Microbial Cultivation

(Session No 7)
Written by: Dr Chan Mieow Kee
Highest Qualification: PhD
Designation: Senior Lecturer
 Last updated: 11/03/2018

Contact Hours

Total Student
Lecture Tutorial Self – Study Library Search Assignment Exam Learning Time
(hours)

35 14 51 20 5 YES 120

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Learning Outcomes
• Apply the knowledge of microbiology in analyzing simple
bioreactor systems, including chemostats and enzyme
batch reactors

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Cell growth in continuous culture

Chemostat

Turbidostat

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Problem 1
A new strain of yeast is cultured in a chemostat. Assume

Calculate:
a) YM X/S
b) ms
c) kd
d) µm
e) Ks
By using the data in the following table:-

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Problem 1
Conditions:
Influent substrate concentration = 800mg/L
Excess oxygen at pH 5.5 and T= 35oC

Dilution rate (1/h), Carbon substrate Cell concentration

D conc. (mg/L), S (mg/L), X
0.1 16.7 366
0.2 33.5 407
0.3 59.4 408
0.4 101.0 404
0.5 169.0 371
0.6 298.0 299
0.7 702.0 59

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Problem 2
The following equation shows the specific growth rate for the
inhibited growth in a chemostat.

So=10 g/L
Ks=1 h/L
I= 0.05 g/L
YMX/S= 0.1 g cells/g subs
Xo=0
KI=0.01 g/L
µm=0.5h-1
kd= 0

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Problem 2
a) When I = 0, find X and S as a function of D.
b) In the presence of inhibitor, find the effluent substrate
concentration and X as a function of D.
c) Find the cell productivity, DX as a function of dilution
rate.

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Feeding Regimes in Fed Batch Processes

Feeding strategy Advantages Disadvantages

Variable

Continuous

Intermittent

Incremental

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Compulsory page

References:
Shuler, M. L. and Kargi, F. 2002, Bioprocess Engineering – Basic
Concepts, 2nd edn, Prentice Hall.

Katoh, S. and Yashida, F. 2009, Biochemical Engineering: A Textbook

for Engineers, Chemists and Biologists, Wiley-VCH Verlag GmbH &
Co. KGaA, Weinheim.

Rao, D. G. 2010, Introduction to Biochemical Engineering, 2nd end,

McGraw Hill.

Nair, A. J. 2010. Introduction to Biotechnology and Genetic

Engineering, Infinity Science Press LLC.

Patrick, S. M. and Finn, B. 2008. Modes of Fermenter Operation. In:

Practical Fermentation Technology, Mcneil, B. and Harvey, L. M. (Eds.)
John Wiley & Sons, Ltd.

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