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5.1 : MENDELS
EXPERIMENT
Phenotype: observable
characteristic of the some
organism like colour, size, form and
structure
Eg: tall, short
Genotype: genetic composition of
an organism and cannot be seen
Eg: TT, tt, Tt
MONOZYGOTE AND
HETEROZYGOTE
In Mendels experiments,both pea plant in parental
generation were pure-breeding.
Therefore, the tall plant had two alleles for tallness(TT)
The short plant had two alleles for shortness(tt)
This called homozygote.
The tall plant produced gametes which carried the allele T
and and short plant which carried the allele t will produced
all tall plant.
They had one allele for tallness and one allele for shortness(Tt)
This called heterozygote.
Three quarters of the offspring in the F2 generation were tall
and one quarter was short.
Hence, the phenotypic ratio is 3:1
The genotype of tall pea plant in F2 generation is TT and Tt
while short pea plant is tt.
Hence, the genotypic ratio is 1 TT : 2 Tt : 1 tt
MENDELS LAW OF 1 ST
INHERITANCE
Monohybrid Cross:
-cross carried out by Mendel involves only one character
Involves only one pair of alleles
Mendel formulated his 1st Law of inheritance
This called the Law of Segregation. It states:
-The members of each pair of alleles separate or segregate
during the formation of gametes only one allele can be carried
in a single gamate.
Monohybrid inheritance:
-inheritance involving a single characteristic determined by one
genes
Dihybrid inheritance:
-a cross which involves two pairs of alleles determining two
characteristics
MENDELS 2
LAW OF ND
INHERITANCE
It is called Law of Indipendent
Assortment
It states:
- two or more pairs of alleles segregate
independently of one another during the
formation of gametes.
MONOHYBRID CROSS
In the initial set of experiments, Mendel
concentrated only on the pattern of
inheritance of a single pair of
contrasting characters. This pattern of
inheritance involving only one pair of
contrasting characters is known as
monohybrid inheritance.
In the first set of experiments, Mendel
conducted cross-pollination between a
pure-breeding tall plant and a pure-
breeding dwarf plant. He collected the
seeds from this cross pollination and
allowed them to germinate. All the
resulting plants were found to be tall.
Based on these results, Mendel came to
the conclusion that in a cross-involving
two contrasting characters, only one
character expresses itself in the next
generation. Mendel called the character,
which expressed as dominant character
and the character, which failed to
express, as recessive character. This
idea came to be known as the principle
of dominance (first law).
DIHYBRID
CROSS
DIHYBRID CROSS
Figure 10-7
For each dihybrid cross, Mendel cross-fertilized true-
breeding plants that were different in two characters. Then
he allowed the F1 hybrids to self-fertilize. In this case, the
two characters, seed color and shape, are displayed by the
first stage of each new generation, the seed (pea).
ANOTHER
EXAMPLE
F1 GENERATION
GW Gw gW gw
GGww
GGWw GgWw Ggww
Gw (Yellow,
Male (Yellow, (Yellow, (Yellow,
wrinkled
round) round) wrinkled)
)
Ggww
GgWw (Yellow, ggWw ggww
gw
(Yellow, wrinkled (Green, (Green,
round) ) round) wrinkled)
PUNNET SQUARE
The genotypes and phenotypes
resulting from various
combination of gametes can be
easily determined by Punnet
squares, devised by Reginald C.
Punnet (1875 1967). Hence each
of the possible gametes is placed
in an individual column or a row,
with vertical column representing
the female and horizontal row the
male parent. The gametes are
then arranged in all possible
combinations and the resulting
genotypes are entered in the
boxes along with the phenotypes.
DIHYBRID CROSS USING SCHEMATIC
DIAGRAM AND PUNNET SQUARE