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Ag/AgCl
Inner solution
analyte
Inner solution
Frit
Evaporated Ag film
Insulator layer
aH +
KaCO 2
a HCO 3
1.CO 2 + H2 O H 2 CO 3
H + + HCO 3 2H + + CO 2 2
2.NH3 + H 2 O NH + 4 + OH
3.H 2S + H2 O HS + H 3 O +
(1)
AstheindicatorisonlyH+sensitive,andthepotentialofthereferenceisa
constant(becauseoftheconstantchlorideconcentrationintheelectrolyte),wehave
Ecell K1 0.059log aH
(at25o C)
(2)
CO2 penetratesthroughthegaspermeablemembraneandwillreactwiththe
electrolyteintheagarhydrogel:
CO2 +H2 O=H++HCO3
(3)
(4)
(5)
Gaspermeablemembrane
Ir/IrOxelectrode
Ag/AgClelectrode
DuallumenPVCtube
Hydrogel
epoxy
silverepoxy
Polyurethane membrane
Anodic
+i
+ 0.6 V
-i
Cathodic
Urease
CO(NH 2 )2 CO2 + 2NH 3
H 2 O
V
IrOx
IrOx
urease
Immunosensors
Immunosensors
Immunosensors
Enzyme labeled antigen
Immobilized antibody
Antigen
Oxygen sensor
Oxygen permeable membrane
Oxygen sensor
Immunosensors
Wash the unbound antigen away and add H 2O2
Oygen is formed
Oxygen permeable membrane
Oxygen sensor
The oygen signal is lower the higher the amount of native antigen
Homework
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