Académique Documents
Professionnel Documents
Culture Documents
REKAYASA GENETIKA
DAN
MOLEKUL KEHIDUPAN
1
Pokok Bahasan
The Chemistry of Heredity
The Double Helix of DNA
Cracking the Chemical Code
Protein Structure and Activity: Form and Function
The Human Genome Project
Genetically Engineered Medical Treatments
Genetically Engineered Agriculture
Cloning Mammals and Humans
The New Prometheus?
2
The Chemistry of Heredity
(Kimia Keturunan)
Konsep Dasar Genetik
Human Genome:
- 10 million million
(10x1012) cells with a
nucleus
- each cell has a complete
set of genetic instructions to
make
another you(biologically)
- 23 pairs of chromosomes
- 100,000 genes
3 parts to DNA:
7
DNA
Put all 3 groups together,
called a nucleotide
Adenosine phosphate
8
The percenst base composition of DNA
for various species
Common
Species Adenine Thymine Guanine Cytosine
Name
Homo sapiens Human 31.0 31.5 19.1 18.4
Drosophila melanogaster Fruit fly 27.3 27.6 22.5 22.5
Zea mays Corn 25.6 25.3 24.5 24.6
Neuspora crassa Mold 23.0 23.3 27.1 26.6
Escherichia coli Bacterium 24.6 24.3 25.5 25.6
Bacillus subtillis Bacterium 28.4 29.0 21.0 21.6
9
The Double Helix of DNA
X-Ray Diffraction of DNA
11
DNA
Turns out that:
Called
complementary
bases:
A=T
G=C
12
DNA Double Helix
DNA is double stranded and
is shaped like a spiral
staircase.
ATAGCCG
13
DNA Replication
14
Cracking the Chemical Code
DNA: Blueprint of Life?
How does DNA provide genetic information, where does the
information come from?
16
DNA: Blueprint of Life?
How does DNA provide genetic information, where does the
information come from?
17
DNA to Proteins
OK, the instructions are in the sequence of bases.
There are 20 amino acids
18
DNA to Proteins
OK, the instructions are in the sequence of bases.
There are 20 amino acids
19
DNA to Proteins
OK, the instructions are in the sequence of bases.
There are 20 amino acids
20
DNA to Proteins
OK, the instructions are in the sequence of bases.
There are 20 amino acids
21
Codon table
22
DNA to Proteins
Transcription: DNA to mRNA
23
Protein Structure and Activity:
Form and Function
Polypeptide
Backbone
25
Protein structure
26
Secondary structures
27
Tertiary structure
29
The Human Genome Project
What is the Human Genome Project?
32
Benefits of Human Genome Project research
improvements in medicine.
microbial genome research for
fuel and environmental cleanup.
DNA forensics.
improved agriculture and
livestock.
better understanding of
evolution and human migration.
more accurate risk assessment.
33
Genetically Engineered
Medical Treatment
Recombinant DNA: manipulating gene pools
Cut out DNA that encodes for
human insulin
39
How to create transgenic plants
Agrobacterium tumefaciens
Ti Recombinant
plasmid Insertion of gene Ti plasmid Introduction Regeneration
into plasmid using into plant of plant
restriction enzyme cells in
T DNA and DNA ligase culture
T DNA carrying new
gene within plant chromosome Plant with new trait
Restriction site
Nitrogen fixing corn,
corn that capable in Transgenic soybeans
fixing N2 which is resistance to
Contain nitrogen fixing herbicides
bacterial genes
41
Mixing Genes: Transgenic Organisms
Cloning Mammals and
Humans
Do you know these movies?
Jurassic Park
The Lost World
The Boys from Brazil
Sleeper.
Multiplicity
Invasion of the Body Snatchers
Stepford Wives
A Stolen Life
Clones
The Clones of Bruce Lee
Blade Runner
Star Wars.
Judge Dredd
American Ninja 2
Twins
Gattaca
Alien Resurrection
44
Nuclear transfer/ Somatic cell nuclear transfer
45
Dolly, 1996-2003
46
Snuppy, 2005
47
Human cloning
48
The New Prometheus?
We have found key natural compounds of life
DNA
We have clone gene and took many advantage
for human being
We have clone animals
Next logical step would be creating new organism
or cloning human the super one
However we could not removed defective gene
from the pools
And cloning or creating superman could end up
with creating a new promatheus
50
Thank you
51