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Introduction
Enzymes are found in small amounts mainly within cells ,clotting
factors and digestive enzymes function naturally after secretion:
- Plasma specific Thrombin
- Secreted - Lipase, Amylase
- Intracellular - transaminases, creatine kinase
Injury or death of tissues can cause the release of tissue-specific
enzymes into the bloodstream.
Elevated enzyme levels are often indicators of tissue problems, and are
used in the diagnosis of diseases.
Enzyme activities in the body fluids are altered by pathological
processes so, its measurement is used for disease investigation
Liver enzyme:
Alanine aminotransferase (ALT; old name SGPT) is normally found
largely in the liver
Bone enzyme:
Alkaline phosphatase (ALP)
Isozymes
Multiple forms of same enzyme
Catalyse same reaction
Differ in molecular weight ,structure and
charge
Have different Km for same substrate
Important in diagnosis of diseases
Creatine kinase
Creatine kinase is associated with ATP regeneration in
muscle and nervous tissue.
Elevated blood levels of CK are used as indicators of MI,
muscular dystrophy, and stroke.
CK occurs as a dimer of 2 different subunits, M and B.
Serum Enzymes
in Disease
Myocardial infarction
Myocardial infarction
Necrosis of the myocardium, but not angina pectoris release
of CK, AST and LDH (HBD) into the circulation.
CK is the first to rise (activity within 6 h of MI ).
Total CK reaches a peak at 24-36 h.
In uncomplicated cases, CK returns to normal within 3 days.
Serum AST more slowly ( maximum activity within 48 h) and
returns to normal in 4-5 days.
No significant elevation in HBD seen for the 1st 24 h (reaches
maximum at about 3 days & remain for up to 8 days).
It is important to consider the timing of sample when
interpreting test results.
CK & HBD are useful as early and late indicators of MI, and
more specific than AST.
9
Myocardial infarction