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SEMINAR
O
n
ROLE OF POLYUNSATURATED
FATTY ACIDS oN BOVINE
FOlLICULOGENESIS
INTRODUCTION
Dietary fats influence the reproductive functions
Increasing energy balance
Direct action on reproductive processes
Thatcher et al., 2003
C 18:1
C 18:2
C 18:3
Safflower
12
77
<1
Cottonseed
25
21
50
Linseed (flax)
19
14
58
Corn
25
60
Tallow
15
41
Fishmeal
25
45
Olive
76
Soyabean
24
53
Sunflower
20
69
1
(Staples and Thatcher, 2005)
Plasma Progesterone
concentration
(Robinson
Cows that were fed sh oil increased
theet al., 2002)
number of medium follicles (510 mm in
diameter)
Control
E-FLAX
E-SUN
Cows, n
Oocytes, n/cow
5.20
4.88
3.75
Grade 1 oocytes, n
3.74
3.45
2.50
Grade 1 + 2 oocytes, n
4.12
3.98
3.02
3.05
3.29
2.16
49.73
47.07
No. of class 2
follicles
Size of the CL
MEG; 2.8%DM, EE
6.5%
(FW)LIN; 6.1%, EE
5.2%
FIS ; 3.4%, EE 5.4%
OIL. (L); 500gm, EE
4.9%
(Petit et al., 2002)
Control
ALA (n-3)
LA (n-6)
0.59
0.36
1.66
Number of cow
1.8
3.4
3.5
13.3
15.6
16.9
Treatment
Variables
Control
FLX
FO
P<
Total number of
oocytes aspirated
248
361
306
0.05
Oocytes
recovered/cow per
session
3.3
4.8
3.9
0.03
35
52
48
0.01
No. of cleaved
oocytes/cow per
session
1.06
1.97
1.66
0.04
(Moallem et al.,
2013)
SFA
FLX
FO
P value
C18:2 n-6
45.38
45.48
40.99
NS
C18:3 n-3
2.20
5.69
1.90
0.001
C20:5n-3
0.29
0.32
0.76
0.03
C22:6n-3
0.03
0.03
0.28
0.001
n-3
2.78
6.10
3.53
0.001
n-6
48.12
47.25
42.41
NS
2.64
4.71
2.99
0.001
n-6
41.22
33.80
31.13
0.03
Control
7.39
4.53
<0.0001
0.61
0.38
0.001
7.61
5.11
<0.001
C20:5 Eicosapentaenoic
acid
0.73
4.22
<0.0001
C22:6 Docosahexaenoic
acid
2.85
3.78
<0.01
Omega 6
15.01
9.64
<0.0001
Omega 3
4.20
8.39
<0.0001
PGE2 considered a
luteotrophic mediator
and thus facilitates
the
Establishment of
pregnancy
Not affected
(McLaren et al.,
2006)
PUFAs
PGE1
PGE2
PGE3
CONT
36.0
30.7
33.0
31.8
DGLA, 20:3,
n-6
76
8.7
15.3
125.5
20.7
64.3
14.3
85.1
EPA, 20:5, n3
29.7
28.7
41.0
16.1
Kirkup et al., 2011
(Thangavaleu . 2007)
Morula
Blastocyst
Expanded
Blastocyst
Dietary groups
SAT
FLX
SUN
P
value
64.4
77.5
89.3
76.3
88.6
115.4
65.6
93.7
132.3
0.09
0.07
0.02
93.4
97.1
0.01
(Thangavaleu .2007)
Low-fat
High-fat P
value
570
481
In vitro-fertilized oocytes
495
418
53.3
57.8
0.47
7.1
6.1
0.021
9.3
7.9
0.051
RIF (Megalac) ;
Low fat; 200g/day
High fat ; 800g/day
40-60 days after
calving
0.557
Grade 2
3.3
(35.6%)
2.36 (40.3%)
0.082
Grade 3
2.3
(32.2%)
2.1
Total cells
132.5
150.5
0.043
ICM
33.8
36.4
0.46
Cleavage rate
72.06
66.6
0.076
Blastocyst/IVF
19.4
27.4
0.004
38
0.017
(28.2%) 0.147
Development (%)
Blastocyst/cleav 29.1
ed
ALA supplementation
after 24 h
Effect PUFA on PG
ConcentrationProduction
in spent
media
after maturation for 24 h
in the presence of LA
(mM)
Intracellular cAMP
concentration in COCs
LA supplementation(100 M)
50 M ALA, 3 M forskolin,
or both
Luteinizing hormone
stimulation
high cAMP
Dekel, 1988
selective activation of PKA type II by cAMP in
cumulus cells
selective inhibition of phosphodiesterase enzyme type 4
is needed for resumption of meiosis
(Luciano.
2004)
The concentration of cAMP in cumulus cells at 3 h is an important
regulator of
cumulus expansion and oocyte maturation
ALA (50M)
AKT
PHOSPHORYLATION
Treatment of COCs
(Waleed et al.,
2009)
Embryo survival
n-3 PUFA
P-value
Estimated ovulations
17.50
15.06
NS
No. of non-fertilised
1.063
1.69
NS
1.25
<0.05
No. of morula
0.94
1.81
NS
4.18
3.81
NS
No. of transferable
embryos (grades 1 and 2)
6.60
5.88
NS
4.8
11.4
5.1
17.2
9.8
27.3
Sunflower
seed,of
rolled;
8.7
%
Proportion
cows
calving
Even though
cows received
90.2
72.7the
DM Flaxseed, rolled ; 9.0%
experimental diets for only 60 d (i.e.,
DM
from 28 d before TAI to 32 d after
EE, %DM; 7.15%
TAI), the ALA-enriched diet during
(SUNF),7.23%(FLAX)
Ambrose et al., 2006
LA supplemented
(n=8)
132d
138d
132d
Dexa- induced
labour
Control
40 hours
LA supplemented
32 hours
CONT; Megalac
-18g/day/sheep
TRET; SoyPreme190g/day/sheep
C
Gestation Length
(days)
145.2
5.2
3 week
6 week 9
week.
P
value
147.1
147.5
148.0
0.08
5.4
5.2
5.3
NS
21.6
22.6
0.05
Time to stand
31.0 27.9
(min)
C; Vegetable oil ; 32 g/ewe per day
,
S; 64 g algae /ewe per day, to provide 12 g
4. Competition of PUFAs
for PGHS
Increased amounts of EPA could result in increased
synthesis of prostaglandins of the 3 series, and
reduced synthesis of PGF2
5. Effects on gene
expression
PUFAs involve activation of nuclear transcription
factors such as peroxisome proliferator activated
receptors (PPARs)
CONCLUSION
Diets enriched in PUFA accelerates early embryonic
development in dairy cows, compared to a diet
enriched in saturated fatty acids.
Dietary fats influence the reproductive functions by
improving energy balance or direct action on
reproductive processes
PUFA has benecial effect on reproductive hormones,
oocytes
number,
quality,
maturation
and
development, decrease in early embryonic mortality,
enhanced embryo survival and neonatal behaviour.
PUFA affect the expression of genes involved in
many reproductive processes.
So the strategic supplementation of PUFA may be
used to increase the fertility in dairy animals
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