licensed in may 17 May 1994
ripening of an tomato causes production of an enzyme
polygalatourodase in a gradual increasing level
Responsible for
softening of the tomato
a cause of rottening so the tomato never lasts for few extra days
in ripening condition without rottening
Calgene introduced a gene in plant which synthesise a
complementary mRNA to polygalactourodase and inhibit the
synthesis of PG gene
Indian contribution
NIPGER( National Institute of Plant Genome Research )
Injecting Antisense RNA that is complementary to the protooncogene BCL-2 may be useful for treating some B-cell lymphomas
and leukemias
used for treating brain cancer malignant glioma and cancer of
prostate gland,
Research paper
The sense of 'antisense' RNA: Noncoding antisense RNA can be used to
stimulate protein production
Applications