Enzyme Technology

(Session No 3)
Written by: Dr Chan Mieow Kee
Highest Qualification: PhD
Designation: Senior Lecturer
 Last updated: 27/02/2018

Contact Hours

Total Student
Lecture Tutorial Self – Study Library Search Assignment Exam Learning Time
(hours)

35 14 51 20 5 YES 120

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Learning Outcomes
• Discuss the concept of biocatalysts, the immobilization
techniques and the industrial applications of enzymes

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Definitions
 Enzyme:

 Cofactor:

 Coenzyme:

 Holoenzyme:

 Apoenzyme:

 Isozyme:

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Industrial Applications of Enzymes

• Describe the use of enzymes in the following
industries:-
a) Pharmaceutical
b) Biotech R & D
c) Diagnostic
d) Biocatalyst
e) Food & beverage
f) Biofuel
g) Animal feed

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Enzymes as Biocatalysts
 Collision theory:

 Transition state theory:

 Rate of reaction can be increased by:
 Increasing the temperature
 Reducing the activation energy

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Enzyme Specificity
•Fischer lock and key hypothesis
•Group specificity
•Stereochemical specificity

•Substrate specificity

•Product specificity •Koshland induced fit hypothesis

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Enzyme Kinetics
 Enzyme kinetics describes the rate of reaction.
 Three possible enzymatic reactions:
 Soluble enzyme - Insoluble substrate
 Insoluble enzyme - Soluble substrate
 Soluble enzyme - Soluble substrate (homogeneous reaction)

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Enzyme Kinetics
 Michaelis Menten Plot

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Example 1
Enzyme invertase is used to inverse sugar in a laboratory
reactor and the data is shown in the following table: -
Find:
 The maximum rate of product formation, vmax.
 Michaelis-Menten constant, Km.

Sugar concentration 70 63 58 52 44 36 25 17 12
moles/m3
Reaction rate 14.375 14.220 14.076 13.880 13.550 13.100 12.130 10.890 9.610
moles/m3h

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Compulsory page

References:
Shuler, M. L. and Kargi, F. 2002, Bioprocess Engineering –
Basic Concepts, 2nd edn, Prentice Hall.

Katoh, S. and Yashida, F. 2009, Biochemical Engineering:

A Textbook for Engineers, Chemists and Biologists, Wiley-
VCH Verlag GmbH & Co. KGaA, Weinheim.

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