Académique Documents
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Biotechnology
Objective BA011.01: Summarize
the physiological needs of animals
for growth and reproduction.
Physiological Needs of Animals
Food/ Nutrients
Animals CANNOT produce their own food
Must consume other living organisms for energy
Respiration
Converting sugars to chemical energy
Occurs in the mitochondria
Oxygen
Usually absorbed by animals from the air
Occasionally absorbed through water or
other means (ex- fish)
All animals are AEROBIC
Aka- they will die without oxygen
Physiological Needs of Animals
Water
Other than air, the single most important
factor in the survival of all animals
Animals can only last a few days at most
without water, though they can last days
without food.
Physiological Needs of Animals
Lymph System
In advanced animals
Utilizes white blood cells and antibodies to
attack any antigens in the blood of an
organism
Vaccines help the body form antibodies
more quickly, while under less stress
Animal Science and
Biotechnology
Objective BA011.02: Analyze the
impact of biotechnology on animal
disease prevention, diagnosis, and
management
Animal Disease Diagnosis
ELISA tests
Utilize antigens to determine the presence
of antibodies for a given pathogen in a
blood sample
Antibodies indicate the presence of a
particular pathogen being fought
The tests are usually produced from
antigens extracted from research animals
Animal Disease Diagnosis
Bacterial Infections
Are tested in animals by biotechnology by
culturing samples in an incubator
Different agar medias can be used to
determine the presence of different bacteria
upon examination after growth
Animal Disease Prevention and
Treatment
Biotechnology has enabled researchers
to produce animals with genetic
resistance to many pathogens
Use gene segments from naturally resistant
organisms
Find the gene in sharks that makes them
resistant to cancer for use in humans
Produce animals with gene segments coding for
the production of proteins to attack potential
parasitic organisms
Animal Disease Prevention and
Treatment
The production of antibodies in one
organism for use in another is an
important biotechnology technique used
in vaccines
Jumpstarts the immune system of an animal
Also used in humans
Animal Disease Prevention and
Treatment
The utilization of genes coding for the
production of certain medical
compounds (including antibiotics) in a
variety of livestock enables
agriculturalists to
Provide preventative medication in semi-
controlled doses to populations on a large
scale
Animal Science and
Biotechnology
Objective BA011.03: Discuss the role
of genetic engineering and
biotechnology on improving animal
breeding
Biotechnology Techniques in Animal
Breeding
Artificial Insemination
Process of extracting and diluting semen
from a male animal for use in a female
animal
Allows for outstanding genetic
characteristics to be spread through a
population rapidly with minimal expense and
high success
One ejaculate can produce more than 60 semen
straws in cattle and horses
Biotechnology Techniques in Animal
Breeding
Knockout Animals
Used to determine the function of specific
genes, by creating animals without these
genes
Biotechnology Techniques in Animal
Breeding
Cloning
Rarely used in animals
Expensive and large amounts of tissue damage
Used for research or to preserve the most
destroyed
Biotechnology Techniques in Animal
Breeding
In Vitro Fertilization
A more common method is transferring
fertilized eggs from a super-ovulated female
to other females
One female can produce many times more
offspring
Problems with Biotechnology in
Animal Reproduction
Genetic Diversity
Could possibly decrease with increased use
of biotechnology
Less diversity in breeds/ species
Most important negative aspect of increased use
of biotechnology
Problems with Biotechnology in
Animal Reproduction
Expense/ Technical Knowledge
Some processes can easily be completed
on the farm
Many techniques still require expensive
laboratory equipment or facilities.
Animal Science and
Biotechnology
Objective BA011.04: Evaluate the
function of hormones in animal
growth and body regulation
Function of Animal Hormones
Testosterone
Common in varying levels in males
Can lead to aggression- castration
Animal Science and
Biotechnology
Objective BA012.01: Practice
biotechnology techniques utilized in
animal breeding.
Performing Artificial Insemination
cells in a sample
Performing Artificial Insemination
Thawing Semen
Straws should be stored in a container
using liquid nitrogen to maintain subzero
temperatures
Straws should be quickly removed from the
container, shaken, an immediatedly placed
in a water bath at 99 F for 15 seconds
Shaking removes water from the exterior to
avoid breaking the seal
Performing Artificial Insemination
Inseminating the Female
Most methods utilize a specialized gun to
deliver the semen from the straw to the female
The inseminator (thats the person) inserts the
gun into the vagina of the female, through the
cervix, to release the semen into the uterus
The other hand of the inseminator is gloved
and inserted into the rectum to palpate the
location of the cervix and guide the gun through
without damage
Performing In Vitro Fertilization &
Embryo Transfer
Gathering Eggs
In Vitro- Eggs are usually removed from the
ovaries in large quantities through simple
surgery
Embryo Transfer- Eggs are fertilized in the
uterus of the female
Female given hormone to induce super-ovulation
During ovulation, the female is inseminated
Tools
Micromanipulator is the most important tool
Used to divide cells, remove DNA, enucleate
cells, and reinsert DNA
Animal Cloning Issues