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Plasma Membrane
Double membrane
surrounding the
chromosomes and the
nucleolus
Pores allow specific
communication with the
cytoplasm
The nucleolus is a site
for synthesis of RNA
making up the Nucleus
ribosome
Surrounded by a double
membrane with a series
of folds called cristae
Functions in energy
production through
metabolism
Contains its own DNA,
and is believed to have
originated as a captured
bacterium Mitochondria
Surrounded by a double
membrane, containing
stacked thylacoid
membranes.
Responsible for
photosynthesis, the
trapping of light energy
for the synthesis of sugars
Contains DNA, and like
mitochondria is believed
to have originated as a Chloroplasts (plastids)
captured bacterium.
A network of interconnected
membranes forming
channels within the cell
Covered with ribosomes
(causing the "rough"
appearance) which are in the
process of synthesizing
proteins for secretion or
localization in membranes
Ribosomes
Vacuoles
Produce and degrade
hydrogen peroxide, a
toxic compound that
can be produced during
metabolism
Peroxisomes or Microbodies
Cytoplasm fluid mosaic
Cell wall
Capsule
Glycocalyx
Bacteria
Characteristics of Prokaryotic
Cells
Molecules surrounded by a membrane and cell
wall
Lack characteristic eukaryotic subcellular
membrane enclosed "organelles
May contain membrane systems
May have photosynthetic pigments like in
cyanobacteria ("blue-green bacteria")
Some have external whip-like flagella for
locomotion or hair like pili for adhesion
Come in multiple shapes: cocci (round),
baccilli (rods), and spirilla or spirochetes
(helical cells).
External layers
Capsule
Glycocalyx
Appendages
Flagella
Pili/Fimbriae
The Cell Wall
Cell envelope
Cell membrane and peptidoglycan layer
The cell walls of gram-positive bacteria are
made up of twenty times as much murein or
peptidoglycan than gram-negative bacteria.
These complex polymers of sugars and amino
acids cross-link and layer the cell wall.
The thick outer matrix of peptidoglycan,
teichoic acid, polysaccharides, and other
proteins serve a number of purposes,
including membrane transport regulation, cell
expansion, and shape formation.
Because gram-positive bacteria have more
layers of peptidoglycan in their cell walls than
gram-negative, they can retain the dye.
Gram Positive bacteria
(No cell wall)
Examples of mycoplasmic bacteria:
urethritis
Inside the Cell Wall
cytoplasm
Nuclear Area
Not surrounded by membrane
Nucleoid or nuclear body
Ribosomes
- Combination of a special type of RNA called
ribosomal RNA or rRNA(60%) and protein(40%).
- Characterized by S, Svedberg units
Rates molecular sizes through centrifugation.
Bacterial rating:
70S
w/ 2 smaller subunits
30S
50S
Inclusion bodies
For food
For water
Endospore/Spore
Diplo
In Chain
Strepto
In groups/bunch
Staphylo
Others
Tetrad
sarcinae
Bacterial Growth
Increase in number
Duplicates by binary fission
Terminologies
Colony increased bacterial growth that has
come visible to the naked eye, like pus.
Culture Bacterial growth made on a petri
dish(glassware with bottom and cover plates).
Pure Culture contains only 1 type of bacteria
Mixed Culture contains more than 1 type of
bacteria
Media/Medium prepared in the laboratory
as solid (w/ agar) or liquid ( broth) where
bacteria are grown.
Phases of Bacterial Growth
Lag
Log
Stationary
Death
Phase 1
Lag Phase
Introduction of bacterium
Inoculation of bacterium
Phase 2
Logarithmic
Surge of growth
Number of bacteria increase at a fast pace ( 1
becomes 2, 2 becomes 4, 4 becomes 8and so on)
Phase 3
Stationary Phase
Number of reproduced cells = number of dying
cells
Phase 4
Death
Number of dying cells is more than the number of
reproduced cells
140000
120000
100000
80000
60000
40000
20000
0
Day 1 - 2 Day 3 - 4 Day 5 - 6 Day 7-8 Day 9-10 Day 11
Bacterial Growth
Requirements
Physical
Optimal pH
Optimal temperature
Osmotic Pressure
Chemical
Oxygen
Carbon
Nutrients and Minerals
Optimal pH
Optimal means favorable
pH refers to the basicity or acidity of a
substance
pH must therefore be favorable to the
bacteria
Usual pH requirement: 7
Acidophiles survive acidic environ
Optimal Temperature
Usual temp for pathogens: normal body
temp37 deg C
Psychrophile cold
Mesophile body temp range (normal)
Thermophile hot
Extreme thermophile very hot
Osmotic Pressure
Iso osmotic: same pressure as body fluids
0.9% NaCl