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amino acids
Topics to be covered
Removal of -amino groups from amino acids
(transamination and deamination)
Enzymes and coenzymes involved during
transamination
Entry of the carbon skeletons to the TCA cycle
Amino group transport
Removal of ammonia in humans (urea cycle)
Lecture objectives
To describe the oxidation of amino acids
To explain the different ways of removal of
amino groups from amino acids
To understand the reactions and enzymes
involved in the oxidation of amino acids
To discuss the way of ammonia removal
from the body
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Nitrogen balance
In normal adults, nitrogen intake matches
nitrogen excreted.
Positive nitrogen balance, an excess of
ingested over excreted nitrogen, accompanies
growth and pregnancy.
Negative nitrogen balance, where output
exceeds intake, may follow surgery, advanced
cancer, and kwashiorkor or marasmus.
While ammonia, derived mainly from the amino nitrogen of amino acids, is highly toxic,
tissues convert ammonia to the amide nitrogen
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of nontoxic glutamine.
Metabolism of proteins
The continuous degradation and synthesis of
cellular proteins occur in all forms of life.
Each day humans turn over 12% of their total
body protein, principally muscle protein.
High rates of protein degradation occur in
tissues undergoing structural rearrangement
eg, uterine tissue during pregnancy, tadpole tail
tissue during metamorphosis, or skeletal
muscle in starvation.
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Muscle
protein
Amino acids
Glucose
glycolysis
Pyruvate
NH4+
Glutamate
Alanine
GLT
-Ketoglutarate
Blood glucose
Blood
Alanine
Glucose
Gluconeogenesis
Pyruvate
-Ketoglutarate
ALT
Glutamate
NH4+
Urea cycle
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Urea disposal
The urea produced is transported via blood to the
kidney to be filtered and excreted through urine.
A portion of the urea diffuses from the blood into the
intestine, and is cleaved to CO2 and NH3 by bacterial
urease.
In patients with kidney failure, plasma urea levels are
elevated.
The intestinal action of urease on this urea becomes a
clinically important source of ammonia, contributing to
the hyperammonemia often seen in these patients.
Oral administration of neomycin reduces the number
of intestinal bacteria responsible for excess ammonia.
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Nonessential
Alanine
Aspartate
Asparagine
Cysteine
Glutamate
Glutamine
Glycine
Proline
Serine
Tyrosine