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GDN202
PRACTICAL ANALYSIS USING SPECTROPHOTOMETER
Blood tests
Blood glucose
Liver Functions
Kidney Functions
Colorimetric
estimation of
blood glucose
ILO OF THE CURRENT TOPIC:
WBCs
Platelets
SPECIALIZED CELLS:
Platlets
PLASMA COMPOSITION
Water 90%
Plasma proteins
Other solutes
BLOOD SAMPLE PROCESSING
Difference between
plasma and serum?
serum = plasma clotting factors
PLASMA AND SERUM
Differences Plasma Serum
Blood is allowed to
Blood is mixed with
clot at room
anticoagulant
Preparations temperature for 15`-30`
centrifugation The
centrifugation The
supernatant fluid is then
supernatant fluid is then
separated.
separated.
Fibrinogen &
Present Absent
Prothrombin
More turbid Is clearer
Appearance
(more protein) (fewer proteins)
Plasma less preferred
Preference in clinical Serum is the most
(the interferences by
chemistry preferred.
anticoagulants.
BLOOD COMPOSITION
USES OF BLOOD TESTS
Assessing organs
S B
T
standard
Serum/pla ___ ___ 0.5ml
sma
glucose
1 ml 1 ml 1 ml
reagent
Step 3: for all ; Pipette 1ml of the
glucose reagent into the previous 3
test tubes.
Step 4: Pipette 0.5ml of the given Test
sample-Standard-DW into their corresponding
dry test tubes
o o o
Absorbance of Test
----------------------- X Concentration of standard
Absorbance of standard
Remember
the check List
Students will
be divided
into groups
Students will
examine urine
sample
Students will
estimate blood
glucose levels in the
provided samples
Write final
comment
MCQ
A. 50 mg/dl to 69 mg/dl
B. 70 mg/dl to 99 mg/dl
C. 100 mg/dl to 125 mg/dl
D. 126 mg/dl or higher on two separate
tests
WHICH OF THE FOLLOWING CONFIRMED VALUES
MEET THE DIAGNOSTIC THRESHOLD FOR
DIABETES?
A. fasting blood glucose equal to or greater
than 140mg/dl
B. random glucose greater than 160 mg/dl
C. 2 hour post prandial glucose greater than
or equal to 126 mg/dl
D. fasting blood glucose equal to or greater
than 126mg/dl
Which endocrine imbalance would cause
hypoglycemia?
A) hypersecretion of insulin
B) hypersecretion of glucagon
C) hypersecretion of cortisol
D) hypersecretion of growth hormone
CASE 1