Académique Documents
Professionnel Documents
Culture Documents
Winter 2011
Measure
from the Measure the
well to the distance for
middle of plasmids and
each band. fragments.
Plot the Use the
standard standard
curve with curve to find
these values. their sizes.
Standard Semi-Log Plot
100,000
10000
1000
100
Distance Traveled (cm)
Standard Semi-Log Plot
Distance Traveled (cm) MW (bp)
1.8 5000
Distances
1.9 4500 Standard
measured sizes of the
from gel 2.0 4000
ruler
photograph 2.1 3500 fragments
2.25 3000
2.5 2500
2.8 2000
3.15 1500
3.75 1000
4.65 500
100,000
Omit data that
doesnt show a
linear relationship.
10000
X Use the best fit
XX
X line.
X
X
X
X
1000 X
100
1 2 3 4 5
100,000
10000
X
XX
MW is X
X
~900 bp X
X
X
1000 X
Fragment
at 3.8 cm
100
1 2 3 4 5
Using Excel to
Estimate Fragment Size
Before plotting the
standard curve, use
the Excel function
=LOG10(x)
to convert MW
numbers to log
values