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D I V I S I U R O G I N E KO LO G I D A N R E KO N S T R U K S I O R G A N
PA N G G U L
D E PA R T E M E N O B S T E T R I D A N G I N E K O L O G I
FA K U LT A S K E D O K T E R A N U N I V E R S I T A S H A S A N U D D I N
M A K A SS A R
2016
I
N
T
R
O
D
U
C
T
I
O
N
RING PESSARY
urinary, bowel,sexual
function
QO
L
POP
Conventional
methods
BV
Clone library
methods
Culture based
methods
AIM :
To evaluate intravaginal microbial flora
of POP patients using the ring pessary
with conventional and clone library
methods
Informed consent
Measure Vaginal pH
Specimen from
posterior fornix
Wallace Ring pessary
4 weeks later
Check pessary position
& repeat intravaginal
microbial analysis
pH-Fix 3.66.1:
MACHEREYNAGEL, Duren,
Germany
Nugent Score
+ clue cells
Nugent Score
Examined presence of BV
One trained technician who was blinded to the
origin of slides
Criteria :
0-3: normal flora
4-6 : intermediate vaginal flora
7-10 : BV
Clone library
16S rRNA gene sequencing
RESULTS
D
I
S
C
U
S
S
I
O
N
LIMITATIONS
There was a wide
variety of clinical
features among
patients, even with the
same POP-Q stage.
Original intravaginal
microbial flora (before
ring pessary therapy)
were different.
Each method of
microbial detection has
advantages and
disadvantages
CONCLUSION
TERIMA
KASIH