Académique Documents
Professionnel Documents
Culture Documents
ArthurRowe
TheKnowledgeBaseataSimpleLevel
Introduction
These3presentationscoverthe
fundamentaltheoryofelectronmicroscopy
Inpresentation#1wecover:
thebitsofopticsweneedtoknow
productionofanelectronbeam
whyweneedvacuum&howwegetit
thefoundationsofelectronoptics
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GinaSosinskysEMOutreachprogram:
http://emoutreach.sdsc.edu/
BasedattheSupercomputingCenteratSanDiego,thissite
includesaveryfullsetofWebbasedlecturenotes.Goodtext,but
sadly,Ginahashadproblemsgettingmanymicroscopiststoagree
tocopyrightreleasefortheirwork,soforthemomentthe
illustrationsareratherminimal.Oneslightmisstatement,itissaid
thatshadowingusuallygivesmuchworseresolutionthannegative
staining.Nottrue.
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AnalyticalImagingFacilityatAlbertEinsteinCollegeof
Medicine:
http://www.aecom.yu.edu/aif/welcome.htm
Concentratesheavilyasyouwouldexpectontheanalytical
sideofmicroscopy.Alltypesareconsidered,lightandelectron
microscopybeingintegratedtogether.Asyoumightexpectfrom
theAlbertEinstein,thissiteisafantasticresource
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HelenSaibilshomepage:
http://www.cryst.bbk.ac.uk/~ubcg16z/saibil.htm
Lovelyimagesandsomemoviesofselectedproteinbased
systems,includingchaperonin(GroELGroES)andporeforming
toxin(pneumolysin)
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UNSWsImageGallery:
http://srv.emunit.unsw.edu.au/html/gallery/gallery.htm
Slantedtowardshistologyinthebiologysection,butgoodimages
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UniversityofSussexEMsite
http://www.biols.susx.ac.uk/Home/Julian_Thorpe/cover.htm
somegoodteachingmaterialatasimplelevel,aswellassome
prettyimages.
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andyoucanofcoursegoon
almostforever,ifyouenter
electronmicroscopyintoa
searchengine(SCIRUSis
goodforscience)
sonow,ontothebasics......
axis of lens
definition of resolution
-5 mbar
also
- tungsten filaments burn out in air
- columns must be kept dust free
achieved by 2-fold pumping:
rotary (mechanical) pump + diffusion pump
or + turbo pump
cooling coils
windings
windings
soft iron
pole piece
electrons are charged, and are therefore
deflected when they cross a magnetic field
thro lens
focus
image
enlarge
- projected
the object
Gunemitselectrons
Electricfieldaccelerates
Magnetic(andelectric)fieldcontrol
pathofelectrons
Electronwavelength@200KeV
2x1012m
Resolutionnormallyachievable@
200KeV2x1010m2