ACETONE BUTANOL PRODUCTION

Reporters:
Baldago, Noezhel Buscado, Reyjay Niko Segovia, Maria Teresa
Bohol, Al Christian Celles, Cristel Angeline
Keywords
ABE Fermentation
Clostridium acetobutylicum
Endospore
Germination
Pyruvate Decarboxylase
Butanol Dehydrogenase
Ethanol Dehydrogenase
AcetoacetlyCOA Transferase and Acetoacetate Decarboxylase
DDGS
ACETONE
a colorless volatile liquid ketone made by
oxidizing isopropanol, used as an organic
solvent and synthetic reagent.
Organic substance with a chemical formula
C3H6O
Systematically known as PROPANONE

USES
BUTANOL
four-carbon alcohol with a formula
of CHOH, which occurs in four
isomeric structures, from a straight-
chain primary alcohol to a branched-
chain tertiary alcohol

USES
 HISTORY

In 1905, Austrian
biochemist Franz
Schardinger found
Louis Pasteur 1st that acetone could In 1916, Chaim Weizmann's
recorded butanol similarly be produced. isolation of Clostridium
production via anaerobic acetobutylicum which yieled
bacterial fermentation in more acetone and butanol
1861
DID YOU KNOW
1910 shortage of national rubber

BUTANOL ISOPRENE RUBBER

PRODUCTION OF ACETONE
Chemical Process
a. Cumene-Phenol Process/ Hock Process
developing phenol and acetone from benzene and propylene.
b. Acetate Distillation- Calcium acetate when heated at a temperature of
about 380 C. decomposes, giving acetone and a residue of calcium
carbonate.
c. Crude Oil Distillation
Biochemical process
ABE Fermentation
PRODUCTION OF BUTANOL

Butanol can also be produced

by fermentation of biomass by bacteria.
Fossil Fuel
ABE Fermentation
Acetone-Butanol-Ethanol (ABE)FERMENTATION
- a process that uses bacterial fermentation to produce acetone, n-Butanol, and
ethanol from carbohydrates such as starch and glucose.
- Class Clostridia (Family Clostridiaceae) Clostridium acetobutylicum ("Weizmann
Organism)

- produces solvents in a ratio of 3 parts acetone, 6 parts butanol to 1 part ethanol

MAJOR SUBSTRATE
METABOLIC PATHWAY
PRODUCTION

Feed Preparation
Seed Fermentation

-pH less than 4.5

-temperature 65-100C
-retention time 6days
Fermenter

-pH 4-5
-temperature 34-39C
-retention time 40-60 hrs
-nutrients NH3, H2SO4,
Lime

Chemical Rxn in fermenter

C6H10O5 + 9H2O = 3C3H6O + 6C4H10O +

C2H6O + 24CO2 + 16H2 + Biomass

Convertion of starch is 99%.

Effect of pH on the production of
Acetone and Butanol
Product Recovery

Acetone 56C
Ethanol- 78.37C
Butanol- 117.7C
Solid separation
Evaporation
DDGS Drying
Recap
Key Words

ABE Fermentation
Clostridium acetobutylicum
Endospore
Germination
Pyruvate Decarboxylase
Butanol Dehydrogenase
Ethanol Dehydrogenase
AcetoacetlyCOA Transferase and Acetoacetate Decarboxylase
DDGS